Abstract Global warming has become a critical challenge to food security, causing severe yield losses of major crops worldwide. Conventional and transgenic breeding strategies enhance plant thermotolerance are laborious expensive. Therefore, the use beneficial microbes could be an alternative approach. Here, we report that root endophyte Enterobacter sp. SA187 induces in wheat laboratory as well open‐field agriculture. To unravel molecular mechanisms, used Arabidopsis thaliana model plant....

Precise expression patterns of genes in time and space are essential for proper development multicellular organisms. Dynamic chromatin conformation spatial organization the genome constitute a major step this regulation to modulate developmental outputs. Polycomb repressive complexes (PRCs) mediate stable or flexible gene repression response internal environmental cues. In Arabidopsis thaliana, LHP1 co-localizes with H3K27me3 epigenetic marks throughout interacts PRC1 PRC2 members as well...

Several plant species require microbial associations for survival under different biotic and abiotic stresses. In this study, we show that Enterobacter sp. SA187, a desert endophytic bacterium, enhances yield of the crop alfalfa field conditions as well growth model Arabidopsis thaliana in vitro, revealing high potential SA187 biological solution improving production. Studying interaction with Arabidopsis, uncovered number mechanisms related to beneficial association plants. colonizes both...

Significance Although plant growth–promoting bacteria (PGPB) enhance the performance of plants, only a few mechanisms have been identified so far. We show that sulfur metabolisms in both PGPB Enterobacter sp. SA187 and Arabidopsis plants play key role salt stress tolerance. Salt induces starvation response is attenuated by SA187. metabolic mutants are hypersensitive to but can be rescued Most metabolism occurs chloroplasts linked stress-induced accumulation reactive oxygen species suppressed...

Alternative splicing (AS) of pre-mRNAs in plants is an important mechanism gene regulation environmental stress tolerance but plant signals involved are essentially unknown. Pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) mediated by mitogen-activated protein kinases and the majority PTI defense genes regulated MPK3, MPK4 MPK6. These responses have been mainly analyzed at transcriptional level, however many factors direct targets MAPKs. Here, we studied alternative...

Linker H1 histones play an important role in animal and human pathogenesis, but their function plant immunity is poorly understood. Here, we analyzed mutants of the three canonical variants Arabidopsis histones, namely H1.1, H1.2 H1.3. We observed that double h1.1h1.2 triple h1.1h1.2h1.3 (3h1) were resistant to Pseudomonas syringae Botrytis cinerea infections. Transcriptome analysis 3h1 mutant plants showed H1s a key regulating expression early late defense genes upon pathogen challenge....

Pectin is a major component of primary cell wall all land plants and encompasses range galacturonic acid rich polysaccharides.Pectinase or pectinolytic enzyme, hydrolyzes pectic substance, they have share 25% in the global sales food enzymes.Production this enzyme affected by nature solid substrate, level moisture content, presence absence carbon, nitrogen, minerals vitamin supplements.Maximum production (44U/ml) achieved when temperature around 37°C, pH.8.5, pectin as carbon source, yeast...

The Trihelix Transcription factor GT2-like 1 (GTL1) was previously shown to be a key regulator of ploidy-dependent trichome growth and drought tolerance. Here, we report that GTL1 plays an important role in coordinating plant immunity. We show gtl1 mutants are compromised the regulation basal immunity, microbial pattern-triggered immunity (PTI) effector-triggered RIN4-mediated Transcriptome analysis revealed positively regulates defense genes inhibits factors mediate development. By...

Significance Mitogen-activated protein kinases (MAPKs) function in all eukaryotes signaling extracellular stimuli to intracellular responses and ultimately link them chromatin events by targeting transcription factors remodeling complexes. In plants, MAPKs play crucial roles immunity, development, stress responses, but so far no attempts have been made identify phosphorylation of chromatin-associated proteins. By using a phosphoproteomic approach on MAPK mutants, we identified number...

The INDETERMINATE DOMAIN (IDD) family belongs to a group of plant-specific transcription factors that coordinates plant growth/development and immunity. However, the function mode action IDDs during abiotic stress, such as salt, are poorly understood. We used idd4 transgenic lines screened them under salt stress find involvement IDD4 in salinity tolerance genetic disruption increases salt-tolerance, characterized by sustained growth, improved Na+/K+ ratio, decreased stomatal...

Constitutive and alternative splicing of pre-mRNAs from multiexonic genes controls the diversity proteome; these precisely regulated processes also fine-tune responses to cues related growth, development, stresses. Small-molecule inhibitors that perturb provide invaluable tools for use as chemical probes uncover molecular underpinnings regulation potential anticancer compounds. Here, we show herboxidiene (GEX1A) inhibits both constitutive splicing. Moreover, GEX1A activates genome-wide...

Melon (Cucumis melo) is an important vegetable crop from the Cucurbitaceae family and a reference model specie for sex determination, fruit ripening vascular fluxes studies. Nevertheless, nature role of its epigenome in gene expression regulation more specifically determination remains largely unknown. We have investigated genome wide H3K27me3 H3K9ac histone modifications dynamics, five melon organs. were mainly distributed along gene-rich regions constrained to bodies. was preferentially...

Climate change and ocean warming threaten the persistence of corals worldwide. Genomic resources are critical to study evolutionary trajectory, adaptive potential, genetic distinctiveness coral species. Here, we provide a reference genome cauliflower Pocillopora verrucosa, broadly prevalent reef-building with important ecological roles in maintenance reefs across Red Sea, Indian Ocean, Pacific Ocean. The has an assembly size 380,505,698 bp scaffold N50 333,696 contig 75,704 bp. annotation...

Pathogen-associated molecular pattern (PAMP) recognition occurs by plasma membrane located receptors that induce among other processes nuclear gene expression. However, signaling to the compartment is restricted envelope and pore complexes. We show here four Arabidopsis lamin homologs LITTLE NUCLEI/CROWDED NUCLEI (LINC/CRWN), LINC1 plays an important role in PTI jasmonic acid (JA) signaling. linc1 knock out mutants affect PAMP-triggered MAPK activation growth inhibition, but not reactive...

INDETERMINATE DOMAIN (IDD)/ BIRD proteins are a highly conserved plant-specific family of transcription factors which play multiple roles in plant development and physiology. Here, we show that mutation IDD4/IMPERIAL EAGLE increases resistance to the hemi-biotrophic pathogen Pseudomonas syringae, indicating IDD4 may act as repressor basal immune response PAMP-triggered immunity. Furthermore, idd4 mutant exhibits enhanced plant-growth suppressor growth development. Transcriptome comparison...

The advent of high throughput sequencing technologies provides an opportunity to resolve phylogenetic relationships among closely related species. By incorporating hundreds thousands unlinked loci and single nucleotide polymorphisms (SNPs), phylogenomic analyses have a far greater potential species boundaries than approaches that rely on only few markers. Scleractinian taxa proved challenging identify using traditional morphological many groups lack adequate set molecular markers investigate...

INDETERMINATE DOMAIN (IDD)/BIRD proteins belong to a highly conserved plant-specific group of transcription factors with dedicated functions in plant physiology and development. Here, we took advantage the chimeric repressor gene-silencing technology (CRES-T, SRDX) widen our view on role IDD4/IMPERIAL EAGLE IDD family members immunity. The hypomorphic idd4SRDX lines are compromised growth show robust autoimmune phenotype. Hormonal measurements revealed concomitant accumulation salicylic acid...

Mammalian Ras-GTPase–activating protein SH3-domain–binding proteins (G3BPs) are a highly conserved family of RNA-binding that link kinase receptor-mediated signaling to RNA metabolism. G3BP1 is multifunctional functions in viral immunity. Here, we show the Arabidopsis thaliana homolog human negatively regulates plant g3bp1 mutants showed enhanced resistance virulent bacterial pathogen Pseudomonas syringae pv. tomato . Pathogen was mediated Atg3bp1 by altered stomatal and apoplastic...

Abstract Biogenesis of ribonucleoproteins occurs in dynamic subnuclear compartments called Cajal bodies (CBs). COILIN is a critical scaffolding component essential for CB formation, composition, and activity. We recently showed that Arabidopsis (Arabidopsis thaliana) AtCOILIN phosphorylated response to bacterial elicitor treatment. Here, we further investigated the role plant innate immunity. Atcoilin mutants are compromised defense responses pathogens. Besides confirming alternative...

Abstract Background The symbiotic relationship between cnidarians and dinoflagellates is one of the most widespread endosymbiosis in our oceans provides ecological basis coral reef ecosystems. Although many studies have been undertaken to unravel molecular mechanisms underlying these symbioses, we still know little about epigenetic that control transcriptional responses symbiosis. Results Here, used model organism Exaiptasia diaphana study genome-wide patterns putative functions histone...

Summary Root endophytes establish beneficial interactions with plants, improving holobiont resilience and fitness, but how plant immunity accommodates microbes is poorly understood. The multi‐stress tolerance‐inducing endophyte Enterobacter sp. SA187 triggers a canonical immune response in Arabidopsis only at high bacterial dosage (>10 8 CFUs ml −1 ), suggesting that able to evade or suppress the defence system lower titres. Although flagellin epitopes are recognized by FLS2 receptor,...

Abstract Global warming has become a critical challenge to food safety, causing severe yield losses of major crops worldwide. Heat acclimation empowers plants survive under extreme temperature conditions but the potential beneficial microbes make thermotolerant not been considered so far. Here, we report that endophytic bacterium Enterobacter sp. SA187 induces heat tolerance in Arabidopsis thaliana by reprogramming plant transcriptome similar extent as acclimation. Acclimation priming stress...