A5000771078- Plant-Microbe Interactions and Immunity
- Plant Stress Responses and Tolerance
- Legume Nitrogen Fixing Symbiosis
- CRISPR and Genetic Engineering
- Plant tissue culture and regeneration
- RNA Research and Splicing
- Plant Virus Research Studies
- Plant Parasitism and Resistance
- Plant Gene Expression Analysis
- Plant Molecular Biology Research
- Moringa oleifera research and applications
- Microbial Community Ecology and Physiology
- Insect symbiosis and bacterial influences
- Photosynthetic Processes and Mechanisms
- Biofuel production and bioconversion
- Seed and Plant Biochemistry
- Plant Pathogenic Bacteria Studies
- Antibiotic Resistance in Bacteria
- Pharmaceutical and Antibiotic Environmental Impacts
- Biocrusts and Microbial Ecology
- Identification and Quantification in Food
- Agricultural Productivity and Crop Improvement
- Plant Micronutrient Interactions and Effects
- Advances in Cucurbitaceae Research
- Bacteriophages and microbial interactions
King Abdulaziz University
2018-2025
Ain Shams University
2020
King Abdullah University of Science and Technology
2014-2018
The CRISPR/Cas9 system provides bacteria and archaea with molecular immunity against invading phages conjugative plasmids. Recently, has been used for targeted genome editing in diverse eukaryotic species.In this study, we investigate whether the could be plants to confer DNA viruses. We deliver sgRNAs specific coding non-coding sequences of tomato yellow leaf curl virus (TYLCV) into Nicotiana benthamiana stably overexpressing Cas9 endonuclease, subsequently challenge these TYLCV. Our data...
Targeted genomic regulation is a powerful approach to accelerate trait discovery and development in agricultural biotechnology. Bacteria archaea use clustered regularly interspaced short palindromic repeats (CRISPRs) CRISPR-associated (Cas) regulatory systems for adaptive molecular immunity against foreign nucleic acids introduced by invading phages conjugative plasmids. The type II CRISPR/Cas system has been adapted genome editing many cell types organisms. A recent study used the...
Plant trait engineering requires efficient targeted genome-editing technologies. Clustered regularly interspaced palindromic repeats (CRISPRs)/ CRISPR associated (Cas) type II system is used for applications across eukaryotic species including plants. Delivery of genome reagents and recovery mutants remain challenging tasks in planta applications. Recently, we reported the development Tobacco rattle virus (TRV)-mediated editing Nicotiana benthamiana. TRV infects growing points possesses...
The operative mechanisms and advantageous synergies existing between the rhizobiome wild plant species Abutilon fruticosum were studied. Within purview of this scientific study, reservoir genes in rhizobiome, encoding most highly enriched enzymes, was dominantly constituted by members phylum Thaumarchaeota within archaeal kingdom, Proteobacteria bacterial Streptophyta eukaryotic kingdom. ensemble enzymes encoded through exudation exhibited affiliations with 15 crosstalking KEGG (Kyoto...
The effects of three rhizobacterial isolates namely Pseudomonas fluorescens (M1), putida (M2) and Bacillus subtilis (M3) were examined to enhance growth chemical components such as chlorophyll proline cultivars soybean (Glycine max L.) under two levels salinity stress (S1 = 200 mM S2 400 NaCl salt). Several morphological physiological parameters investigated. highest mean values final germination percent (FGP) registered in cultivar Crawford (95%) followed by Giza111 (93%) the presence P....
Plants employ diverse strategies to cope with different types of heat stress. The response short-term acute stress differs significantly from that moderate followed by severe events. After experiencing stress, plants exhibit a more robust subsequent phenomenon known as thermopriming or acquired thermotolerance. Thermopriming creates memory maintaining the (HS) memory-related genes in an alert state. In this work, we investigated role Arabidopsis Universal Stress Protein 1 (USP1) plant...
Catharanthus roseus has various terpenoid indole alkaloids (TIAs) with adaptive mechanisms to withstand both biotic and abiotic stresses. We investigated the effects of methyl jasmonate (MeJA) on alternative splicing (AS) in C. identify differentially expressed alternatively spliced (DAS) genes following MeJA treatment. found pairs co-expressed factors (SFs) DAS potential roles SFs maturation their respective transcripts. Twenty two clusters encompassing 17 MeJA-responsive 10 SF genes....
Significance Mitogen-activated protein kinases (MAPKs) function in all eukaryotes signaling extracellular stimuli to intracellular responses and ultimately link them chromatin events by targeting transcription factors remodeling complexes. In plants, MAPKs play crucial roles immunity, development, stress responses, but so far no attempts have been made identify phosphorylation of chromatin-associated proteins. By using a phosphoproteomic approach on MAPK mutants, we identified number...
The study aims to describe phageome of soil rhizosphere M.oleifera in terms the genes encoding CAZymes and other KEGG enzymes.Genes rhizospheric virome wild plant species Moringa oleifera were investigated for their ability encode useful (Kyoto Encyclopedia Genes Genomes) enzymes resist antibiotic resistance (ARGs) soil.Abundance these was higher microbiome than bulk soil. Detected viral families include family Potyviridae as well tailed bacteriophages class Caudoviricetes that are mainly...
The current investigation endeavors to identify differentially expressed alternatively spliced (DAS) genes that exhibit concordant expression with splicing factors (SFs) under diverse multifactorial abiotic stress combinations in Arabidopsis seedlings. SFs serve as the post-transcriptional mechanism governing spatiotemporal dynamics of gene expression. different stresses encompass variations salt concentration, heat, intensive light, and their combinations. Clusters demonstrating consistent...
The metagenomic whole genome shotgun sequencing (mWGS) approach was used to detect signatures of the rhizosphere microbiomes Dipterygium glaucum and surrounding bulk soil microbiomes, differential microbial responses due watering. Preliminary results reflect reliability experiment rationality grouping microbiomes. Based on abundance non-redundant genes, bacterial genomes showed highest level, followed by Archaeal Eukaryotic genomes, then, least abundant viruses. Overall indicate that most...
Mammalian Ras-GTPase–activating protein SH3-domain–binding proteins (G3BPs) are a highly conserved family of RNA-binding that link kinase receptor-mediated signaling to RNA metabolism. G3BP1 is multifunctional functions in viral immunity. Here, we show the Arabidopsis thaliana homolog human negatively regulates plant g3bp1 mutants showed enhanced resistance virulent bacterial pathogen Pseudomonas syringae pv. tomato . Pathogen was mediated Atg3bp1 by altered stomatal and apoplastic...
The present study aims at the use of transcriptomic data wild barley (Hordeum spontaneum) under salt stress in detecting regulated metabolites and crosstalking signal transduction pathways that help plants withstand harsh conditions. studied involved those emphasizing roles phytohormones auxin abscisic acid (ABA) conferring tolerance adaptation. Of which, tryptophan-core include trigger auxin-responsive genes towards downstream cascades better plant growth. Analysis 'MAPK signalling' pathway...
The functional processes and mutual benefits of the wild plant Moringa oleifera its rhizosphere microbiome were studied via metagenomic whole-genome shotgun sequencing (mWGS) in comparison with a bulk soil microbiome. results indicated high gene abundance four KEGG categories, “Cellular Processes”, “Environmental Information Processing”, “Genetic “Metabolism”, Most enriched enzymes rhizobacteria are assigned to pathway “Amino acids metabolism”, where soil-dwelling microbes use amino as...
This study was carried out to determine the consequence of foliar application agmatine (0 and 0.5 mM), on growth, physiological biochemical traits, yield turnip (Brassica rapa L.) plants grown under water stress or salt conditions. The effect three irrigation regimes (100%, 80% 40% field capacity) concentrations (0, 100 200 mM NaCl) in pots greenhouse conditions were studied. Water deficit developed at Field capacity (FC) salinity stress, especially mM, resulted significant decreases all...
Abstract Biogenesis of ribonucleoproteins occurs in dynamic subnuclear compartments called Cajal bodies (CBs). COILIN is a critical scaffolding component essential for CB formation, composition, and activity. We recently showed that Arabidopsis (Arabidopsis thaliana) AtCOILIN phosphorylated response to bacterial elicitor treatment. Here, we further investigated the role plant innate immunity. Atcoilin mutants are compromised defense responses pathogens. Besides confirming alternative...
Moringa oleifera (or the miracle tree) is a wild plant species widely grown for its seed pods and leaves, used in traditional herbal medicine. The metagenomic whole genome shotgun sequencing (mWGS) approach was to characterize antibiotic resistance genes (ARGs) of rhizobiomes this surrounding bulk soil microbiomes figure out chance consequences highly abundant ARGs, e.g., mtrA , golS soxR oleC novA kdpE vanRO parY rbpA horizontally transfer human gut pathogens via mobile genetic elements...
The challenge of climate change makes it mandatory to improve tolerance drought stress in bread wheat (Triticum aestivum) via biotechnological approaches. Drought experiment was conducted followed by RNA-Seq analysis for leaves two cultivars namely Giza 168 and Gemmiza 10 with contrasting genotypes. Expression patterns the regulated stress-related genes concordantly expressed TFs were detected, then, validated qPCR loss-of-function mutants Arabidopsis background harboring mutated analogue...
The coast of the Red Sea in Jeddah City is home to a unique microbial community that has adapted extreme environmental conditions. Therefore, it essential characterize this microbiome predict how changes will affect it. aim study was conduct metagenomic sequencing 16S rRNA and ITS genes for taxonomic classification soil samples associated with halophytic plants Tamarix aphylla Halopeplis perfoliata. Fifteen were collected triplicate enhance robustness minimize sampling bias. Firstly,...