Among various nanoparticles, the silica nanoparticle (SiNP) is an attractive candidate as a gene delivery carrier due to advantages such availability in porous forms for encapsulation of drugs and genes, large surface area load biomacromolecules, biocompatibility, storage stability, easy preparation quantity with low cost. Here, we report on facile synthesis monodispersed mesoporous nanoparticles (MMSN) possessing very pores (>15 nm) application plasmid DNA human cells. The aminated MMSN...

MicroRNA (miRNA) is an important small RNA which regulates diverse gene expression at the post-transcriptional level. miRNAs are considered as biomarkers since abnormal of specific associated with many diseases including cancer and diabetes. Therefore, it to develop biosensors quantitatively detect miRNA levels. Here, we a nanosized graphene oxide (NGO) based sensor, allows quantitative monitoring target levels in living cells. The strategy on tight binding NGO peptide nucleic acid (PNA)...

Matrix-assisted laser desorption/ionization mass spectrometry has been considered an important tool for various biochemical analyses and proteomics research. Although addition of conventional matrix efficiently supports analytes with minimal fragmentation, it often results in high background interference misinterpretation the spatial distribution biomolecules especially low-mass regions. Here, we show design, systematic characterization, application graphene oxide/multiwalled carbon...

Among various nanoparticles, mesoporous silica nanoparticles (MSNs) have attracted extensive attention for developing efficient drug-delivery systems, mostly due to their high porosity and biocompatibility. However, the small pore size, generally below 5 nm in diameter, potential drugs that are loaded into been limited molecules. Herein, a interfering RNA (siRNA) delivery strategy based on MSNs possessing pores with an average diameter of 23 is presented. The siRNA regarded as powerful gene...

Graphene oxide (GO) is known to interact with single-stranded nucleic acids through pi-stacking interactions and hydrogen bonds between the nucleobases hexagonal cells of GO. It also quenches fluorescence when fluorophore comes near GO mesh. When (ss) regions either DNA or RNA are present, those were adsorbed onto surface a quenching located proximally surface. We demonstrated that bound can be readily dissociated from by disrupting bonding urea, which was confirmed measurement gel...

The paper reports a facile one-pot synthesis of core@shell nanoparticles (NPs) composed Au core and graphene oxide nanocolloid (GON) shell. Unique properties NPs GON can be incorporated into single nanohybrid structure to provide desirable functions for theranosis such as localized surface plasmon resonance, Raman scattering, amphiphilic surface, photothermal conversion. Synthesis Au@GON is achieved by simple reaction in aqueous phase utilizing reducing stabilizing agent without any...

Cancer immunotherapy, aimed at reinvigorating the immune system to induce a lasting anticancer response, has emerged as promising approach for counteracting evasive tactics of cancer cells. This study delves into innovative realm stimuli-responsive polymeric nanomedicines in context immunotherapy. By capitalizing on intricate landscape tumor microenvironment (TME), which orchestrates suppression and progression, offer tailored strategy enhance therapeutic interventions. The aberrant features...

Long gold rods were directly grown on a reduced graphene oxide (RGO) surface by seed-mediated and seedless approaches for the fabrication of novel Raman scattering platform. The signal from RGO was significantly enhanced 34-fold at tip rod.

The multifunctional DNAzyme (Dz) delivery system is developed based on nano-sized graphene oxide (nGO) for simultaneous detection and knockdown of the target gene. Dz/nGO complex allowed convenient monitoring HCV mRNA in living cells silencing gene expression by Dz-mediated catalytic cleavage concurrently.

New cis-strapped calix[4]pyrrole derivatives 12, 13, and 19 trans-strapped systems 14 15 bearing isophthalate-derived diamide spacers linked to the tetrapyrrolic core have been synthesized characterized by spectroscopic means. The anion-binding behavior of these receptors was investigated proton NMR spectroscopy isothermal titration calorimetry (ITC). A 2:1 binding stoichiometry observed under conditions analysis but not at lower concentration regime used for ITC. As gauged from both sets...

We developed a surface-chemistry-based approach to investigating the influence of surface functionalization on growth gold nanostructures graphene thin films by utilizing various pyrene derivatives presenting different functional groups. Among surface-modifying molecules, decylpyrene (DP) yielded highest content rods (average 22 +/- 4%) among when was pretreated with DP prior nanostructure growth. The improved yield enhanced several physical properties such as electrical conductivity and...

African swine fever virus (ASFV) causes a highly contagious and fatal disease affecting both domesticated wild pigs. Substandard therapies inadequate vaccinations cause severe economic damages from pig culling removal of infected carcasses. Therefore, there is an urgent need to develop rapid point-of-use approach that assists in avoiding the spread ASFV reducing loss. In this study, we developed colorimetric sensing platform based on dual enzymatic amplification combined clustered regularly...

Binding straps: Chiral calix[4]pyrroles 1 bearing an (R)- or (S)-binol-derived strap on one side of the tetrapyrrolic core (see picture) were synthesized and characterized. The resulting systems bind selected chiral carboxylate anions (shown in red) with high affinity acetonitrile while at same time exhibiting enantiomeric discrimination. PB=2-phenylbutyrate. Supporting information for this article is available WWW under http://www.wiley-vch.de/contents/jc_2002/2007/z604161_s.pdf from...

ViSiON (visualization materials composed of silicon-based optical nanodisks) is presented, which offers a unique combination near-infrared (NIR) properties and biodegradability. Initially, numerical simulations are conducted to calculate the total extinction scattering effects by diameter-to-thickness ratio, predicting precise control over its in NIR region. A top-down patterning technique employed synthesize with accurate diameter thickness control. 50 nm exhibits 400 times higher than that...

Photodynamic therapy (PDT) is a noninvasive strategy to treat diseases by light-triggered activation of photosensitizer (PS). One aim the recent researches on PDT overcome limitation conventional improving selective PS targeted region. Here, microRNA (miRNA)-responsive drug system developed which focuses role endogenous miRNA as an internal cancer specific stimulus for initiating release in treatment. The present consists chlorin e6 (Ce6) conjugated peptide nucleic acid (PNA) having...

Exogenous contrast agents in photoacoustic imaging help improve spatial resolution and penetration depth enable targeted molecular imaging. To screen efficient signaling materials as agents, we propose a light absorption-weighted figure of merit (FOM) that can be calculated using material data from the literature numerically simulated absorption cross-sections. The FOM shows Ti nanodisc has conversion performance similar to an Au better than Pt nanodisc. results Ti, Au, nanodiscs, which are...

Diverse strategies have been developed to visualize latent fingerprints (LFPs) that are undetectable by the naked eye. Among them, fluorescence-based approaches emerged as an attractive method for enabling high-resolution LFP imaging. However, use of fluorescent probes detection remains challenging due cumbersome processing, low selectivity, and high background interference. Here, we demonstrate highly efficient, sensitive, background-free with dual-color emission arising from manganese...

The mesoporous silica nanoparticles (MSN) having different pore sizes were synthesized and utilized for siRNA delivery system capable of controlling kinetics RNA interference.

To date, graphene oxide (GO), an oxidized version of graphene, has been utilized in many research areas including bioapplications such as drug delivery and bioanalysis. Unlike other spherical or polygonal nanomaterials, GO exhibits a sheet-like structure, which itself suggests interesting applications based on its shape. Here we show that can protect cells from internalization toxic hydrophobic molecules, nanoparticles, nucleic acids siRNA plasmid DNA by interacting with cell surface lipid...

The evaporation induced self-assembly of nanomaterials has emerged as one the important approaches to fabricate various ordered nanostructures with enhanced optical properties.

Abstract MicroRNAs (miRNAs) are important regulatory RNAs that control gene expression in various biological processes. Therefore, over the disease-related miRNA is both for basic research and a new class of therapeutic modality to treat serious diseases such as cancer. Here, we present high-throughput screening strategy identify small molecules modulate living cells. The screen enables simultaneous monitoring phenotypic cellular changes associated with by measuring quantitative fluorescent...