Graphene has unique mechanical, electronic, and optical properties, which researchers have used to develop novel electronic materials including transparent conductors ultrafast transistors. Recently, the understanding of various chemical properties graphene facilitated its application in high-performance devices that generate store energy. is now expanding territory beyond applications toward biomedical areas such as precise biosensing through graphene-quenched fluorescence,...

Carbon-based materials, including graphene and carbon nanotubes, have been considered attractive candidates for biomedical applications such as scaffolds in tissue engineering, substrates stem cell differentiation, components of implant devices. Despite the potential these only limited information is available regarding cellular events, viability, adhesion, spreading, that occur when mammalian cells interface with carbon-based nanomaterials. Here, we report behaviors cells, specifically...

Among various nanoparticles, the silica nanoparticle (SiNP) is an attractive candidate as a gene delivery carrier due to advantages such availability in porous forms for encapsulation of drugs and genes, large surface area load biomacromolecules, biocompatibility, storage stability, easy preparation quantity with low cost. Here, we report on facile synthesis monodispersed mesoporous nanoparticles (MMSN) possessing very pores (>15 nm) application plasmid DNA human cells. The aminated MMSN...

MicroRNA (miRNA) is an important small RNA which regulates diverse gene expression at the post-transcriptional level. miRNAs are considered as biomarkers since abnormal of specific associated with many diseases including cancer and diabetes. Therefore, it to develop biosensors quantitatively detect miRNA levels. Here, we a nanosized graphene oxide (NGO) based sensor, allows quantitative monitoring target levels in living cells. The strategy on tight binding NGO peptide nucleic acid (PNA)...

Photosensitizers (PSs) are light‐sensitive molecules that highly hydrophobic, which poses a challenge to their use for targeted photodynamic therapy. Hence, considerable efforts have been made develop carriers the delivery of PSs. Herein, novel design is described biocompatible, fluorescent, folic acid (FA)‐functionalized carbon nanodots (CDs) as PS zinc phthalocyanine (ZnPc) achieve simultaneous biological imaging and FA modified on PEG‐­passivated CDs (CD‐PEG) FA‐positive cancer cells,...

Most individuals with Down syndrome show early onset of Alzheimer disease (AD), resulting from the extra copy chromosome 21. Located on this is a gene that encodes dual specificity tyrosine phosphorylation-regulated kinase 1A (DYRK1A). One pathological hallmarks in AD presence neurofibrillary tangles (NFTs), which are insoluble deposits consist abnormally hyperphosphorylated Tau. Previously it was reported Tau at Thr-212 residue phosphorylated by Dyrk1A vitro. To determine physiological...

Abstract Most individuals with Down Syndrome (DS) show an early‐onset of Alzheimer’s disease (AD), which potentially results from the presence extra copy a segment chromosome 21. Located on 21 are genes that encode β‐amyloid (Aβ) precursor protein ( APP ), key involved in pathogenesis AD, and dual‐specificity tyrosine(Y)‐phosphorylation regulated kinase 1A DYRK1A proline‐directed plays critical role neurodevelopment. Here, we describe potential mechanism for regulation AD pathology DS brains...

Matrix-assisted laser desorption/ionization mass spectrometry has been considered an important tool for various biochemical analyses and proteomics research. Although addition of conventional matrix efficiently supports analytes with minimal fragmentation, it often results in high background interference misinterpretation the spatial distribution biomolecules especially low-mass regions. Here, we show design, systematic characterization, application graphene oxide/multiwalled carbon...

Among various nanoparticles, mesoporous silica nanoparticles (MSNs) have attracted extensive attention for developing efficient drug-delivery systems, mostly due to their high porosity and biocompatibility. However, the small pore size, generally below 5 nm in diameter, potential drugs that are loaded into been limited molecules. Herein, a interfering RNA (siRNA) delivery strategy based on MSNs possessing pores with an average diameter of 23 is presented. The siRNA regarded as powerful gene...

The multifunctional DNAzyme (Dz) delivery system is developed based on nano-sized graphene oxide (nGO) for simultaneous detection and knockdown of the target gene. Dz/nGO complex allowed convenient monitoring HCV mRNA in living cells silencing gene expression by Dz-mediated catalytic cleavage concurrently.

A GO-to solution: simple graphene oxide (GO)-based assay to screen for selective inhibitors of a hepatitis C virus (HCV) helicase along with severe acute respiratory syndrome coronavirus (SARS CoV) was tested (see scheme). single found five highly the HCV orthologous SARS CoV helicase. Some these hits were validated using same GO-based assay. Worldwide, over 170 million people have infections.1 Chronic infection leads liver diseases such as cirrhosis and hepatocarcinoma is major reason...

We report the development of potential theranostic agents for cardiovascular disease that are based on high-density lipoprotein-like magnetic nanostructures (HDL-MNS). The HDL-MNS offer prospects diagnosis via noninvasive resonance imaging anatomic detection and also serve as effective cholesterol efflux to address atherosclerotic vascular lesions. synthesized by adding phospholipids HDL-defining apolipoprotein A1 surface (MNS) mimic some aspects natural HDL particles. From a diagnostic...

Magnetic nanostructures (MNS) have emerged as promising functional probes for simultaneous diagnostics and therapeutics (theranostic) applications due to their ability enhance localized contrast in magnetic resonance imaging (MRI) heat under external radio frequency (RF) field, respectively. We show that the "theranostic" potential of MNS can be significantly enhanced by tuning core composition architecture surface coating. Metal ferrite (e.g., MFe2O4) nanoparticles ∼8 nm size nitrodopamine...

Biocompatible and robust gold nanoparticles (AuNPs) were prepared via a simple synthetic strategy employing dextran as reducing agent surface coating material. Cross-linking amination of the surface-coated significantly improved colloidal stability AuNPs rendered more amenable to bioconjugation.

Plasmonic nanostructures with tunable optical properties and their designed spatial arrangements can facilitate a variety of application ranging from plasmonics to biosensors unprecedented sensitivity. Here we describe facile versatile method for fabricating plasmonic substrates based on the reshaping metal nanocrystals. Anisotropic etching redeposition Ag atoms mediated by halide ions transformed nanoprisms deposited two- or three-dimensional surfaces in solution into an oblate spheroidal...

Here we report design of a smart nanoconstruct that can be used as multimodal theranostic platform for imaging and therapy applications. Decorated with two sizes magnetic nanostructures (MNS) on thermoresponsive nanosized hydrogel (NG), the NG-MNS shows dual-mode contrast enhancement ability in MRI thermo-chemo therapeutic under an RF field. Because unique where all MNS are at exterior nanoconstruct, no compromise physical properties their effect thermal activation has been observed. In...

Pattern of events: A simple and flexible method has been developed for patterning cell adhesion ligands. Locally erasing self-assembled monolayers with tri(ethyleneglycol) groups on a gold substrate by using MALDI-TOF MS nitrogen laser filling the exposed surface an alkanethiol presenting carboxylic acid enables subsequent immobilization maleimide peptide, which can then recognize cells (see scheme).

Here we report the development of a “natural” MRI contrast agent with tunable Fe loading and magnetic core for resonance imaging.

Abstract MicroRNAs (miRNAs) are important regulatory RNAs that control gene expression in various biological processes. Therefore, over the disease-related miRNA is both for basic research and a new class of therapeutic modality to treat serious diseases such as cancer. Here, we present high-throughput screening strategy identify small molecules modulate living cells. The screen enables simultaneous monitoring phenotypic cellular changes associated with by measuring quantitative fluorescent...

B-cell lymphoma cells depend upon cholesterol to maintain pro-proliferation and pro-survival signaling via the receptor. Targeted depletion of is an attractive therapeutic strategy. We report here high-density lipoprotein mimicking magnetic nanostructures (HDL-MNSs) that can bind high-affinity HDL receptor, scavenger receptor type B1 (SR-B1), interfere with flux mechanisms in SR-B1 positive cells, causing cellular depletion. In addition, MNS core be utilized for its ability generate heat...