A5101564752- Advanced biosensing and bioanalysis techniques
- DNA and Biological Computing
- Biosensors and Analytical Detection
- Cancer Genomics and Diagnostics
- Modular Robots and Swarm Intelligence
- DNA and Nucleic Acid Chemistry
- Gene expression and cancer classification
- Single-cell and spatial transcriptomics
- Probiotics and Fermented Foods
- Bacterial Genetics and Biotechnology
- Nanocluster Synthesis and Applications
- Antimicrobial Peptides and Activities
- Rabies epidemiology and control
- Genomics and Phylogenetic Studies
- Synthesis and Characterization of Heterocyclic Compounds
- CRISPR and Genetic Engineering
- RNA Interference and Gene Delivery
- SARS-CoV-2 detection and testing
- Herbal Medicine Research Studies
- Gene Regulatory Network Analysis
Shenzhen Technology University
2024
Shenzhen University Health Science Center
2022-2024
Tianjin University
2017-2020
Huazhong Agricultural University
2020
Huazhong University of Science and Technology
2018-2019
Qingdao University of Science and Technology
2018-2019
Qingdao Center of Resource Chemistry and New Materials
2019
University of Utah
2013
Abstract Sensitive and specific DNA hybridization is essential for nucleic acid chemistry. Competitive composition of probe blocker has been the most adopted design its relatively high sensitivity specificity. However, specificity were inversely correlated over length concentration strand, making optimization process cumbersome. Herein, we construct a theoretical model competitive hybridization, which disclose that both thermodynamics kinetics contribute to inverse correlation. Guided by...
In DNA data storage, the massive sequence complexity creates challenges in repeatable and efficient information readout. Here, our study clearly demonstrated that PCR created significant amplification biases due to its inherent mechanism of inefficient priming, product-as-template, error-spreading prone, which greatly hinder subsequent applications such as retrieval DNA-based storage. To mitigate bias, we recruited an isothermal by combining strand displacement (SDA) with magnetic beads (MB)...
Abstract DNA emerged as a novel potential material for mass data storage, offering the possibility to cheaply solve great storage problem. Large oligonucleotide pools demonstrated high of large-scale in test tube, meanwhile, living cell with fidelity information replication. Here we show mixed culture bacterial cells carrying large oligo pool that was assembled high-copy-number plasmid presented stable storage. The underlying principle explored by deep bioinformatic analysis. Although...
A single G-quadruplex forming sequence from the human telomere can adopt six distinct topologies that are inter-convertible under physiological conditions. This presents challenges to design ligands show selectivity and specificity towards a particular conformation. Additional complexity is introduced in differentiating multimeric G-quadruplexes over monomeric species, which would be able form single-stranded 3' ends of telomeres. few have been reported bind dimeric quadruplexes, but their...
Vaccination is the most feasible way of preventing rabies, an ancient zoonosis that remains a major public health concern globally. However, administration inactivated rabies vaccination without adjuvants always inefficient and necessitates four to five injections. In current study, we explored adjuvant characteristics cordycepin, bioactive component Cordyceps militaris, boost immune responses against commercially available vaccine. We found cordycepin could stimulate stronger phenotypic...
8-Oxo-7,8-dihydro-2′-deoxyguanosine (dOG), a well-studied oxidation product of 2′-deoxyguanosine (dG), is prone to facile further forming spiroiminodihydantoin 2′-deoxyribonucleoside (dSp) in the nucleotide pool and single-stranded oligodeoxynucleotides (ODNs). Many methods for quantification damaged lesions genome rely on digestion DNA with exonucleases or endonucleases dephosphorylation followed by LC-MS analysis resulting nucleosides. In this study, enzymatic hydrolysis dSp-containing...
A catalytic DNA circuit-programmed and enzyme-powered autonomous machine was proposed for one-step, isothermal dual-level amplified detection of nucleic acids.
Abstract We present here novel computational techniques for tackling four problems related to analyses of single-cell RNA-Seq data: (1) a mixture model coping with multiple cell types in population; (2) truncated handling the unquantifiable errors caused by large numbers zeros or low-expression values; (3) bi-clustering technique detection sub-populations cells sharing common expression patterns among subsets genes; and (4) small distinct patterns. Through case studies, we demonstrated that...
Abstract In DNA data storage, the massive sequence complexity creates challenges in repeatable and efficient information readout. Here, our study clearly demonstrated that canonical polymerase chain reaction (PCR) created significant amplification biases, which greatly hinder fast stable retrieving from hundred-thousand synthetic sequences encoding over 2.85 megabyte (MB) digital data. To mitigate bias, we adapted an isothermal for low-bias of pool with complexity, named new method reading...
Abstract DNA emerged as novel material for mass data storage, the serious problem human society is facing. Taking advantage of current synthesis capacity, massive oligo pool demonstrated its high-potential in storage test tube. Herein, mixed culture bacterial cells carrying that was assembled a high copy plasmid presented stable large scale storage. Living fabricated by multiple-steps process, assembly, transformation and culture. The underlying principle explored deep bioinformatic...