A5021606008- Plant Stress Responses and Tolerance
- Fermentation and Sensory Analysis
- Hops Chemistry and Applications
- Plant Micronutrient Interactions and Effects
- Horticultural and Viticultural Research
- Postharvest Quality and Shelf Life Management
- Photosynthetic Processes and Mechanisms
- Vitamin C and Antioxidants Research
- Plant nutrient uptake and metabolism
- Plant Physiology and Cultivation Studies
- Chromatography in Natural Products
- Plant responses to elevated CO2
- Traumatic Brain Injury Research
- Genetic Mapping and Diversity in Plants and Animals
- Nutritional Studies and Diet
- Plant tissue culture and regeneration
- Liver Disease Diagnosis and Treatment
- Energy and Environment Impacts
- Antioxidant Activity and Oxidative Stress
- Air Quality and Health Impacts
- Plant biochemistry and biosynthesis
- Growth and nutrition in plants
- Plant Molecular Biology Research
- Banana Cultivation and Research
- Cancer Risks and Factors
University of Oxford
2015-2025
Genetics and Improvement of Fruit and Vegetables
2014-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2013-2024
Medical Research Council
2023
California Institute of Technology
2023
University of Strathclyde
2023
Boston Children's Hospital
2021-2022
Boston Children's Museum
2021
St Mary's University Twickenham London
2020
Ecological Society of America
2018
Abstract Genome-wide association studies have been successful in identifying genes involved polygenic traits and are valuable for crop improvement. Tomato (Solanum lycopersicum) is a major highly appreciated worldwide its health value. We used core collection of 163 tomato accessions composed S. lycopersicum, lycopersicum var cerasiforme, Solanum pimpinellifolium to map loci controlling variation fruit metabolites. Fruits were phenotyped broad range metabolites, including amino acids,...
Tobacco smoking is estimated to account for more than 1 million annual deaths in China, and the epidemic continues increase men. Large nationwide prospective studies linked different health records can help periodically assess disease burden attributed smoking. We aimed examine associations of with incidence mortality from an extensive range diseases China.We analysed data China Kadoorie Biobank, which recruited 512 726 adults aged 30-79 years, whom 210 201 were men 302 525 women....
A biologically based two-stage model for carcinogenesis is presented that relates events occurring at the cellular level to epidemiologic features of breast cancer in females. This model, which accommodates physiologic responses tissue menarche, menopause, and pregnancy, predicts age-specific incidence curves are close quantitative agreement with those observed six test populations: Connecticut, Denmark, Finland, Slovenia, Iceland, Osaka, Japan. According hormones influence epidemiology...
Quantitative trait loci (QTL) mapping is a step towards the identification of factors regulating traits such as fruit ascorbic acid content. A previously identified QTL controlling variations in tomato has been fine mapped and reveals that polygenic epistatic architecture. monodehydroascorbate reductase (MDHAR) allele candidate for proportion increase The MDHAR enzyme active different stages ripening, shows increased activity introgression lines containing wild-type (Solanum pennellii)...
Summary The GDP‐ d ‐mannose 3,5‐epimerase (GME, EC 5.1.3.18), which converts to l ‐galactose, is generally considered be a central enzyme of the major ascorbate biosynthesis pathway in higher plants, but experimental evidence for its role planta lacking. Using transgenic tomato lines that were RNAi‐silenced GME, we confirmed GME does indeed play key regulation plants. In addition, exhibited growth defects affecting both cell division and expansion. A further remarkable feature plants was...
Fresh fruit and vegetables are a major source of ascorbic acid (vitamin C), an important antioxidant for the human diet also plants. Ascorbic content in exhibits quantitative inheritance. Quantitative trait loci (QTL) have been mapped three tomato populations derived from crosses between cultivated varieties (Solanum lycopersicum accessions) related wild species or subspecies. The first population consists set introgression lines Solanum pennellii, each containing unique fragment genome....
Ascorbic acid is considered to be one of the most important antioxidants in plants and plays a vital role adaptation unfavorable conditions. In present study, an ascorbate oxidase gene (Solyc04g054690) was over-expressed cherry tomato cv. West Virginia 106 lines compared with previously studied RNAi silenced lines. Two lower activity (AO−15 AO−42), two elevated (AO+14 AO+16), non-transgenic line (WVa106) were grown irrigated 75 mM 150 NaCl 2015 2016. Growth, yield, chemical composition under...
ABSTRACT Background and Objective Previous observational studies reported a complex relationship between snoring coronary artery disease (CAD). We aimed to estimate the causal associations CAD among East Asian people, effects independent of BMI. Methods Based on 497,250 adults from China Kadoorie Biobank (CKB), we performed conventional prospective analysis outcomes, using multivariable Cox regression. also leveraged genome‐wide association (GWAS) summary statistics BMI CKB ( n = 100,626,...
The E2F transcription factors are key components of the cyclin D/retinoblastoma/E2F pathway. Here we demonstrate thatArabidopsis thaliana contains six functional AtE2F genes that all expressed in cell suspension culture but show different patterns expression during cycle progression. According to their structural and features, AtE2Fs can be divided into two distinct groups; although three members first group, AtE2Fa, AtE2Fb AtE2Fc, possess conserved domains found other plant animal E2Fs,...
We present a technique for easy, rapid analysis of both total and reduced forms vitamin C in fruits using microplates plate reader. This has been compared with spectrofluorometric classically used assaying fresh tomato. have applied these methods to population 118 tomato mutant lines controls search variability this trait. Six lines, identified as having high levels, four low levels chosen further study. The sugar concentration, dry matter content, fruit weight, titratable acidity, firmness....
Background and AimsThe mechanisms involving light control of vitamin C content in fruits are not yet fully understood. The present study aimed to evaluate the impact fruit leaf shading on ascorbate (AsA) accumulation tomato determine how sugar (as an AsA precursor) affected content.
The regulation of carbon allocation between photosynthetic source leaves and sink tissues in response to stress is an important factor controlling plant yield. Ascorbate oxidase apoplastic enzyme, which controls the redox state ascorbate pool. RNA interference was used decrease activity tomato (Solanum lycopersicum L.). Fruit yield increased these lines under three conditions where assimilate became limiting for wild-type plants: when fruit trusses were left unpruned, removed or water supply...
Flagellar type III secretion systems (T3SS) contain an essential cytoplasmic-ring (C-ring) largely composed of two proteins FliM and FliN, whereas analogous substructure for the closely related non-flagellar (NF) T3SS has not been observed in situ. We show that spa33 gene encoding putative NF-T3SS C-ring component Shigella flexneri is alternatively translated to produce both full-length (Spa33-FL) a short variant (Spa33-C), with required secretion. They associate 1:2 complex (Spa33-FL/C2)...
Summary Ascorbate content in plants is controlled by its synthesis from carbohydrates, recycling of the oxidized forms and degradation. Of these pathways, ascorbate degradation least studied represents a lack knowledge that could impair improvement fruits vegetables as non‐reversible leads to depletion pool. The present study revealed nature products using [ 14 C]ascorbate labelling tomato, model plant for fleshy fruits; oxalate threonate are accumulated leaves, oxalyl threonate....
The use of saline water for plant production will become increasingly necessary over future decades. In some cases, fruit quality such as in tomato, can be improved by irrigation with water. influence different salt concentrations on physiological responses and the expression selected genes cherry tomato (Solanum lycopersicum L), cv. West Virginia 106, was examined. Tomato plants were grown peatmoss substrate irrigated 0, 25, 50, 75, 100 or 150 mM sodium chloride (NaCl) a glasshouse. NaCl...
Ascorbate is a powerful antioxidant in plants, and its levels are an important quality criteria commercial species. Factors influencing these include environmental variations, particularly light, the genetic control of biosynthesis, recycling degradation. One genes involved pathway encodes monodehydroascorbate reductase (MDHAR), enzyme catalysing reduction oxidized radical ascorbate, monodehydroascorbate, to ascorbate. In MDHAR belongs multigene family. Here, we report presence isoform both...
GDP-D-mannose epimerase (GME, EC 5.1.3.18) converts to GDP-L-galactose, and is considered be a central enzyme connecting the major ascorbate biosynthesis pathway primary cell wall metabolism in higher plants. Our previous work demonstrated that GME crucial for both tomato. The aim of present study was investigate respective role two SlGME genes tomato by targeting each them through an RNAi-silencing approach. Taken individually SlGME1 SlGME2 allowed normal accumulation leaf fruits, thus...
Understanding how the fruit microclimate affects ascorbate (AsA) biosynthesis, oxidation and recycling is a great challenge in improving nutritional quality. For this purpose, tomatoes at breaker stage were harvested placed controlled environment conditions different temperatures (12, 17, 23, 27 31°C) irradiance regimes (darkness or 150 µmol m-2 s-1). Fruit pericarp tissue was used to assay ascorbate, glutathione, enzymes related oxidative stress AsA/glutathione cycle follow expression of...