The nucleotide sequence of the coding and regulatory regions alpha-amylase gene (aml) Streptomyces limosus was determined. High-resolution S1 mapping used to locate 5' end transcript demonstrated that is transcribed from a unique promoter. predicted amino acid has considerable identity mammalian invertebrate alpha-amylases, but not those plant, fungal, or eubacterial origin. Consistent with this susceptibility enzyme an inhibitor alpha-amylases. amino-terminal extracellular determined,...

An atomic force microscope coupled with a tunable infrared laser source (AFM-IR) was used to measure the size and map distribution of oil inclusions inside microorganism without staining or other special sample preparation. The under study is Streptomyces, soil bacterium that possesses capability, some specific nutritional conditions, store its carbon into TriAcylGlycerols, potential direct biodiesel.

The Streptomyces genus is well known for its ability to produce bio-active secondary metabolites of great medical interest. However, the metabolic features accompanying these bio-productions remain be defined. In this study, comparison related model strains producing differing levels actinorhoddin (ACT), showed that S. lividans, a weak producer, had high TriAcylGlycerol (TAG) content indicative glycolytic metabolism. contrast, strong coelicolor, was characterized by low TAG content, active...

The PhoR/PhoP two-component system of Streptomyces lividans was previously shown to allow the growth bacteria at low Pi concentrations and negatively control antibiotic production. present study focuses on transcriptional analysis phoR phoP, along with phoU mtpA genes that are transcribed divergently from phoRP operon in S. lividans. effect phoR, phoU, ppk mutations transcription these examined under phosphate-replete phosphate-limited conditions. We demonstrated phoP were cotranscribed as a...

An alpha-amylase gene (aml) of Streptomyces limosus ATCC 19778 was cloned in lividans 66. S1 mapping experiments identified an aml transcript 1.8 kb length and the extracellular enzyme estimated to be 59 kD size, suggesting that transcribed as a monocistronic mRNA species. Expression induced by maltose (or maltodextrins) S. when or coelicolor A3(2). In limosus, mannitol repressed expression while glucose had little no effect; relative effects two sugars were reversed. Both induction...

The ppk gene of Streptomyces lividans encodes an enzyme catalyzing, in vitro, the reversible polymerization gamma phosphate ATP into polyphosphate and was previously shown to play a negative role control antibiotic biosynthesis (H. Chouayekh M. J. Virolle, Mol. Microbiol. 43:919-930, 2002). In present work, some regulatory features expression were established content S. TK24 mutant determined. Pi sufficiency, be low but detectable. DNA gel shift experiments suggested that might controlled by...

Abstract Most currently used antibiotics originate from Streptomycetes and phosphate limitation is an important trigger of their biosynthesis. Understanding the molecular processes underpinning such regulation crucial importance to exploit great metabolic diversity these bacteria get a better understanding role molecules in physiology producing bacteria. To contribute this field, comparative proteomic analysis two closely related model strains, Streptomyces lividans coelicolor was carried...

In most Streptomyces species, antibiotic production is triggered in phosphate limitation and repressed proficiency. However, the model strain, coelicolor , escapes this general rule produces actinorhoddin (ACT), a polyketide antibiotic, even more abundantly proficiency than limitation. ACT was shown to bear “anti-oxidant” properties suggesting that its biosynthesis by oxidative stress. Interestingly, lividans strain closely related S. does not produce any condition whereas pptA/sco4144...

The polyphosphate kinase gene (ppk) from Streptomyces lividans, which encodes a 774-amino-acid protein (86.4 kDa) showing extensive homology to other bacterial kinases, was cloned by polymerase chain reaction (PCR) using oligonucleotides derived the putative ppk closely related species, coelicolor. In vitro, purified Ppk shown be able synthesize [poly(P)] ATP (forward reaction) as well regenerate poly(P) in presence of an excess ADP (reverse reaction). conditions synthesis, phosphoenzyme...

Metabolic Flux Analysis is now viewed as essential to elucidate the metabolic pattern of cells and design appropriate genetic engineering strategies improve strain performance production processes. Here, we investigated carbon flux distribution in two Streptomyces coelicolor A3 (2) strains: wild type M145 its derivative mutant M1146, which gene clusters encoding four main antibiotic biosynthetic pathways were deleted. 13C-labeling allowed us reconstruct a map under steady-state conditions...

Recent physiological studies indicated that S. lividans metabolism was mainly glycolytic, whereas coelicolor oxidative. To determine whether such metabolic characteristics were correlated with consistent proteomics features, a comparative label-free, shotgun analysis of these strains carried out. Among 2024 proteins identified, 360 showed significant differences in abundance between the strains. This study revealed catabolized glucose less actively than lividans, amino acids present medium...

Antibiotics are often considered as weapons conferring a competitive advantage to their producers in ecological niche. However, since these molecules produced specific environmental conditions, notably phosphate limitation that triggers metabolic state, they likely play important roles the physiology of producing bacteria have been overlooked. Our recent experimental data well careful analysis scientific literature led us propose that, conditions moderate severe limitation-conditions known...

In the course of our research, aimed at improving sugar beets phosphorus nutrition, we isolated and characterized Streptomyces sp. strains, endemic from beet fields Beni-Mellal region, which are able to use natural rock phosphate (RP) tricalcium (TCP) as sole sources. Ten isolates yielded a comparable biomass in presence these two insoluble sources, indicating that they were extract similar amount (P) latter for their own growth. Interestingly, five strains released soluble P large excess...

ABSTRACT The overexpression of a regulatory gene the TetR family ( SCO3201 ) originating either from Streptomyces lividans or coelicolor was shown to strongly repress antibiotic production (calcium-dependent [CDA], undecylprodigiosin [RED], and actinorhodin [ACT]) S. ppk mutant strain . Curiously, this also had strong inhibitory effect on sporulation process but not that negatively regulate its own transcription, DNA binding motif found overlap −35 promoter sequence. interruption in did lead...

Streptomyces lividans TK24 is a strain that naturally produces antibiotics at low levels, but dramatic overproduction of occurs upon interruption the ppk gene. However, role Ppk enzyme in relation to regulation antibiotic biosynthesis remains poorly understood. In order gain better understanding phenotype mutant, proteomes wild-type (wt) and mutant strains, grown for 96 h on R2YE medium limited phosphate, were analyzed. Intracellular proteins separated two-dimensional (2D) gels, spots...

A regulatory gene, reg1, was identified in Streptomyces lividans. It encodes a 345-amino-acid protein (Reg1) which contains helix-turn-helix DNA-binding motif the N-terminal region. Reg1 exhibits similarity with LacI/GalR family members over entire sequence. displays 95% identity MalR (the repressor of malE S. coelicolor), 65% ORF-Sl (a putative gene alpha-amylase limosus), and 31% CcpA carbon catabolite Bacillus subtilis). In lividans, chromosomal disruption reg1 affected expression several...

Streptomycetes are well-known antibiotic producers possessing in their genomes numerous silent biosynthetic pathways that might direct the biosynthesis of novel bio-active specialized metabolites. It is thus great interest to find ways enhance expression these discover most needed antibiotics. In this study, we demonstrated over-expression acetyltransferase SCO0988 up-regulated production metabolites and accelerated sporulation weak producer, Streptomyces lividans deletion gene had opposite...

Abstract Chassis strains, derived from Streptomyces coelicolor M145, deleted for one or more of its four main specialized metabolites biosynthetic pathways (CPK, CDA, RED and ACT), in various combinations, were constructed the heterologous expression types origins. To determine consequences these deletions on metabolism strains comparative lipidomic metabolomic analyses original strain carried out. These studies unexpectedly revealed that deletion peptidic clusters, and/or a ACT cluster,...