A5038850576- Cancer Genomics and Diagnostics
- DNA Repair Mechanisms
- Lung Cancer Treatments and Mutations
- Prostate Cancer Treatment and Research
- Cancer Treatment and Pharmacology
- Bladder and Urothelial Cancer Treatments
- Prostate Cancer Diagnosis and Treatment
- Cancer Cells and Metastasis
- Urinary and Genital Oncology Studies
- Breast Lesions and Carcinomas
- Urologic and reproductive health conditions
- Single-cell and spatial transcriptomics
- Cancer Immunotherapy and Biomarkers
- Cancer survivorship and care
- CAR-T cell therapy research
- Salivary Gland Tumors Diagnosis and Treatment
- Breast Cancer Treatment Studies
- Epigenetics and DNA Methylation
- Cancer and Skin Lesions
- Occupational and environmental lung diseases
- Immunotherapy and Immune Responses
- Esophageal Cancer Research and Treatment
- Cancer Research and Treatments
- Radiomics and Machine Learning in Medical Imaging
- Advanced Biosensing Techniques and Applications
University Hospital of Basel
2015-2024
Hospital Base
2019-2024
University of Basel
2014-2024
GastroZentrum Hirslanden Zürich
2023
St. Claraspital
2023
Kantonsspital Graubünden
2022-2023
University of Zurich
2023
Hôpital Orthopédique de la Suisse Romande
2023
St. James's Hospital
2020
Taipei Institute of Pathology
2019
Increasing evidence suggests that antibody-drug conjugates (ADCs) can enhance anti-tumor immunity and improve clinical outcome. Here, we elucidate the therapeutic efficacy immune-mediated mechanisms of a novel HER2-targeting ADC bearing potent anthracycline derivate as payload (T-PNU) in human HER2-expressing syngeneic breast cancer model resistant to trastuzumab ado-trastuzumab emtansine. Mechanistically, component induced immunogenic cell death leading exposure secretion danger-associated...
Patient-derived organoids (PDOs) represent promising preclinical models in various tumor types. In the context of prostate cancer (PCa), however, their establishment has been hampered by poor success rates, which impedes broad use for translational research applications. Along with necessity to improve culture conditions, there is a need identify factors influencing outcomes and determine how assess versus failure organoid generation. present study, we report our unbiased efforts generate...
The molecular repertoire promoting cancer cell plasticity is not fully elucidated. Here, we propose that glycosphingolipids (GSLs), specifically the globo and ganglio series, correlate promote transition between epithelial mesenchymal cells. character of ovarian remains stable throughout disease progression, spatial glycosphingolipidomics reveals elevated globosides in tumor compartment compared with ganglioside-rich stroma. CRISPR-Cas9 knockin mediated truncation endogenous E-cadherin...
The extracellular matrix (ECM) plays critical roles in tumor progression and metastasis. However, the contribution of ECM proteins to early metastatic onset peritoneal cavity remains unexplored. Here, we suggest a new route metastasis through interaction integrin alpha 2 (ITGA2) with collagens enriched coinciding poor outcome patients ovarian cancer. Using multiple gene-edited cell lines patient-derived samples, demonstrate that ITGA2 triggers cancer adhesion collagen, promotes migration,...
Abstract Background There is an urgent need for preclinical models of prostate cancer; however, clinically relevant patient‐derived cancer xenografts (PDXs) are demanding to establish. Methods Sixty‐seven patients who were undergoing palliative transurethral surgery or radical prostatectomy histologically confirmed, included in the study. Fresh tissue was identified by frozen analysis 48 patients. The transplanted subcutaneously and under renal capsule NSG NOG mice supplemented with human...
BK polyomavirus has been linked to urothelial carcinoma in immunosuppressed patients. Here, we performed comprehensive genomic analysis of a polyomavirus-associated, metachronous, multifocal and metastatic micropapillary cancer kidney transplant recipient. Dissecting heterogeneity by sorting technologies prior array-comparative hybridization followed short tandem repeat revealed that the was donor origin (4-year-old male). The top 50 cancer-associated genes showed no key driver mutations as...
The mechanisms by which prostate cancer shifts from an indolent castration-sensitive phenotype to lethal castration-resistant (CRPC) are poorly understood. Identification of clinically relevant genetic alterations leading CRPC may reveal potential vulnerabilities for therapy. Here we find that CUB domain-containing protein 1 (CDCP1), a transmembrane acts as substrate SRC family kinases (SFKs), is overexpressed in subset CRPC. Notably, CDCP1 cooperates with the loss tumor suppressor gene PTEN...
Abstract Sarcomatoid Urothelial Bladder Cancer (SARC) is a rare and aggressive histological subtype of bladder cancer for which therapeutic options are limited experimental models lacking. Here, we report the establishment long-term 3D organoid-like model derived from SARC patient ( SarBC-01 ). emulates morphological, phenotypical, transcriptional features harbors somatic mutations in genes frequently altered sarcomatoid tumors such as TP53 (p53) RB1 (pRB). High-throughput drug screening,...
BACKGROUND Rearrangements of the ROS1 oncogene are found in 1% to 2% non‐small cell lung cancers (NSCLC) and regarded as mutually exclusive oncogenic driver mutations. Since approval targeted therapy for ROS1‐positive NSCLC, testing has become a part diagnostic routine. Fluorescence situ hybridization (FISH), optionally selected by immunohistochemistry on histological material, is common practice detection rearrangements. However, NSCLC often diagnosed cytology alone, requiring predictive...
The Paris System (TPS) was introduced in the diagnostic routine with goal to simplify and standardise reporting of urinary cytology. categories TPS are based on defined cytological criteria, a focus high-grade urothelial carcinoma (HGUC). While 'negative for HGUC (NHGUC)' 'HGUC' straightforward, 'atypical cells (AUC)' 'suspicious (SHGUC)' remain inconclusive. In this study, we evaluated feasibility daily practice special emphasis ancillary fluorescence situ hybridisation (FISH) testing...
Background: Liquid biopsies offer significant potential for informing on cancer progression and therapeutic resistance via minimally invasive serial monitoring of genetic alterations. Although the epigenome is a central driving force in most neoplasia, accuracy tumor methylome using liquid remains relatively unknown. Objectives: to investigate how well two types biopsy (urine blood) capture prostate methylome, may thus serve as non-invasive surrogate studying epigenome. Methods: A cohort...
Abstract Blood-borne metastasis of breast cancer involves a series tightly regulated sequential steps, including the growth primary tumor lesion, intravasation circulating cells (CTC), and adaptation in various distant metastatic sites. The genes orchestrating each these steps are poorly understood physiologically relevant contexts, owing to rarity experimental models that faithfully recapitulate biology, kinetics, tropism human cancer. Here, we conducted an vivo loss-of-function CRISPR...
Despite several treatment options and an initial high response rate to androgen deprivation therapy, the majority of prostate cancers will eventually become castration-resistant in metastatic stage (mCRPC). Androgen receptor splice variant 7 (ARV7) is one best-characterized (AR) variants whose expression circulating tumor cells (CTCs) has been associated with enzalutamide resistance. ARV7 analysis before during could identify patients requiring alternative systemic therapies. However, a...
Background Diagnosis of hepatoblastoma (HBL) is based on characteristic clinical and radiological presentation, young age marked elevation serum α‐fetoprotein (aFP). Fine needle aspiration (FNA) technique successfully used in the diagnosis hepatoblastoma. To evaluate value FNA subtyping HBL, we report our experience correlated to histological sections (core biopsy, CNB). Methods From 1991 2015, 21 cases from 20 patients were cytologically diagnosed as HBL. The 15 males 5 females, mean being...
Background Circulating tumour cells (CTCs) represent a morphologically distinct subset of cancer cells, which aid the metastatic spread. The ExPeCT trial aimed to examine effectiveness structured exercise programme in modulating levels CTCs and platelet cloaking patients with prostate cancer. Methods Participants (n = 61) were randomised into either standard care (control) or arms. Whole blood was collected for all participants at baseline (T0), three months (T3) six (T6), analysed presence...
Interactions between circulating tumour cells (CTCs) and platelets are thought to inhibit natural killer(NK)-cell-induced lysis. We attempted correlate CTC numbers in men with advanced prostate cancer platelet counts lymphocyte numbers. Sixty-one ExPeCT trial participants, divided into overweight/obese normal weight groups on the basis of a BMI ≥ 25 or <25, were randomized participate not six-month exercise programme. Blood samples at randomization, three six months, subjected ScreenCell...
MRI-targeted prostate biopsy improves detection of clinically significant cancer (PCa). However, up to 70% PCa lesions display intralesional tumor heterogeneity. Current target sampling strategies do not yet adequately account for this finding. This prospective study included 118 patients who underwent transperineal robotic assisted the prostate. We identified a total 58 PCa-positive PI-RADS lesions. compared diagnostic accuracy target-saturation strategy single, two, or three randomly...