A5043292394- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Herpesvirus Infections and Treatments
- Virus-based gene therapy research
- RNA modifications and cancer
- Glycosylation and Glycoproteins Research
- Toxin Mechanisms and Immunotoxins
- Cancer-related gene regulation
- Epigenetics and DNA Methylation
- RNA Interference and Gene Delivery
- Bacteriophages and microbial interactions
- interferon and immune responses
- Polyomavirus and related diseases
- Carbohydrate Chemistry and Synthesis
- DNA and Nucleic Acid Chemistry
- Plant Virus Research Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Liver physiology and pathology
- CRISPR and Genetic Engineering
- Ubiquitin and proteasome pathways
- Advanced biosensing and bioanalysis techniques
- Galectins and Cancer Biology
- Transgenic Plants and Applications
- Molecular Biology Techniques and Applications
University of Lausanne
2012-2023
Hudson Institute
2019
Merck Institute for Science Education
2019
John Wiley & Sons (United States)
2019
Cold Spring Harbor Laboratory
1997-2010
Harvard University
1981-2008
Massachusetts General Hospital
2008
Johannes Gutenberg University Mainz
2007
Fred Hutch Cancer Center
2003
State University of New York
1992-1999
The POU domain: a large conserved region in
R A Sturm, G Das, and W Herr Cold Spring Harbor Laboratory, New York 11724.
MLL (for mixed-lineage leukemia) is a proto-oncogene that mutated in variety of human leukemias. Its product, homolog Drosophila melanogaster trithorax, displays intrinsic histone methyltransferase activity and functions genetically to maintain embryonic Hox gene expression. Here we report the biochemical purification demonstrate it associates with cohort proteins shared yeast SET1 complexes, including Ash2, another Trx-G group protein. Two other members novel complex identified here are...
DNA-dependent and DNA-independent associations of DNA-binding proteins are important in transcriptional regulation. The analysis frequently relies on assaying protein interaction the absence target DNA sequences. We have found that contaminating preparations can stabilize may appear DNA-independent. Three cellular 70, 85, 110 kDa coimmunoprecipitated with octamer motif-binding Oct-2 because presence cell extracts. In addition, heterodimer formation between Oct-1 (or Oct-2) Pit-1 during...
The abundant and chromatin-associated protein HCF-1 is a critical player in mammalian cell proliferation as well herpes simplex virus (HSV) transcription. We show here that separate regions of for its role associate with the Sin3 histone deacetylase (HDAC) previously uncharacterized human trithorax-related Set1/Ash2 methyltransferase (HMT). HMT methylates H3 at Lys 4 (K4), but not if neighboring K9 residue already methylated. tethers transcriptional regulatory complexes together even though...
A secondary structure model for 23S ribosomal RNA has been constructed on the basis of comparative sequence data, including complete sequences from E. coli. Bacillus stearothermophilis , human and mouse mitochondria several partial sequences. The tested extensively with single strandspecific chemical enzymatic probes. Long range base-paired interactions organize molecule into six major structural domains containing over 100 individual helices in all. Regions sites interaction proteins 5S...
R Sturm, T Baumruker, B Franza, and W Herr Cold Spring Harbor Laboratory, New York 11724.
AKV is an endogenous, ecotropic murine leukemia virus that serves as one of the parents recombinant; oncogenic mink cell focus-forming viruses arise in preleukemic AKR mice. I report 8,374-nucleotide-long sequence AKV, determined from infectious molecular clone AKR-623. The 5'-leader extends to nucleotide 639, after which lies a long open reading frame encoding gag and pol gene products. interrupted by single amber codon separating genes. overlaps env within 3' region genome. 5' reveals...
Human HCF-1 is a large, highly conserved, and abundant nuclear protein that plays an important but unknown role in cell proliferation.It also activation of herpes simplex virus immediate-early gene transcription by the viral regulatory VP16.A single proline-to-serine substitution VP16 interaction domain causes temperature-induced arrest proliferation hamster tsBN67 cells prevents transcriptional VP16.We show here naturally bound to chromatin uninfected through its domain.HCF-1 at permissive...
Interactions of cell-autonomous circadian oscillators with diurnal cycles govern the temporal compartmentalization cell physiology in mammals. To understand transcriptional and epigenetic basis rhythms mouse liver genome-wide, we generated DNA occupancy profiles by RNA polymerase II (Pol II) as well histone modifications H3K4me3 H3K36me3. We used these data to quantify relationships phases amplitudes between different marks. found that rhythmic Pol recruitment at promoters rather than...
We have used the 100-kD HeLa cell octamer-binding protein OBP100 as a model to study flexible DNA sequence recognition by promoter-binding proteins. binds conserved octamer motif ATGCAAAT found in numerous promoters and additionally two degenerate motifs (sites I II) within SV40 enhancer region. show here that herpes simplex virus immediate early promoter TAATGARAT (R = purine) itself, extending flexibility of sequences bear very little resemblance (four matches over 14-bp region)....
Dual-Duty Active Site O-linked N-acetylglucosamine transferase (OGT) catalyzes the addition of (GlcNac) to serine or threonine residues, influencing localization and function proteins. Because its activity is sensitive nutrient uridine diphosphate (UDP)–GlcNac, OGT has been proposed regulate cellular responses status. Recently, in presence UDP-GlcNac was shown cleave host cell factor–1 (HCF-1), a transcriptional coregulator human cell-cycle progression. This cleavage required for HCF-1...