A5044201188- Pregnancy and preeclampsia studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Proteoglycans and glycosaminoglycans research
- SARS-CoV-2 and COVID-19 Research
- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Advanced Proteomics Techniques and Applications
- Gestational Diabetes Research and Management
- HER2/EGFR in Cancer Research
- Organ Transplantation Techniques and Outcomes
- Protein purification and stability
- Viral Infections and Immunology Research
- HIV Research and Treatment
- Viral Infectious Diseases and Gene Expression in Insects
- Renal and Vascular Pathologies
- Protease and Inhibitor Mechanisms
- Cancer-related molecular mechanisms research
- Chemical Synthesis and Analysis
- Animal Virus Infections Studies
- Transplantation: Methods and Outcomes
- Click Chemistry and Applications
- Carbohydrate Chemistry and Synthesis
- HIV/AIDS drug development and treatment
- Immune cells in cancer
- Peroxisome Proliferator-Activated Receptors
Icahn School of Medicine at Mount Sinai
2023-2025
Allen Institute for Brain Science
2023-2025
Kantonsspital Baselland
2024
Université de Strasbourg
2023
Medical University of South Carolina
2021-2023
Heart and Diabetes Center North Rhine-Westphalia
2007-2022
Ruhr University Bochum
2011-2022
Institute for Transfusion Medicine
2021
University of Basel
2020
Bielefeld University
2011-2019
Immunocompromised patients have been excluded from studies of SARS-CoV-2 messenger RNA vaccines. The immune response to vaccines against other infectious agents has shown be blunted in such patients. We aimed analyse the humoral and cellular prime-boost vaccination with BNT162b2 vaccine (Pfizer-BioNTech) cardiothoracic transplant recipients.A total 50 [1-3 years post heart (42), lung (7), or heart-lung (1) transplant, mean age 55 ± 10 years] a control group healthy staff members were...
Decorin and oxidized low-density lipoprotein (Ox-LDL) independently induce osteogenic differentiation of vascular smooth muscle cells (VSMCs). We aimed to determine whether decorin glycosaminoglycan (GAG) chain synthesis contributes Ox-LDL-induced calcification human VSMCs in vitro.Human treated with Ox-LDL oxidative stress showed increased alkaline phosphatase (ALP) activity, accelerated mineralization, a difference both GAG biosynthesis CS/DS structure compared untreated controls. mRNA...
Recently released sequence information on Chinese hamster ovary (CHO) cells promises to not only facilitate our understanding of these industrially important cell factories through direct analysis the sequence, but also enhance existing methodologies and allow new tools be developed. In this article we demonstrate utilization CHO specific improve mass spectrometry (MS) based proteomic identification. The use various databases enabled identification 282 additional proteins, thus increasing...
Multiple, site-specific protein conjugation is increasingly attractive for the generation of antibody–drug conjugates (ADCs). As it important to control number and position cargoes in an ADC, position-selective reactive sites interest required. Formylglycine (FGly) residues are generated by enzymatic conversion cysteine embedded a certain amino acid sequence motif with formylglycine-generating enzyme (FGE). The addition copper ions increases FGE activity leading cysteines within less readily...
Nucleic acid amplification techniques (NAT) in routine blood donor screening considerably reduce the diagnostic window phase period. Nevertheless, several reports of false-negative NAT results were published. Here, four cases human immunodeficiency virus Type 1 (HIV-1) RNA-positive donations that escaped detection by are described.A total 2.7 million screened for viral infections between January 2010 and October 2012 our German Red Cross donation service. Four plasma specimens with...
The G2019S mutation in the leucine-rich repeat kinase 2 (LRRK2) gene is a major risk factor for development of Parkinson's disease (PD). LRRK2, although ubiquitously expressed, highly abundant cells innate immune system. Given importance central and peripheral PD, we sought to investigate consequences on microglial monocyte transcriptome function. We have generated large-scale transcriptomic profiles isogenic human induced (iMGLs) patient derived monocytes carrying under baseline culture...
Background. Laparoscopic living kidney nephrectomy is thought to be associated with reduced morbidity, when compared open nephrectomy. The purpose of this study was explore the impact these techniques on donors' clinical outcomes, satisfaction and motivation donate.
The development of simple molecular assays with membrane protein receptors in a native conformation still represents challenging task. Exosomes are extracellular vesicles which, due to their stability and small size, suited for analysis various assay formats. Here, we describe novel approach sort recombinant fully functional proteins exosomes using targeting peptide. Specific binding high affinity ligands the potassium channel Kv1.2, G-protein coupled receptor CXCR4, botulinum neurotoxin...
Epidermal growth factor receptor (EGFR) is a validated tumor marker overexpressed in various cancers such as squamous cell carcinoma (SSC) of the head and neck gliomas. We constructed protein-drug conjugates based on anti-EGFR Designed Ankyrin Repeat Protein (DARPin) E01, compared bivalent DARPin dimer (DD1) DARPin-Fc (DFc) to monomeric (DM) antibody derived scFv425-Fc (scFvFc) culture mouse model. The modular conjugation system, which was successfully applied for preparation -dye...
Abstract Microglia, the immune cells of brain, are increasingly implicated in neurodegenerative disorders through genetic studies. However, how risk factors for these diseases related to microglial gene expression, function, and ultimately disease, is still largely unknown. Microglia change rapidly response alterations their cellular environment, which regulated changes transcriptional programs, as yet poorly understood. Here, we compared effects a set inflammatory restorative stimuli...
Human xylosyltransferase I catalyzes the initial and rate-limiting step in biosynthesis of glycosaminoglycans proteoglycans. Furthermore, this enzyme has been shown to play a major role physiological development bone cartilage as well pathophysiological processes such systemic sclerosis, dilated cardiomyopathy, or fibrosis. Here, we report for first time identification characterization XYLT1 gene promoter region important transcription factors involved its regulation. Members activator...
The molecular basis of TNF tolerance is poorly understood. In human monocytes we detected two forms refractoriness, as follows: absolute was selective, dose dependently affecting a small group powerful effector molecules; induction represented more general phenomenon. Preincubation with high induces both and tolerance, whereas low-dose preincubation predominantly mediates tolerance. cells preincubated the dose, observed blockade IκBα phosphorylation/proteolysis nuclear p65 translocation....
The alarmone species ppGpp and pppGpp are elementary components of bacterial physiology as they both coordinate the stress response serve fine-tuners general metabolism during conditions balanced growth. Since regulation (p)ppGpp effects on cellular processes highly complex show massive differences between species, underlying molecular mechanisms have so far only been insufficiently investigated for numerous microorganisms. In this study, in actinobacterial model organism Corynebacterium...
The epidermal growth factor receptor (EGFR) is a transmembrane protein involved in cell signaling processes, and dysregulation of its activity often drives tumor growth. EGFR clinically validated marker target for antibodies tyrosine kinase inhibitors. We demonstrate that fusion the natural ligand (EGF) with fluorescent reporter mCherry can be expressed cytosol E. coli high yields biological activity. Biophysical characterization by mass spectrometry analysis confirmed three disulfide bonds...
The novel coronavirus disease 2019 (COVID-19), caused by the severe acute respiratory syndrome 2 (SARS-CoV-2), has spread across world. aim of our study was to characterize mild courses and determine antibody status for these patients.
We report the results of a prospective study on immunogenicity 3rd dose BNT162b2 in thoracic organ recipients with no or minimal response following two-dose vaccination scheme.A total 243 transplant received homologue dose. Anti-SARS-CoV2-immunoglobulins (IgGs) were monitored immediately before (T1), 4 weeks (T2) as well 2 and months after Neutralizing antibody capacity (NAC) was determined at T2. To reveal predictors for detectable humoral response, patients divided into positive group (n =...
Abstract Microglia, the innate immune cells of central nervous system, have been genetically implicated in multiple neurodegenerative diseases. We previously mapped genetic regulation gene expression and mRNA splicing human microglia, identifying several loci where common variants microglia-specific regulatory elements explain disease risk identified by GWAS. However, effects on has challenging due to use short sequencing reads identify causal isoforms. Here we present isoform-centric...
Paradoxical deterioration in patients with tuberculous meningitis is a significant diagnostic and treatment challenge. We present the case of 55-year-old previously healthy male, human immunodeficiency virus (HIV)-negative, presenting headache, fever, agitation slurred speech, who was ultimately diagnosed meningoencephalitis confirmed by cerebrospinal fluid polymerase chain reaction positive for Mycobacterium tuberculosis complex. After initiation standard antitubercular therapy combined...