A5050821617- Occupational and environmental lung diseases
- Sirtuins and Resveratrol in Medicine
- Advanced Glycation End Products research
- Autophagy in Disease and Therapy
- Cancer, Hypoxia, and Metabolism
- Adipose Tissue and Metabolism
- Immune cells in cancer
- MicroRNA in disease regulation
- S100 Proteins and Annexins
- HER2/EGFR in Cancer Research
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Cancer-related molecular mechanisms research
- Cancer, Stress, Anesthesia, and Immune Response
- Biomarkers in Disease Mechanisms
- Tissue Engineering and Regenerative Medicine
- Cell death mechanisms and regulation
- Cardiac Fibrosis and Remodeling
- Adenosine and Purinergic Signaling
- Telomeres, Telomerase, and Senescence
- Medical Imaging and Pathology Studies
- Mitochondrial Function and Pathology
- Advanced Breast Cancer Therapies
- Patient Dignity and Privacy
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Breast Cancer Treatment Studies
Sapienza University of Rome
2009-2025
Institute of Oncology Research
2019-2024
University of Hawaii System
2013-2023
University of Hawaii Cancer Center
2013-2023
University of Hawaiʻi at Mānoa
2013-2023
Cancer Center of Hawaii
2013-2023
Università della Svizzera italiana
2019-2020
University of Lausanne
2019
University of Groningen
2019
University Medical Center Groningen
2019
In liver the mitochondrial sirtuin, SIRT5, controls ammonia detoxification by regulating CPS1, first enzyme of urea cycle. However, while SIRT5 is ubiquitously expressed, cycle and CPS1 are only present in and, to a minor extent, kidney. To address possibility that involved production also nonliver cells, clones human breast cancer cell lines MDA-MB-231 mouse myoblast C2C12, overexpressing or silenced for were produced. Our results show increased SIRT5-silenced decreased SIRT5-overexpressing...
Accumulating senescent cells within tissues contribute to the progression of aging and age-related diseases. Botanical extracts, rich in phytoconstituents, present a useful resource for discovering therapies that could target senescence thus improve healthspan. Here, we show daily oral administration standardized extract Salvia haenkei (Haenkenium (HK)) extended lifespan healthspan naturally aged mice. HK treatment inhibited age-induced inflammation, fibrosis markers across several tissues,...
The role of hypoxia in regulating tumor progression is still controversial. Here, we demonstrate that, similarly to what previously observed by us human prostate and breast samples, increases expression the receptor for advanced glycation end products (RAGE) purinergic P2X7 (P2X7R). was shown fact that hypoxia-inducible factor (HIF)-1α silencing downregulated RAGE P2X7R protein levels as well nuclear factor-kappaB (NF-κB) expression. In contrast, NF-κB reduced without affecting or...
Adaptation to hypoxia and consequent pro-inflammatory gene expression of prostate breast carcinomas have been implicated in the progression toward cancer malignant phenotype. Only partial data are available for human tumor glioblastoma multiforme (GBM). The aim our study was analyze hypoxic microenvironment GBMs demonstrate that a stem/progenitor cell line derived from (GBM-SCs), activates coordinated inflammatory response, evidencing an invasive migratory phenotype.From each 10 solid...
Carriers of heterozygous germline BAP1 mutations (BAP1+/-) develop cancer. We studied plasma from 16 BAP1+/- individuals 2 families carrying different and 30 wild-type (BAP1WT) controls these same families. Plasma samples were analyzed by liquid chromatography time-of-flight mass spectrometry (LC-TOF-MS), ultra-performance triple quadrupole (UPLC-TQ-MS), gas (GC-TOF-MS). found a clear separation in the metabolic profile between BAP1WT individuals. confirmed specificity data vitro using 12...
Abstract Purpose: To determine whether serum levels of high mobility group box protein 1 (HMGB1) could differentiate malignant mesothelioma patients, asbestos-exposed individuals, and unexposed controls. Experimental Design: Hyperacetylated nonacetylated HMGB1 (together referred to as total HMGB1) were blindly measured in blood collected from patients (n = 22), individuals with verified chronic asbestos exposure 20), benign pleural effusions 13) or not due 25), healthy controls 20). Blood...
HIF1α and NFkB are two transcription factors very frequently activated in tumors involved tumor growth, progression, resistance to chemotherapy. In fact, together regulate of over a thousand genes that, turn, control vital cellular processes such as adaptation the hypoxia, metabolic reprograming, inflammatory reparative response, extracellular matrix digestion, migration invasion, adhesion, etc. Because this wide involvement they could an integrated manner origin malignant phenotype....
Abstract High-mobility group box 1 (HMGB1) is an inflammatory molecule that has a critical role in the initiation and progression of malignant mesothelioma (MM). Aspirin (acetylsalicylic acid, ASA) most widely used nonsteroidal anti-inflammatory drug reduces incidence, metastatic potential mortality many inflammation-induced cancers. We hypothesized ASA may exert anticancer properties MM by abrogating carcinogenic effects HMGB1. Using HMGB1-secreting -non-secreting human cell lines, we...
BAP1 is a powerful tumor suppressor gene characterized by haplo insufficiency. Individuals carrying germline mutations often develop mesothelioma, an aggressive malignancy of the serosal layers covering lungs, pericardium, and abdominal cavity. Intriguingly, mesotheliomas developing in carriers are less aggressive, these patients have significantly improved survival. We investigated apparent paradox that, when mutated, causes mesotheliomas. discovered that mesothelioma biopsies with...
Human malignant mesothelioma (MM) is an aggressive cancer linked to asbestos and erionite exposure. We previously reported that High-Mobility Group Box-1 protein (HMGB1), a prototypic damage-associated molecular pattern, drives MM development sustains progression. Moreover, we demonstrated targeting HMGB1 inhibited cell growth motility in vitro, reduced tumor vivo, prolonged survival of MM-bearing mice. Ethyl pyruvate (EP), the ethyl ester pyruvic acid, has been shown be effective inhibitor...
The role of tumor cells in synthesizing pro‐inflammatory molecules is still controversial. Here we report that hypoxic treatment the MCF‐7 human mammary adenocarcinoma cell line induced activation hypoxia‐inducible factor 1α (HIF‐1α) and nuclear factor‐kappa B (NF‐κB). Importantly, hypoxia regulated expression alarmin receptors such as receptor for advanced glycation end products (RAGE) purinoreceptor (P2X7R), up‐regulated inflammatory response (IR) genes inducible enzymes nitric oxide...
The following study demonstrated that, in vitro differentiated neurons, SIRT1 silencing induced an increase of IGF-1 protein expression and secretion IGF-1R levels which, turn, prolonged neuronal cell survival presence apoptotic insult. On the contrary, overexpression increased death. In particular, were negatively regulated by SIRT1. silenced cells, was associated to AKT ERK1/2 phosphorylation. Moreover, differentiation reduced overexpressing cells cells. We conclude that neurons appear...
Sirtuins are conserved NAD+ -dependent deacylases. SIRT1 is a nuclear and cytoplasmic sirtuin involved in the control of histones transcription factors function. SIRT3 mitochondrial protein, which regulates Although, both have been implicated resistance to cellular stress, link between these two sirtuins has not studied so far. Here we aimed unravel: i) role SIRT1-SIRT3 axis for response oxidative stress DNA damage; ii) how mammalian cells modulate such mechanisms involved. Therefore,...
The mechanisms by which prostate cancer shifts from an indolent castration-sensitive phenotype to lethal castration-resistant (CRPC) are poorly understood. Identification of clinically relevant genetic alterations leading CRPC may reveal potential vulnerabilities for therapy. Here we find that CUB domain-containing protein 1 (CDCP1), a transmembrane acts as substrate SRC family kinases (SFKs), is overexpressed in subset CRPC. Notably, CDCP1 cooperates with the loss tumor suppressor gene PTEN...
Similar to asbestos fibers, nonregulated mineral fibers can cause malignant mesothelioma (MM). Recently, increased proportions of women and young individuals with MM were identified in southern Nevada, suggesting that environmental exposure carcinogenic was causing the development MM. Palygorskite, a fibrous silicate history possible carcinogenicity, is abundant Nevada. In this study, our aim determine whether palygorskite contributing While palygorskite, vitro, displayed some cytotoxicity...