A5008492856- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Plant nutrient uptake and metabolism
- Chromosomal and Genetic Variations
- DNA Repair Mechanisms
- Genomics and Chromatin Dynamics
- Plant Reproductive Biology
- Plant-Microbe Interactions and Immunity
- Plant tissue culture and regeneration
- Plant Genetic and Mutation Studies
- Plant Stress Responses and Tolerance
- Plant Virus Research Studies
- Mitochondrial Function and Pathology
- Plant responses to water stress
- Plant Disease Resistance and Genetics
- Plant Gene Expression Analysis
- Legume Nitrogen Fixing Symbiosis
- CRISPR and Genetic Engineering
- Genomics and Phylogenetic Studies
- Cancer-related Molecular Pathways
- Protist diversity and phylogeny
- Nematode management and characterization studies
- Phytase and its Applications
- Light effects on plants
- Cocoa and Sweet Potato Agronomy
Institut des Sciences des Plantes de Paris Saclay
2016-2025
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2019-2025
Centre National de la Recherche Scientifique
2016-2025
Université Paris-Saclay
2016-2025
Université Paris Cité
2009-2024
Sorbonne Paris Cité
2016-2021
Université Paris-Sud
2009-2020
Sorbonne Université
2007-2018
Institut National de la Recherche Agronomique
2018
Institut des Sciences du Végétal
2005-2013
Methylations at position N6 of internal adenosines (m6As) are the most abundant and widespread mRNA modifications. These modifications play crucial roles in reproduction, growth, development by controlling gene expression patterns posttranscriptional level. Their function is decoded readers that share YTH domain, which forms a hydrophobic pocket directly accommodates m6A residues. While physiological molecular functions have been extensively studied animals, little known about plant readers,...
Abstract The complex and dynamic three-dimensional organization of chromatin within the nucleus makes understanding control gene expression challenging, but also opens up possible ways to epigenetically modulate expression. Because plants are sessile, they evolved sophisticated rapidly in response environmental stress, that thought be coordinated by changes conformation mediate specific cellular physiological responses. However, what extent how stress induces reorganization remains poorly...
Polyploidy is ubiquitous in eukaryotic plant and fungal lineages, it leads to the co-existence of several copies similar or related genomes one nucleus. In plants, polyploidy considered a major factor successful domestication. However, challenges chromosome folding architecture nucleus establish functional structures.
Precise expression patterns of genes in time and space are essential for proper development multicellular organisms. Dynamic chromatin conformation spatial organization the genome constitute a major step this regulation to modulate developmental outputs. Polycomb repressive complexes (PRCs) mediate stable or flexible gene repression response internal environmental cues. In Arabidopsis thaliana, LHP1 co-localizes with H3K27me3 epigenetic marks throughout interacts PRC1 PRC2 members as well...
In angiosperms, the gynoecium is last structure to develop within flower due determinate fate of floral meristem (FM) stem cells. The maintenance cell activity before its arrest at stage called FM termination affects number carpels that develop. necessary inhibition this WUSCHEL (WUS), which responsible for maintenance, involves a two-step mechanism. Direct repression mediated by MADS domain transcription factor AGAMOUS (AG), followed indirect requiring C2H2 zinc-finger protein KNUCKLES...
In animals, distant H3K27me3-marked Polycomb targets can establish physical interactions forming repressive chromatin hubs. plants, growing evidence suggests that H3K27me3 acts directly or indirectly to regulate interactions, although how this histone modification modulates 3D architecture remains elusive. To decipher the impact of dynamic deposition on Arabidopsis thaliana nuclear interactome, we combined genetics, transcriptomics, and several epigenomic approaches. By analyzing mutants...
Although it is a crucial cellular process required for both normal development and to face stress conditions, the control of programmed cell death in plants not fully understood. We previously reported isolation ATXR5 ATXR6, two PCNA-binding proteins that could be involved regulation cycle or death. A yeast two-hybrid screen using as bait captured AtIPS1, an enzyme which catalyses committed step myo-inositol (MI) biosynthesis. atips1 mutants form spontaneous lesions on leaves, raising...
SWI/SNF complexes mediate ATP-dependent chromatin remodeling to regulate gene expression. Many components of these are evolutionarily conserved, and several subunits Arabidopsis thaliana involved in the control flowering, a process that depends on floral repressor FLOWERING LOCUS C (FLC). BAF60 is subunit, this work, we show BAF60, via direct targeting FLC, induces change at high-order level represses photoperiod flowering pathway Arabidopsis. accumulates nucleus controls formation FLC loop...
Microbial-associated molecular patterns activate several MAP kinases, which are major regulators of the innate immune response in Arabidopsis thaliana that induce large-scale changes gene expression. Here, we determine whether microbial-associated pattern-triggered expression involves modifications at chromatin level. Histone acetylation and deacetylation implicate histone deacetylase HD2B reprogramming defence immunity. The kinase MPK3 directly interacts with phosphorylates HD2B, thereby...
Polycomb repressive complexes (PRCs) have been traditionally associated with the regulation of developmental processes in various organisms, including higher plants. However, similar to other epigenetic regulators, there is accumulating evidence for their role stress and immune-related pathways. In current study we show that PRC1 protein LHP1 required repression MYC2 branch jasmonic acid (JA)/ethylene (ET) pathway immunity. Loss induces reduction H3K27me3 levels gene bodies ANAC019 ANAC055,...
Abstract The modification of histones by acetyl groups has a key role in the regulation chromatin structure and transcription. Arabidopsis thaliana histone acetyltransferase GCN5 regulates modifications as part Spt-Ada-Gcn5 Acetyltransferase (SAGA) transcriptional coactivator complex. was previously shown to acetylate lysine 14 3 (H3K14ac) promoter regions its target genes even though binding did not systematically correlate with gene activation. Here, we explored mechanism through which...
Histone modifications deposited by the Polycomb repressive complex 2 (PRC2) play a critical role in control of growth, development, and adaptation to environmental fluctuations most multicellular eukaryotes. The catalytic activity PRC2 is counteracted Jumonji-type (JMJ) histone demethylases, which shapes genomic distribution H3K27me3. Here, we show that two JMJ demethylases Arabidopsis , EARLY FLOWERING 6 (ELF6) RELATIVE OF (REF6), distinct roles H3K27me3 H3K27me1 homeostasis. We failure...
How cell size and number are determined during organ development remains a fundamental question in biology. Here, we identified GRAS family transcription factor, called SCARECROW-LIKE28 (SCL28), with critical role determining Arabidopsis. SCL28 is part of transcriptional regulatory network downstream the central MYB3Rs that regulate G2 to M phase cycle transition. We show forms dimer AP2-type AtSMOS1, which defines specificity for promoter binding directly activates specific set...
The proliferating cell nuclear antigen (PCNA) functions as a sliding clamp for DNA polymerase, and is thus key actor in replication. It also involved repair, maintenance of heterochromatic regions throughout replication, cycle regulation programmed death. Identification PCNA partners therefore necessary understanding these processes. Here we identify two Arabidopsis SET-domain proteins that interact with PCNA: ATXR5 ATXR6. A truncated ATXR5Deltaex2, incapable interacting PCNA, occurs planta....
A salient feature of organelle gene expression is the requirement for nucleus-encoded factors that act posttranscriptionally in a gene-specific manner. central issue to understand whether these are merely constitutive or have regulatory function. In unicellular alga Chlamydomonas reinhardtii, chloroplast petA gene-encoding cytochrome f, major subunit b(6)f complex, depends on two specific factors: MCA1, required stable accumulation transcript, and TCA1, its translation. We cloned TCA1 gene,...
Abstract Organelle gene expression is characterized by nucleus-encoded trans-acting factors that control posttranscriptional steps in a gene-specific manner. As typical example, Chlamydomonas reinhardtii, of the chloroplast petA encoding cytochrome f, major subunit b 6 f complex, depends on MCA1 and TCA1, required for accumulation translation mRNA. Here, we show these two proteins associate high molecular mass complexes also contain We demonstrate degraded upon interaction with unassembled...
Abstract Programmed cell death (PCD) is essential for several aspects of plant life, including development and stress responses. Indeed, incompatible plant-pathogen interactions are well known to induce the hypersensitive response, a localized death. Mutational analyses have identified key PCD components, we recently mips1 mutant Arabidopsis (Arabidopsis thaliana), which deficient enzyme catalyzing limiting step myoinositol synthesis. One most striking features light-dependent formation...
Plant adaptive responses to changing environments involve complex molecular interplays between intrinsic and external signals. Whilst much is known on the signaling components mediating diurnal, light, temperature controls plant development, their influence chromatin-based transcriptional remains poorly explored. In this study we show that a SWI/SNF chromatin remodeler subunit, BAF60, represses seedling growth by modulating DNA accessibility of hypocotyl cell size regulatory genes. BAF60...
The cell division cycle involves nuclear and cytoplasmic events, namely organelle multiplication distribution between the daughter cells. Until now, plastid plant have been considered as independent processes because they can be uncoupled. Here, down-regulation of AtCDT1a AtCDT1b, members prereplication complex, is shown to alter both DNA replication in Arabidopsis thaliana . These data constitute molecular evidence for relationships cell-cycle division. Moreover, severe developmental...
* Minichromosome maintenance (MCM) proteins are subunits of the pre-replication complex that probably function as DNA helicases during S phase cell cycle. Here, we investigated AtMCM2 in Arabidopsis. To gain an insight into AtMCM2, combined loss- and gain-of-function approaches. this end, analysed two null alleles generated transgenic plants expressing downstream constitutive 35S promoter. Disruption is lethal at a very early stage embryogenesis, whereas its over-expression results reduced...