A5079132333- Neuroscience and Neuropharmacology Research
- Neurogenesis and neuroplasticity mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Retinal Development and Disorders
- Angiogenesis and VEGF in Cancer
- Nerve injury and regeneration
- Bone and Dental Protein Studies
- Axon Guidance and Neuronal Signaling
- Cytokine Signaling Pathways and Interactions
- Lymphatic System and Diseases
- Nitric Oxide and Endothelin Effects
- Mitochondrial Function and Pathology
- Bone Metabolism and Diseases
- Photoreceptor and optogenetics research
- Zebrafish Biomedical Research Applications
- Medicinal Plant Pharmacodynamics Research
- Protein Kinase Regulation and GTPase Signaling
- Ion channel regulation and function
- Spinal Cord Injury Research
- Traumatic Brain Injury and Neurovascular Disturbances
- Cerebrospinal fluid and hydrocephalus
- RNA Research and Splicing
- Alzheimer's disease research and treatments
- Endoplasmic Reticulum Stress and Disease
- Barrier Structure and Function Studies
Catholic University of Korea
2014-2023
Kwangwoon University
2021
Catholic University of America
2015
University of Freiburg
1997-1998
Summary Auranofin (AF) is a sulphur‐containing gold compound. Because of its anti‐inflammatory and immunosuppressive activities, AF has been widely used for the therapeutic treatment rheumatoid arthritis. However, little known about mechanism action. To elucidate molecular underlying effect AF, we studied effects on cellular responses to interleukin‐6 (IL‐6). In HepG2 human hepatoma cells, markedly inhibited IL‐6‐induced phosphorylation janus kinase 1 (JAK1) signal transducer activator...
Abstract: There is increasing, although largely indirect, evidence that neurotrophic factors not only function as target‐derived survival for projection neurons, but also act locally to regulate developmental processes. We studied the expression of ciliary factor (CNTF) and CNTF‐specific ligand‐binding α‐subunit CNTF receptor complex (CNTFRα) in rat retina, a well‐defined CNS model system, effects on cultured retinal neurons. Both CNTFRα (mRNA protein) are expressed during phases...
α-Synuclein has been implicated in the pathogenesis of many neurodegenerative diseases, including Parkinson's disease and Alzheimer's disease. Although function α-synuclein remains largely unknown, recent studies have demonstrated that this protein can interact with phospholipids. To address role disease, we investigated whether it binds phospholipase D (PLD) affects PLD activity human embryonic kidney (HEK)-293 cells overexpressing wild type or mutant forms (A53T, A30P) associated Tyrosine...
Neurotrophic factors have been implicated in reactive processes occurring response to CNS lesions. Ciliary neurotrophic factor (CNTF), particular, has shown ameliorate axotomy-induced degeneration of neurons and be upregulated at wound sites the brain. To investigate a potential role CNTF lesion-induced reorganization, we analyzed expression protein receptor alpha (CNTFR alpha) mRNA rat dentate gyrus after unilateral entorhinal cortex lesions (ECLs), using immunocytochemistry nonradioactive...
Connexin 36 (Cx36) is a channel-forming protein found in the membranes of apposed cells, forming hexameric hemichannels intercellular gap junction channels. It localizes to certain neurons various regions brain including retina. We characterized expression pattern neuronal Cx36 guinea pig retina by immunocytochemistry using specific antisera against and green/red cone opsin or recoverin. Strong immunoreactivity was visible ON sublamina inner plexiform layer outer layer, as punctate labelling...
To determine whether the pathophysiological processes after transient forebrain ischemia are mediated via a signal pathway involving gp130 (a transducer for interleukin-6 family), we analyzed changes in expression of and its downstream transcription factor, activator factor 3 (STAT3), rat hippocampus four-vessel occlusive model. Expression mRNA was restricted to neurons pyramidal cell granule layers control animals. Four hours ischemic injury, astrocytes expressed mRNA. increased...
Osteopontin (OPN) is an adhesive glycoprotein linked to a variety of pathophysiological processes. We investigated whether OPN might act as opsonin in the diseased brain by studying postischemic expression and localization mRNA protein rat model ischemic stroke. In addition, we characterized subcellular core. Induction occurred activated microglia/macrophages core on days 3-7 after reperfusion this was sustained up day 28, at least. synthesized secreted macrophages, which first surrounded...
We have investigated the expression and cellular localization of ciliary neurotrophic factor (CNTF) in rat retina following ischemia induced by transiently increasing intraocular pressure. In normal retina, CNTF immunoreactivity was restricted to profiles ganglion cell layer. Following reperfusion, appeared Müller somata processes its intensity increased between 1 day 2 weeks post-lesion. Quantitative evaluation immunoblotting confirmed that continuously up after ischemic injury (to 600%...
Perivascular cells expressing platelet-derived growth factor receptor beta (PDGFR-β) have recently been implicated in fibrotic scar formation after acute brain injury, but their precise identity and detailed morphological characteristics remain elusive. This study sought to characterize define the cellular phenotype of vascular-associated PDGFR-β striatum rats treated with mitochondrial toxin 3-nitropropionic acid (3-NP). In control striatum, PDGFR-β-positive were invariably localized on...
Abstract Our aim was to examine the spatiotemporal profiles and phenotypic characteristics of neuron-glia antigen 2 (NG2) glia their associations with neuroglial cells in striatal lesions due mitochondrial toxin 3-nitropropionic acid (3-NP). In control striatum, weak NG2 immunoreactivity restricted resting thin processes, but prominent expression noted on activated microglia/macrophages, reactive lesion core after 3-NP injection. Activation glia, including enhanced proliferation...
Recent studies have suggested that nestin facilitates cellular structural remodeling in vasculature-associated cells response to ischemic injury. The current study was designed investigate the potential role of post-ischemic expression parenchymal astrocytes. With this aim, we characterized ischemia-induced CA1 hippocampal region, an area undergoes a delayed neuronal death, followed by lack generation after transient forebrain ischemia. Virtually all nestin-positive hippocampus were reactive...
Previous in vitro studies using cell cultures or brain slices have demonstrated that phospholipase D (PLD) the nervous system is involved signaling mechanism response to a variety of agonists. However, little known about pathophysiological role PLD-mediated adult brain. We examined changes expression PLD isozyme, PLD1, rat hippocampus, immunological approaches and an assay for activity after transient forebrain ischemia (four-vessel occlusion model) results selective delayed death CA1...
Immunohistochemistry for haptoglobin (Hp) in the postischemic hippocampus demonstrated an immunoreactivity visible one day after reperfusion and continuing to increase until 14 days ischemia. The was most prominent CA1 dentate hilar region, especially cells with astroglial morphology. Double immunofluorescence histochemistry confirmed colocalization of Hp glial fibrillary acidic protein. Furthermore, a reverse transcription-polymerase chain reaction study elevated mRNA level hippocampus....
Osteopontin (OPN, SPP1) is upregulated in response to acute brain injury, and based on its immunoreactivity, two distinct forms have been identified: intracellular OPN within macrophages small granular OPN, identified as OPN-coated degenerated neurites. This study investigates the spatiotemporal relationship between punctate deposition astroglial microglial reactions elicited by 3-nitropropionic acid (3-NP).Male Sprague-Dawley rats were intraperitoneally injected with mitochondrial toxin...