A5009548645- Immune Cell Function and Interaction
- IL-33, ST2, and ILC Pathways
- T-cell and B-cell Immunology
- Diabetes Management and Research
- Immune cells in cancer
- Hematopoietic Stem Cell Transplantation
- Acute Lymphoblastic Leukemia research
- Endometriosis Research and Treatment
- Reproductive System and Pregnancy
- CAR-T cell therapy research
- Cancer Immunotherapy and Biomarkers
- Graphene and Nanomaterials Applications
- Nanoparticle-Based Drug Delivery
- Immunodeficiency and Autoimmune Disorders
- Peptidase Inhibition and Analysis
- Chronic Lymphocytic Leukemia Research
- 3D Printing in Biomedical Research
- Neuroscience and Neural Engineering
- Immunotherapy and Immune Responses
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Eosinophilic Esophagitis
- Monoclonal and Polyclonal Antibodies Research
- Advanced biosensing and bioanalysis techniques
- Botulinum Toxin and Related Neurological Disorders
- Biochemical and Molecular Research
Ospedale Policlinico San Martino
2014-2025
Istituto Giannina Gaslini
2014-2019
Istituti di Ricovero e Cura a Carattere Scientifico
2016-2017
Alleanza Contro il Cancro
2010
Laboratory of Molecular Genetics
2006-2007
Tertiary lymphoid structures (TLSs) are a common finding in non-small cell lung cancer (NSCLC) and predictors of favourable clinical outcome. Here we show that NCR+ innate (ILC)-3 present the infiltrate human NSCLC mainly localized at edge tumour-associated TLSs. This intra-tumoral lymphocyte subset is endowed with tissue-inducing properties and, on activation, produces IL-22, TNF-α, IL-8 IL-2, activates endothelial cells. Tumour NCR+ILC3 may interact both tumour cells fibroblasts, resulting...
Decidual and uterine Natural Killer (NK) cells have been shown to contribute the successful pregnancy both in humans mice. NK represent "cytotoxic" group 1 innate lymphoid (ILCs) are distinct from recently described "helper" ILC1. Here we show that mice majority of ILC endometrium/uterus decidua express Eomesodermin (Eomes), thus suggesting they developmentally related conventional cells. However, differed peripheral In Eomes+ decidual expressed CD49a other markers tissue residency including...
Thanks to their biocompatibility and high cargo capability, graphene-based materials (GRMs) might represent an ideal brain delivery system. The capability of GRMs reach the has mainly been investigated in vivo highlighted some controversy. Herein, we employed two vitro BBB models increasing complexity investigate bionano interactions with graphene oxide (GO) few-layer (FLG): a 2D murine Transwell model, followed by 3D human multicellular assembloid, mimic architecture intercellular...
Background Due to their enhanced responsiveness and persistence, cytokine-induced memory-like (CIML)-natural killer (NK) cells have emerged as new immunotherapeutic tools against malignancies. However, effects on tumor-cell spread metastases in solid tumors remain poorly investigated. Moreover, a clear identification of the most effective CIML-NK subsets, especially controlling cancer stem (CSC), is still lacking. Methods We performed combined phenotypical functional analyses cell either...
Natural killer (NK) cells play a key role in immunity, but how HLA class I (HLA-I) and cell immunoglobulin-like receptor 3DL1 (KIR3DL1) polymorphism impacts disease outcome remains unclear. KIR3DL1 (*001/*005/*015) tetramers were screened for reactivity against panel of HLA-I molecules. This revealed different distinct hierarchies specificity each allotype, with KIR3DL1*005 recognizing the widest array ligands. These differences further reflected functional studies using NK clones expressing...
Abstract Crosstalk mechanisms between pericytes, endothelial cells, and astrocytes preserve integrity function of the blood-brain-barrier (BBB) under physiological conditions. Long intercellular channels allowing transfer small molecules organelles distant cells called tunneling nanotubes (TNT) represent a potential substrate for energy matter exchanges tripartite cellular compartments BBB. However, role TNT across BBB conditions in course dysfunction is unknown. In this work, we analyzed...
Natural killer (NK) cells represent a promising cell type in antitumor immunotherapy for efficacy and safety, particularly the treatment of hematologic malignancies. NK have been shown to exert antileukemia activity context haploidentical hematopoietic stem transplantation (haplo-HSCT). Products developed boost activation only when cross-linked by tumor cells, avoiding any off-target effect. Here, we tested vitro effect different NK-cell engagers (NKCE), which trigger either NKp46 or NKp30...
Human NK cells activity against cancer is deeply suppressed by TGF-β1, an immunomodulatory cytokine that released and activated in the tumor microenvironment. Moreover, our previous data showed TGF-β1 modifies chemokine receptor repertoire of cells. In particular, it decreases expression CX3CR1 drives these effectors toward peripheral tissues, including sites. order to identify possible mechanisms mediating receptors modulation, we analyzed miRNA profile TGF-β1-treated primary The analysis...
Natural killer cells are cytotoxic innate lymphoid that play an important role for early host defenses against infectious pathogens and surveillance tumor. In humans, NK may be divided in various subsets on the basis of relative CD56 expression low-affinity FcγRIIIA CD16. particular, two main cell represented by CD56bright/CD16-/dull CD56dull/CD16bright cells. Experimental evidences indicate CD56bright CD56dull represent different maturative stages developmental pathway. We identified...
Abstract Recently, cancer research protocols have introduced clinical-stage spirooxindole-based MDM2 inhibitors. However, several studies reported tumor resistance to the treatment. This directed efforts invest in designing various combinatorial libraries of spirooxindoles. Herein, we introduce new series spirooxindoles via hybridization chemically stable core spiro[3 H -indole-3,2′-pyrrolidin]-2(1 )-one and pyrazole motif inspired by lead pyrazole-based p53 activators, inhibitor BI-0252...
The history of insulin-induced skin lipohypertrophy (LH) runs parallel to that insulin's 100 years, and an average 47% insulin-treated patients still suffer from it today. metabolic economic effects LH are significant, with hypoglycemia being the most striking. objective study was perform a 52-week follow-up 713 type 2 diabetes (T2DM) detect any differences in occurrence hypoglycemic events (HYPOs) related healthcare costs as well rates injection habits between intensive education...
Mesenchymal stromal cells (MSC) present in the tumor microenvironment [usually named tumor-associated fibroblasts (TAF)] can exert immunosuppressive effects on T and natural killer (NK) lymphocytes, favoring immune escape. We have analyzed this mechanism colorectal carcinoma (CRC) found that co-culture of NK with TAF prevent IL-2-mediated NKG2D upregulation. This leads to impairment NKG2D-mediated recognition CRC cells, sparing cell activation through DNAM1 or FcγRIIIA (CD16). In situ,...
We describe a simple tetra-primer amplification refractory mutation system PCR (T-ARMS-PCR) for detecting MUTYH mutations, which are associated with colorectal adenomas and cancer.We designed specific T-ARMS-PCR assays 6 mutations (Y165C, G382D, 1395_7delGGA, Y90X, 1103delC, R231H) selected on the basis of frequency their occurrence. also set 3 multiplex T-ARMS assays, each detection 2 mutations. tested DNA samples from patients attenuated or classic adenomatous polyposis coli no detectable...
KIR3DL1 is a natural killer (NK) cell receptor that recognizes the Bw4 epitope of human leukocyte antigen (HLA) class I molecules. Following hematopoietic stem transplantation for patients lacking Bw4, KIR3DL1‐expressing NK cells from Bw4‐positive donors can be alloreactive and eliminate tumor cells. However, alleles having T instead C at nucleotide 320 (encoding leucine 86 serine 86) are not expressed on surface. Thus, all individuals testing positive optimal Bw4‐negative recipients....
Studies on the durability of an intensive, structured education protocol best insulin injection practice are missing for people with type 2 diabetes mellitus (T2DM). The aim this study was to assess education-based rehabilitation in well-trained subjects from our previous multimedia intervention registered as ISTERP-1 study. A total 158 T2DM group 6-month-long study, all whom had successfully attained lower glucose levels compared baseline daily doses and less frequent severe hypoglycemic...
In the few last years, nanosystems have emerged as a potential therapeutic approach to improve efficacy and selectivity of many drugs. Cyclodextrins (CyDs) their nanoparticles been widely investigated drug delivery systems. The covalent functionalization CyD polymer with targeting molecules can this family nanosystems. study, we cross-linked γ- β-cyclodextrin polymers carriers for doxorubicin (ox) oxaliplatin (Oxa). We also functionalized γ-CyD bearing COOH functionalities...
NK cells are innate lymphocytes characterized by the expression of nuclear factor interleukin 3 regulated (NFIL3 or E4BP4), eomesodermin (EOMES) transcription factors (TFs), and ability to exert cytolytic activity release IFN-γ. In haploidentical-hematopoietic stem cell transplantation (haplo-HSCT) setting, CD34(+) donor derived play a major role in control leukemic relapses. Therefore, it is important better define cytokines that influence NK-cell differentiation from precursors. We...