A5080346196- Cancer Genomics and Diagnostics
- DNA Repair Mechanisms
- Cancer Treatment and Pharmacology
- Lung Cancer Treatments and Mutations
- Cancer Cells and Metastasis
- Bone health and treatments
- Oral and Maxillofacial Pathology
- Pancreatic and Hepatic Oncology Research
- Parathyroid Disorders and Treatments
- Protease and Inhibitor Mechanisms
- Cancer, Hypoxia, and Metabolism
- Single-cell and spatial transcriptomics
- 3D Printing in Biomedical Research
- CRISPR and Genetic Engineering
- Endometriosis Research and Treatment
- Cell Adhesion Molecules Research
- Endometrial and Cervical Cancer Treatments
- Parasite Biology and Host Interactions
- Reproductive System and Pregnancy
- CAR-T cell therapy research
- Hemodynamic Monitoring and Therapy
- Study of Mite Species
- Healthcare and Venom Research
- Cardiovascular Syncope and Autonomic Disorders
- Bone Metabolism and Diseases
Azienda Ospedaliera Pugliese Ciaccio
2017-2024
University of Basel
2016-2022
University Hospital of Basel
2018-2021
Utrecht University
2018
University Medical Center Utrecht
2018
Department of Health
1999
Circulating tumor cells (CTCs) are shed from solid cancers in the form of single or clustered cells, and latter display an extraordinary ability to initiate metastasis. Yet, biological phenomena that trigger shedding CTC clusters a primary cancerous lesion poorly understood. Here, when dynamically labeling breast cancer along progression, we observe majority undergoing hypoxia, while CTCs largely normoxic. Strikingly, find vascular endothelial growth factor (VEGF) targeting leads shrinkage,...
Molecular programs initiating cell fate divergence (CFD) are difficult to identify. Current approaches usually compare cells long after CFD initiation, therefore missing molecular changes at its start. Ideally, single that differ in their program but otherwise identical compared early CFD. This is possible diverging sister cells, which were until mother's division and thus mainly properties. In asymmetrically dividing divergent daughter fates prospectively committed during division, sisters...
The presence of circulating tumor cells (CTCs) in patients with breast cancer correlates to a bad prognosis. Yet, CTCs are detectable only minority progressive cancer, and factors that influence the abundance remain elusive. We conducted CTC isolation enumeration selected group 73 consecutive characterized by invasive high load treatment discontinuation at time isolation. were quantified Parsortix microfluidic device. Clinicopathological variables, blood counts detailed history prior...
Abstract Blood-borne metastasis of breast cancer involves a series tightly regulated sequential steps, including the growth primary tumor lesion, intravasation circulating cells (CTC), and adaptation in various distant metastatic sites. The genes orchestrating each these steps are poorly understood physiologically relevant contexts, owing to rarity experimental models that faithfully recapitulate biology, kinetics, tropism human cancer. Here, we conducted an vivo loss-of-function CRISPR...
The tumour microenvironment is a critical regulator of malignant cancer progression. While endothelial cells have been widely studied in the context angiogenesis, their role as modulators cell invasion and migration poorly understood. We investigated influence on invasive migratory behaviour human vitro. Upon exposure to culture supernatants cells, distinct such SK-BR-3 showed significantly increased concomitant with changes morphology gene expression reminiscent an epithelial-mesenchymal...
The nuclear receptor PPARγ is the master regulator of adipocyte differentiation, distribution, and function. In addition, induces terminal differentiation several epithelial cell lineages, including colon epithelia. Loss-of-function mutations in PPARG result familial partial lipodystrophy subtype 3 (FPDL3), a rare condition characterized by aberrant adipose tissue distribution severe metabolic complications, diabetes. Mutations have also been reported sporadic colorectal cancers, but...
Abstract Circulating tumor cells (CTCs) are derivatives of solid cancerous lesions that detach from the mass and enter blood circulation. CTCs considered to be precursors metastasis in several cancer types. They present patients as single or clusters, with latter being associated a higher metastatic potential. Methods eliminate bloodstream currently lacking. Here, we took advantage lower shear stress-resistance compared cells, developed device can without damage. The consists an axial pump...
Circulating tumor cells (CTCs) are precursors of metastasis in various cancer types. Many aspects regarding CTC biology remain poorly understood. Here, we describe mass spectrometric analysis CTCs from a breast xenograft mouse model, including procedures comprising enrichment, separation different subpopulations, and their quantitative proteomic assessment. This protocol aims to facilitate the identification protein content dynamics human that physiologically shed tumor-bearing xenografts,...
Summary An attempt to label the cercariae of Schistosoma japonicum with radioactive iron (Fe59) gave encouraging results. Adult flukes from that were exposed solutions total activities 2.4 µc, 0.9 µc and 0.1 respectively, for 6 hours definitely produced images in 30 42 days on photographic films by autoradiography. Similar results obtained another trial 2 iron.
Blood-borne metastasis accounts for most cancer-related deaths and involves circulating tumor cells (CTCs) that are successful in establishing new tumors at distant sites. CTCs found the bloodstream of patients as single (single CTCs) or multicellular aggregates (CTC clusters CTC-white blood cell clusters), with latter displaying a higher metastatic ability. Beyond enumeration, phenotypic molecular analysis is extraordinarily important to dissect CTC biology identify actionable...
Objective: The resistance index (RI) and the blood flow volume (Q a ) are most used Doppler ultrasound (DUS) parameters to identify presence of stenosis in arteriovenous fistula (AVF). However, reliability these indexes is now matter concern, particularly predicting subsequent thrombosis. In this study, we aimed at testing diagnostic capacity Q /RI ratio x for early identification AVF thrombosis risk stratification. Methods: From multicentre source population 336 HD patients, identified 119...
Abstract Endometriosis, affecting 1 in 9 women, presents treatment and diagnostic challenges. To address these issues, we generated the biggest single-cell atlas of endometrial tissue to date, comprising 466,371 cells from 35 endometriosis 25 non-endometriosis patients without exogenous hormonal treatment. Detailed analysis reveals significant gene expression changes altered receptor-ligand interactions present endometrium patients, including increased inflammation, adhesion, proliferation,...
The arteriovenous fistula constitutes the vascular access of first choice in hemodialysis. We present three clinical cases that highlight resolution interventional radiology venous stenosis, one major complications. Clinical monitoring and instrumental diagnostics with color Doppler ultrasound have prevented failure AVF due to high risk thrombosis. angiographic interventions, thanks collaboration between Spoke Hub, were completed without
This study aimed to identify unique characteristics in the peripheral blood mononuclear cells (PBMCs) of endometriosis patients and develop a non-invasive early diagnostic tool. Using single-cell RNA sequencing (scRNA-seq), we constructed first atlas PBMCs from based on 107,964 25,847 genes. Within CD16
Blood-borne metastasis accounts for most cancer-related deaths and involves circulating tumor cells (CTCs) that are successful in establishing new tumors at distant sites. CTCs found the bloodstream of patients as single (single CTCs) or multicellular aggregates (CTC clusters CTC-white blood cell clusters), with latter displaying a higher metastatic ability. Beyond enumeration, phenotypic molecular analysis is extraordinarily important to dissect CTC biology identify actionable...
<div>Abstract<p>Blood-borne metastasis of breast cancer involves a series tightly regulated sequential steps, including the growth primary tumor lesion, intravasation circulating cells (CTC), and adaptation in various distant metastatic sites. The genes orchestrating each these steps are poorly understood physiologically relevant contexts, owing to rarity experimental models that faithfully recapitulate biology, kinetics, tropism human cancer. Here, we conducted an <i>in...
Supplementary Figure from An <i>In Vivo</i> CRISPR Screen Identifies Stepwise Genetic Dependencies of Metastatic Progression