A5045794851- RNA Research and Splicing
- Advanced NMR Techniques and Applications
- NMR spectroscopy and applications
- RNA modifications and cancer
- RNA regulation and disease
- COVID-19 Impact on Reproduction
- Immune Cell Function and Interaction
- Metabolism and Genetic Disorders
- RNA and protein synthesis mechanisms
- Spectroscopy and Quantum Chemical Studies
- Amino Acid Enzymes and Metabolism
- Cytokine Signaling Pathways and Interactions
- Electron Spin Resonance Studies
- Soil and Water Nutrient Dynamics
- Proteins in Food Systems
- Plant Toxicity and Pharmacological Properties
- Immune cells in cancer
- Molecular spectroscopy and chirality
- Cancer Research and Treatments
- Cytomegalovirus and herpesvirus research
- Immune Response and Inflammation
- Enzyme Catalysis and Immobilization
- Microbial bioremediation and biosurfactants
- Microbial Metabolites in Food Biotechnology
- Phosphorus and nutrient management
University of Hohenheim
2019-2024
Klinik für Frauenheilkunde
2022-2024
Medizinische Hochschule Hannover
2013-2024
Technical University of Munich
2012-2021
Helmholtz Zentrum München
2012-2021
Center for Integrated Protein Science Munich
2017-2019
Schiller International University
1968
The strength of antiviral T cell responses correlates with clearance hepatitis B virus (HBV) infection, but the immunological mechanisms mitigating or suppressing HBV-specific cells are still poorly understood. In this study, we examined role CD4+ Foxp3+ regulatory (Tregs) in a mouse model acute HBV infection. We initiated infection via an adenoviral vector transferring 1.3-fold overlength genome (AdHBV) into transgenic DEREG mice, where Tregs can be transiently selectively depleted by...
NMR spectroscopy is a powerful technique to study ribonucleic acids (RNAs) which are key players in plethora of cellular processes. Although the toolbox for structural studies RNAs expanded during last decades, they often remain challenging. Here, we show that solvent paramagnetic relaxation enhancements (sPRE) induced by soluble, compound Gd(DTPA-BMA) provide quantitative measure RNA accessibility and encode distance-to-surface information correlates well with structure improves accuracy...
The enzymic flux rates through the Embden‐Meyerhof pathway and nucleotides of liver have been measured in vivo with [ 32 P]P i ‐tracer technique. specific radioactivities 15 positions 11 metabolites within first minute after injection carrier‐free . results treated analogue computer main conclusions are: turnover all compounds examined is range from seconds to minutes. velocity oxidative phosphorylation so high that 30 sec tracer prestationary kinetics observed, indicating γ‐ P position ATP...
In infected cells, Epstein-Barr virus (EBV) alternates between latency and lytic replication. The viral bZIP transcription factor ZEBRA (Zta, BZLF1) regulates this cycle by binding to two classes of response elements (ZREs): CpG-free motifs resembling the consensus AP-1 site recognized cellular proteins CpG-containing that are selectively bound upon cytosine methylation. We report structural mutational analysis a CpG-methylated ZRE (meZRE) from promoter. recognizes CpG methylation marks...
NMR spectroscopy at ultra-high magnetic fields requires improved radiofrequency (rf) pulses to cover the increased spectral bandwidth. Optimized 90° pulse pairs were introduced as Ramsey-type cooperative (Ram-COOP) for biomolecular applications. The Ram-COOP element provides broadband excitation with enhanced sensitivity and reduced artifacts even >1.0 GHz 1 H Larmor frequency (23 T). A pair of 30 μs achieves an bandwidth 100 kHz a maximum rf field 20 kHz, more than three-fold compared by...
Throughout metazoans, Staufen (Stau) proteins are core factors of mRNA localization particles. They consist three to four double-stranded RNA binding domains (dsRBDs) and a C-terminal dsRBD-like domain. Mouse Staufen2 (mStau2)-like Drosophila Stau (dmStau) contains dsRBDs. Existing data suggest that only dsRBDs 3-4 necessary sufficient for binding. Here, we show 1 2 mStau2 bind with similar affinities kinetics as 3 4. While by these tandem is transient, all recognize their target RNAs high...
Experimental procedures and conditions are described for the determination of specific radioactivities 16 different P ‐positions in 12 phosphorylated liver compounds after labelling vivo with [ 32 P]orthophosphate. These data necessary calculations flux rates within mononucleotides glycolytic pathway. Purification following given: ATP, ADP, GTP, UTP, UDPG, glucose 6‐phosphate, fructose 1,6‐biphosphate, dihydroxyacetone‐phosphate, glycerol 3‐phosphate, phosphoglyceric acid...
Furan fatty acids (FuFAs) are a class of naturally occurring minor with fish as the richest food source. Typically, FuFA analysis is cumbersome and involves several steps. We developed quantitative 1H NMR method (qNMR) in which oil samples were directly measured after dilution CDCl3 stabilized silver (which was essential to prevent formation radicals) addition an internal standard. The singlet at δ = 1.89 ppm suitable for quantitation monomethyl FuFAs, whereas signal 1.83 quantitate dimethyl...
The molecular basis of TNF tolerance is poorly understood. In human monocytes we detected two forms refractoriness, as follows: absolute was selective, dose dependently affecting a small group powerful effector molecules; induction represented more general phenomenon. Preincubation with high induces both and tolerance, whereas low-dose preincubation predominantly mediates tolerance. cells preincubated the dose, observed blockade IκBα phosphorylation/proteolysis nuclear p65 translocation....
The time-resolved impact of monensin on the active rumen microbiome was studied in a rumen-simulating technique (Rusitec) with metaproteomic and metabolomic approaches. Monensin treatment caused decreased fibre degradation potential that observed by reduced abundance proteins assigned to fibrolytic bacteria glycoside hydrolases, sugar transporters carbohydrate metabolism. Decreased proteolytic activities resulted amounts ammonium as well branched-chain fatty acids. family Prevotellaceae...
Abstract NMR spectroscopy at ultra‐high magnetic fields requires improved radiofrequency ( rf ) pulses to cover the increased spectral bandwidth. Optimized 90° pulse pairs were introduced as Ramsey‐type cooperative (Ram‐COOP) for biomolecular applications. The Ram‐COOP element provides broadband excitation with enhanced sensitivity and reduced artifacts even >1.0 GHz 1 H Larmor frequency (23 T). A pair of 30 μs achieves an bandwidth 100 kHz a maximum field 20 kHz, more than three‐fold...
Abstract Background and aims Soil organic phosphorus (P org ) is of interest for plant nutrition because it can comprise between 20 80% total soil phosphate (P). This study to examine the effect compost application on phosphatases microbial biomass, which influence P turnover and, furthermore, speciation . Methods from a long-term field experiment (since 1997) compares with inorganic fertilization was analyzed calcium-acetate-lactate extractable (CAL-P), Olsen-P, acid (Acid-P ase alkaline...
Objective: We aimed to investigate the difference in symptom prevalence and association between symptoms adverse pregnancy outcomes during dominance of Wild-type/Alpha, Delta, Omicron.
Kooperative (COOP) Pulse , die so enworfen wurden, dass sie gegenseitig ihre Imperfektionen kompensieren, können Leistungsfähigkeit von NMR-Experimenten signifikant erhöhen. S. Asami, J. Glaser, M. Sattler et al. demonstrieren in ihrer Zuschrift auf 14706 ff., Paare COOP-π/2-Pulsen typische biomolekulare NMR-Experimente stark verbessern, indem bei ultrahohen Magnetfeldern den gesamten interessierenden Spektralbereich ausleuchten. Hier visualisiert ist ein optimiertes Paar COOP-Pulsen durch...
Abstract Throughout metazoans, Staufen (Stau) proteins are core factors of mRNA localization particles. They consist three to four double-stranded RNA binding domains (dsRBDs) and a C-terminal dsRBD-like domain. Mouse Staufen2 (mStau2) like Drosophila Stau (dmStau) contains dsRBDs. Existing data suggest that only dsRBDs 3-4 necessary sufficient for binding. Here, we show 1 2 mStau2 bind with similar affinities kinetics as 3 4. While by these tandem is transient, all recognize their target...
Einleitung Beobachtungsstudien über schwangere Frauen, die mit COVID-19 infiziert sind, berichten von einem erhöhten Risiko für FGR. Dies ist erste Studie, Daten aus prospektiven SARS-Cov 2-Register in der Schwangerschaft verwendet und Entwicklung AGA-Feten zu FGR bei Geburt nach einer mütterlichen Covid-19-Infektion untersucht. Ziel dieser Studie war es, Hypothese überprüfen, dass Rate an COVID-19-Infektion erhöht ist.