A5002370279- Muscle Physiology and Disorders
- Neuroendocrine regulation and behavior
- Retinoids in leukemia and cellular processes
- Exercise and Physiological Responses
- Nutrition and Health in Aging
- Adipose Tissue and Metabolism
- Histone Deacetylase Inhibitors Research
- Genetics, Aging, and Longevity in Model Organisms
- Protein Kinase Regulation and GTPase Signaling
- Tissue Engineering and Regenerative Medicine
- Anesthesia and Neurotoxicity Research
- Electrospun Nanofibers in Biomedical Applications
- Muscle metabolism and nutrition
- Antioxidant Activity and Oxidative Stress
- Ubiquitin and proteasome pathways
- Heat shock proteins research
- Autophagy in Disease and Therapy
- Neurogenetic and Muscular Disorders Research
- Genetics and Physical Performance
- Acute Myeloid Leukemia Research
- Neuroscience of respiration and sleep
- Protein Hydrolysis and Bioactive Peptides
- Pluripotent Stem Cells Research
- Ion channel regulation and function
- Signaling Pathways in Disease
Sapienza University of Rome
2013-2022
Sorbonne Université
2014-2022
Adaptation Biologique et Vieillissement
2019-2022
Centre National de la Recherche Scientifique
2022
Institut de Biologie Paris-Seine
2022
Inserm
2019-2022
Institut de Myologie
2007-2018
The Ohio State University
2016
IRCCS Humanitas Research Hospital
2016
University of Palermo
2016
Cachexia is a debilitating condition characterized by extreme skeletal muscle wasting that contributes significantly to morbidity and mortality. Efforts elucidate the underlying mechanisms of loss have predominantly focused on events intrinsic myofiber. In contrast, less regard has been given potential contributory factors outside fiber within microenvironment. tumor-bearing mice patients with pancreatic cancer, we found cachexia was associated type damage resulting in activation both...
Skeletal muscle atrophy occurs during disuse and aging, or as a consequence of chronic diseases such cancer diabetes. It is characterized by progressive loss tissue due to hypotrophic changes, degeneration, an inability the regeneration machinery replace damaged myofibers. Tumor necrosis factor (TNF) proinflammatory cytokine known mediate in many inhibit skeletal regeneration. In this study, we investigated role Arg-vasopressin-(AVP-)dependent pathways muscles which was induced local...
Abstract Recent studies have correlated physical activity with a better prognosis in cachectic patients, although the underlying mechanisms are not yet understood. In order to identify pathways involved activity-mediated rescue of skeletal muscle mass and function, we investigated effects voluntary exercise on cachexia colon carcinoma (C26)-bearing mice. Voluntary prevented loss ultimately increasing survival C26-bearing We found that autophagic flux is overloaded both murine models but...
The majority of cancer patients experience dramatic weight loss, due to cachexia and consisting skeletal muscle fat tissue wasting. Cachexia is a negative prognostic factor, interferes with therapy worsens the patients' quality life by affecting function. Mice bearing ectopically-implanted C26 colon carcinoma are widely used as an experimental model cachexia. As part search for novel clinical basic research applications this model, we characterized cellular molecular features C26-bearing...
Chronic disease states are associated with elevated levels of inflammatory cytokines that have been demonstrated to lead severe muscle wasting. A mechanistic understanding wasting is hampered by limited in vivo cytokine models which can be applied emerging mouse mutants as they generated. We developed a simple and novel approach induce adult skeletal based on direct gene transfer an expression vector encoding the secreted form murine tumor necrosis factor-alpha (mTNFalpha). This procedure...
Abstract Heat shock protein 60 (Hsp60) is a chaperone localizing in skeletal muscle mitochondria, whose role poorly understood. In the present study, levels of Hsp60 fibres entire posterior group hindlimb muscles ( gastrocnemius , soleus and plantaris ) were evaluated mice after completing 6-week endurance training program. The correlation between expression four isoforms peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC1α) investigated only . Short-term...
Cultured, spontaneously transformed mouse fibroblasts (Balb/3T12-3 cells) were readily detached from the dish surface in an EDTA-mediated detachment assay. Retinoic acid-treated cells displayed increased adhesion to culture surface. The effect of retinoic acid on Balb/3T12-3 was dose-dependent range 0.05–5 μg/ml (0.17–17 μM) In assay performed cultured for 3 days presence retinoid. earliest detected at 2 17 μM acid. increase by rapidly lost upon removal retinold medium. Synthetic retinoids...
Emerging evidence suggests that the muscle microenvironment plays a prominent role in cancer cachexia. We recently showed NF‐kB‐induced Pax7 overexpression impairs myogenic potential of precursors cachectic mice, suggesting lowering expression may be beneficial evaluated regenerative after acute injury C26 colon carcinoma tumor‐bearing mice and healthy controls. Our analyses confirmed delayed regeneration observed muscles form was associated with persistent local inflammation overexpression....
The multipotency of scaffolds is a new concept. Skeletal muscle acellular (MAS) implanted at the interface Tibialis Anterior/tibial bone and masseter muscle/mandible in murine model were colonized by cells near host bone-cartilaginous tissues bone, thus highlighting importance environment directing cell homing differentiation. These results unveil MAS point to potential this technique as valuable tool musculo-skeletal tissue regeneration.
Histone deacetylase 4 (HDAC4) has been proposed as a target for Amyotrophic Lateral Sclerosis (ALS) because it mediates nerve-skeletal muscle interaction and since its expression in skeletal correlates with the severity of disease. However, our recent studies on response upon long-term denervation highlighted importance HDAC4 maintaining integrity.To fully identify yet uncharacterized functions ALS, we genetically deleted muscles mouse model ALS. Body weight, muscle, innervation spinal cord...
Abstract Static magnetic field (SMF) interacts with mammal skeletal muscle; however, SMF effects on muscle cells are poorly investigated. The myogenic cell line L6, an in vitro model of development, was used to investigate the effect a 80 ± mT generated by custom‐made magnet. promoted differentiation and hypertrophy, i.e., increased accumulation actin myosin formation large multinucleated myotubes. elevated number nuclei per myotube derived from fusion efficiency, no changes proliferation...
Abstract Skeletal muscle is susceptible to injury following trauma, neurological dysfunction, and genetic diseases. homeostasis maintained by a pronounced regenerative capacity, which includes the recruitment of stem cells. Chronic exposure tumor necrosis factor-α (TNF) triggers wasting reminiscent cachexia. To better understand effects TNF upon cells, we exposed injured at specific time points during regeneration. delayed appearance regenerating fibers, without exacerbating fiber death...
Muscle homeostasis involves de novo myogenesis, as observed in conditions of acute or chronic muscle damage. Tumor Necrosis Factor (TNF) triggers skeletal wasting several pathological and inhibits regeneration. We show that intramuscular treatment with the myogenic factor Arg(8)-vasopressin (AVP) enhanced regeneration rescued inhibitory effects TNF on The functional analysis regenerating performance following AVP treatments revealed these factors exerted opposite function. Principal...
The extracellular matrix (ECM) of decellularized organs possesses the characteristics ideal tissue-engineering scaffold (i.e. histocompatibility, porosity, degradability, non-toxicity). We previously observed that muscle acellular (MAS) is a pro-myogenic environment in vivo. In order to determine whether MAS, which basically ECM, behaves as myogenic environment, regardless its location, we analysed MAS interaction with both and nonmuscle cells tissues, assess effects on cell differentiation....
The development of therapeutic strategies for skeletal muscle diseases, such as physical injuries and myopathies, depends on the knowledge regulatory signals that control myogenic process. obestatin/GPR39 system operates an autocrine signal in regulation myogenesis. Using a mouse model regeneration after injury several cellular strategies, we explored potential use obestatin agent treatment trauma-induced injuries. Our results evidenced overexpression preproghrelin, thus obestatin, GPR39...