- Plant responses to elevated CO2
- Plant Water Relations and Carbon Dynamics
- Photosynthetic Processes and Mechanisms
- Plant nutrient uptake and metabolism
- Weed Control and Herbicide Applications
- Soil Carbon and Nitrogen Dynamics
- Legume Nitrogen Fixing Symbiosis
- Bioenergy crop production and management
- Amino Acid Enzymes and Metabolism
- Isotope Analysis in Ecology
- Rangeland and Wildlife Management
- Plant Pathogens and Resistance
- Atmospheric and Environmental Gas Dynamics
- Biocrusts and Microbial Ecology
- Peatlands and Wetlands Ecology
- Aquatic Ecosystems and Phytoplankton Dynamics
- Plant Stress Responses and Tolerance
- Algal biology and biofuel production
- Geology and Paleoclimatology Research
- Plant Ecology and Soil Science
- Agriculture and Biological Studies
- Soil erosion and sediment transport
- Coenzyme Q10 studies and effects
- Microbial Metabolites in Food Biotechnology
- Mitochondrial Function and Pathology
Institut Supérieur d'Agriculture Rhône-Alpes
2022-2024
Agroécologie
2022-2024
Australian National University
2013-2020
Institut des Sciences des Plantes de Paris Saclay
2009-2013
Université Paris-Sud
2007-2013
Centre National de la Recherche Scientifique
2007-2013
Écologie, Systématique et Évolution
2008-2009
Molina Center for Energy and the Environment
2009
Université Paris-Saclay
2009
Weizmann Institute of Science
2009
DOI: 10.1111/j.1469-8137.2007.02336.x
Abstract Starch and soluble sugars are the major photosynthetic products, their carbon isotope signatures reflect external versus internal limitations of CO 2 fixation. There has been recent renewed interest in composition carbohydrates, mainly for use flux partitioning studies at ecosystem level. The obstacle to carbohydrates such lack an acknowledged method isolate starch isotopic measurements. We here report on comparison evaluation existing methods (acid enzymatic hydrolysis starch;...
   In the short- or mid-term, variation of leaf-respired δ13CO2 has important consequences for δ13C CO2 in air terrestrial ecosystems. Therefore, isotope composition plant respired is crucial importance understanding and ecosystem carbon balance. It previously been shown tobacco (Nicotiana tabacum) that balance between ammonium nitrate an influence on CO2. However, uncertainty remains as to whether (i) effect N nutrition observed all species, (ii) source...
DOI: 10.1111/j.1469-8137.2008.02719.x Commentary p 243
Ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) is the cornerstone of atmospheric CO
Summary In gas‐exchange experiments, manipulating CO 2 and O is commonly used to change the balance between carboxylation oxygenation. Downstream metabolism (utilization of photosynthetic photorespiratory products) may also be affected by gaseous conditions but this not well documented. Here, we took advantage sunflower as a model species, which accumulates chlorogenate in addition sugars amino acids (glutamate, alanine, glycine serine). We performed isotopic labelling with 13 under...
Abstract The study presents a comparison of two phloem sugar extraction methods. amount extracted and the carbon isotope composition ( δ 13 C) total extracts main compounds separated by high‐performance liquid chromatography (sucrose, glucose, fructose pinitol) are compared. These sap methods exudation in distilled water new method using centrifugation, which avoids addition any solvent. We applied both on discs sampled from 38‐year‐old Pinus pinaster trees south‐western France throughout...
Abstract The natural 13 C/ 12 C isotope composition ( δ C) of plants and organic compounds within plant organs is a powerful tool to understand carbon allocation patterns the regulation photosynthetic or respiratory metabolism. However, many enzymatic fractionations are currently unknown, thus impeding our understanding trafficking pathways cells. One them effect associated with invertases (EC 3.2.1.26) that cornerstone enzymes for Suc metabolism translocation in plants. Another conundrum...
d-Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) is the most abundant enzyme on Earth and responsible for fixation of atmospheric CO(2) into biomass. The reaction consists incorporation solvent H(2)O d-ribulose 1,5-bisphosphate (RuBP) to yield 3-phospho-d-glycerate. involves several proton-dependent events: abstraction protonation during enolization RuBP hydrolysis reprotonation six-carbon intermediate (carboxyketone). Although much known about Rubisco structure diversity,...
The flux (R(s)) and carbon isotopic composition (delta(13)C (Rs)) of soil respired CO (2) was measured every 2 h over the course three diel cycles in a Mediterranean oak woodland, together with measurements delta(13)C leaf, root organic matter (SOM)) metabolites. Simulations R(s) (Rs) were also made using numerical model parameterised SOM data assuming short-term production rates driven mainly by temperature. Average values study period within range metabolite average (SOM) values, but...
Abstract The response of root metabolism to variations in carbon source availability is critical for whole‐plant nitrogen (N) assimilation and growth. However, the effect changes carbohydrate input intact roots currently not well understood and, example, both smaller larger values root:shoot ratios or N uptake have been observed so far under elevated CO 2 . In addition, previous studies on sugar starvation mainly focused senescent excised organs while an increasing body data suggests that...
Abstract The δ 13 C (carbon isotope composition) variations in respired CO 2 , total organic matter, proteins, sucrose and starch have been measured during tuber sprouting of potato ( Solanum tuberosum ) darkness. Measurements were carried out both on tubers their growing sprouts for 23 days after the start sprout development. Sucrose was slightly C‐depleted compared with tubers, suggesting that breakdown associated a small fractionation. In sprouts, all biochemical fractions including...
Nitrogen (N) nutrition impacts on primary carbon metabolism and can lead to changes in δ
Abstract The perennial grain intermediate wheatgrass ( Thinopyrum intermedium , commercial name Kernza TM ) has been proposed as a diversification crop for producing forage and providing ecosystem services to farmers. Although few studies have addressed farmers’ interests in the crop, information is lacking about links between goals management, i.e., how farmers aim at integrating this their systems. Closing gap, paper analyzes first time introduction of (IWG) from farmer perspective, set...
Despite considerable advances in the past 50 y, mechanism of ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) catalysis is still not well understood. In particular, movement and exchange protons within active site documented: typically, kinetics H during first steps catalysis, i.e. abstraction H3 atom (RuBP) enolization, are clearly established. Here, we took advantage reaction assays run heavy water (2H2O) to monitor appearance deuterated RuBP products (3-phosphoglycerate...
The development of a new perennial grain offers opportunities to diversify annual crop rotations which are being singled out as negative contributors soil and resource conservation compared stands in natural ecosystems. transition from cropping systems solely based on annuals, mixing both annuals perennials, is expected reduce the need weed management through increased control capacity perennials. However, potential effectively suppress weeds remains mainly theoretical (based previous...