A5007544338- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- DNA Repair Mechanisms
- RNA modifications and cancer
- Histone Deacetylase Inhibitors Research
- Silk-based biomaterials and applications
- Blood groups and transfusion
- Platelet Disorders and Treatments
- CRISPR and Genetic Engineering
- Cancer-related gene regulation
- Zebrafish Biomedical Research Applications
- Silkworms and Sericulture Research
- Herpesvirus Infections and Treatments
- Cancer-related Molecular Pathways
- Neurobiology and Insect Physiology Research
- Virus-based gene therapy research
- Insect Resistance and Genetics
- Protein Degradation and Inhibitors
- RNA Research and Splicing
- Viral Infectious Diseases and Gene Expression in Insects
- Ubiquitin and proteasome pathways
- Blood disorders and treatments
- Cancer Genomics and Diagnostics
- Biochemical and Structural Characterization
- Congenital heart defects research
Inserm
2015-2023
Université de Montpellier
2008-2023
Centre National de la Recherche Scientifique
2002-2023
Institut de Recherche en Cancérologie de Montpellier
2015-2023
Université Gustave Eiffel
2023
Harvard University
2023
Université Clermont Auvergne
2022
Institut Regional du Cancer de Montpellier
2015-2020
BioCampus Montpellier
2018
Centre National pour la Recherche Scientifique et Technique (CNRST)
2018
PR-Set7/SET8 is a histone H4–lysine 20 methyltransferase required for normal cell proliferation. However, the exact functions of this enzyme remain to be determined. In study, we show that human PR-Set7 during S phase regulate cellular associates with replication foci and maintains bulk H4-K20 mono- trimethylation. Consistent function in chromosome dynamics phase, inhibition activity by small hairpin RNA causes replicative stress characterized alterations fork velocity origin firing. This...
Genetic toxicology studies are required for the safety assessment of chemicals. Data from these have historically been interpreted in a qualitative, dichotomous “yes” or “no” manner without analysis dose–response relationships. This article is based upon work an international multi-sector group that examined how quantitative relationships vitro and vivo genetic data might be used to improve human risk assessment. The three approaches analyzing curves deriving point-of-departure (POD) metrics...
In vertebrates, haematopoietic stem/progenitor cells (HSPCs) first emerge in the aorta–gonad–mesonephros (AGM) before colonizing transitory and subsequently definitive organs allowing haematopoiesis throughout adult life. Here we identify an unexpected primitive macrophage population accumulated dorsal mesenteric mesoderm surrounding aorta of human embryo study its function transparent zebrafish embryo. Our reveals dynamic interactions occurring between HSPCs macrophages AGM. Specific...
The decompaction and re-establishment of chromatin organization immediately after mitosis is essential for genome regulation. Mechanisms underlying structure control in daughter cells are not fully understood. Here we show that a compaction threshold exiting ensures integrity by limiting replication licensing G1 phase. Upon mitotic exit, relaxation controlled SET8-dependent methylation histone H4 on lysine 20. In the absence either SET8 or H4K20 residue, substantial genome-wide occurs...
Imprinted genes are important in development and their allelic expression is mediated by imprinting control regions (ICRs). On DNA‐methylated allele, ICRs marked trimethylation at H3 Lys 9 (H3K9me3) H4 20 (H4K20me3), similar to pericentric heterochromatin. Here, we investigate which histone methyltransferases this methylation of ICRs. We found that inactivation SUV4‐20H leads the loss H4K20me3 increased levels its substrate, H4K20me1. H4K20me1 controlled PR‐SET7 detected on both parental...
Highlights•Transcriptional target genes of E4F1 encode mitochondrial and checkpoint proteins•E4F1 controls the basal expression Chek1 gene•E4F1 transcriptional program impacts metabolism stress response•E4F1 is essential for cancer cell survival could be a therapiesSummaryRecent data support notion that group key regulators involved in tumorigenesis, including MYC, p53, E2F1, BMI1, share an intriguing capacity to simultaneously regulate cycle. Here, we show another factor, multifunctional...
DNA topoisomerase II (TOP2) plays a crucial role in DNA-associated processes by inducing transient double-strand breaks, making it an important target for DNA-damage stabilizing agents. Commonly used cancer therapy, these agents are designed to interfere with TOP2 cleavage complexes on chromatin. However, the epigenetic pathways influencing their effectiveness and resultant cellular responses remain elusive. Here, we combine vitro as well vivo genetic pharmacological approaches prostate...
BACKGROUND: Fetal or neonatal alloimmune thrombocytopenia (FMAIT) results from a maternal alloimmunization against fetal platelet (PLT) antigens. In Caucasian persons, HPA‐1a is the most frequently implicated antigen. During past few years, FMAIT has been reported associated with rare private STUDY DESIGN AND METHODS: Since first documented case of due to anti‐HPA‐9bw (Max ), no additional cases have reported. Here retrospective analysis presented referred our laboratories in recent years....
The frequency of human platelet antigen-1 (HPA-1) to HPA-11w (excluding HPA-8w) and HPA-15 systems was studied in four sub-Saharan populations: Beninese, Congolese (Democratic Republic Congo Kinshasa), Cameroonians, Aka pygmies (Central African Republic). No report HPA prevalence has previously been published concerning these populations which are characterized by the highest HPA-2b gene frequencies any reported date (Aka 0.393, Benin 0.292, Cameroon 0.237, 0.224) at lesser degree HPA-5b...
Nahuoic acids A-E (1-5) have been isolated from laboratory cultures of a Streptomyces sp. obtained tropical marine sediment. The structures the new polyketides 2-5 were elucidated by analysis spectroscopic data natural products and chemical derivatives 6 7. 1-5 are in vitro inhibitors histone methyltransferase SETD8, nahuoic acid A (1) its pentaacetate derivative 8 inhibit proliferation several cancer cells lines with modest potency. At IC50 for cell proliferation, showed selective...
Multiple myeloma (MM) is a malignancy of plasma cells that largely remains incurable. The search for new therapeutic targets therefore essential. In addition to wide panel genetic mutations, epigenetic alterations also appear as important players in the development this cancer, thereby offering possibility reveal novel approaches and effective intervention.Here, we show higher expression lysine methyltransferase SETD8, which responsible mono-methylation histone H4 at 20, an adverse prognosis...
Abstract: Several cytotoxic T lymphocyte peptide‐based vaccines against hepatitis B, human immunodeficiency virus and melanoma were recently studied in clinical trials. One interesting vaccine candidate alone or combination with other tumor antigens, is the decapeptide ELA. This peptide a Melan‐A/MART‐1 antigen immunodominant analog, an N‐terminal glutamic acid. It has been reported that amino group γ‐carboxylic of acids, as well γ‐carboxamide glutamines, condense easily to form pyroglutamic...
The human herpes simplex virus (HSV) host cell factor HCF-1 is a transcriptional coregulator that associates with both histone methyl- and acetyltransferases, deacetylase regulates proliferation division. In HSV-infected cells, the viral protein VP16 to promote formation of multiprotein-DNA activator complex. ability HCF proteins stabilize this VP16-induced complex has been conserved in diverse animal species including Drosophila melanogaster Caenorhabditis elegans suggesting targets...
The gene encoding the silk protein P25 in Bombyx mori is expressed posterior gland (PSG) cells and repressed middle (MSG) cells. To identify factors involved this transcription-dependent spatial restriction, we examined chromatin PSG MSG nuclei by DNase I-aided ligation-mediated PCR analyzed expression of various P25-lacZ constructs biolistically treated glands. promoter activation depends on two cis-acting elements. One coincides with target sequence SGFB, a gland-specific factor present...