A5014042288- Heat shock proteins research
- Endoplasmic Reticulum Stress and Disease
- Bacteriophages and microbial interactions
- Enzyme Structure and Function
- Anaerobic Digestion and Biogas Production
- Protein Structure and Dynamics
- thermodynamics and calorimetric analyses
- Monoclonal and Polyclonal Antibodies Research
- Protein purification and stability
- Toxin Mechanisms and Immunotoxins
- Microbial metabolism and enzyme function
- Computational Drug Discovery Methods
- Biofuel production and bioconversion
- Bacterial Genetics and Biotechnology
- Genomics and Phylogenetic Studies
- Extracellular vesicles in disease
- Viral gastroenteritis research and epidemiology
- Gut microbiota and health
- Microbial Community Ecology and Physiology
- RNA and protein synthesis mechanisms
- Erythrocyte Function and Pathophysiology
- Hereditary Neurological Disorders
- Escherichia coli research studies
- Mitochondrial Function and Pathology
- Glycosylation and Glycoproteins Research
University of Maryland, Baltimore
2015-2024
Istituto Euro Mediterraneo di Scienza e Tecnologia
2012-2024
Thomas College
2022
Columbus Center
2010-2020
Accreditation Board for Engineering and Technology
2016-2017
University of Mary
2011
Gruppo Italiano per il Trapianto di Midollo Osseo
2011
Ministério da Saúde
2011
Biotechnology Institute
2006-2010
University of Maryland, Baltimore County
2009
Methanogenesis, the biological production of methane, plays a pivotal role in global carbon cycle and contributes significantly to warming. The majority methane nature is derived from acetate. Here we report complete genome sequence an acetate-utilizing methanogen, Methanosarcina acetivorans C2A. Methanosarcineae are most metabolically diverse methanogens, thrive broad range environments, unique among Archaea forming complex multicellular structures. This diversity reflected M. . At...
Abstract Background Hsp70 chaperones are required for key cellular processes and response to environmental changes survival but they have not been fully characterized yet. The human hsp70 -gene family has an unknown number of members (eleven counted over ten years ago); some described the information is incomplete inconsistent. A coherent body knowledge encompassing all components that would facilitate their study individually as a group lacking. Nowadays, chaperone genes benefits from...
Fecal specimens from nine adults were examined for the presence of methanogenic bacteria. Enrichment cultures five produced methane in 5 days. Of these specimens, three tested and during a short-term incubation. Four did not produce either incubation or enrichment culture. Each culture contained methanogens similar morphology to organisms genus Methanobrevibacter showed factor-420 fluorescence by microscopy. Pure obtained four cultures. isolate grew formed H2-CO2 formate, but growth with...
Using a combination of 16S rRNA analysis and antigenic fingerprinting consisting new published data, the phylogenetic position 18 thermophilic isolates currently classified as Methanobacterium species was reinvestigated. The results were verified by independent methods, including, where applicable, plasmid phage typing. Comparative data for 30 strains belonging to order Methanobacteriales strongly suggested that mesophilic are distantly related should be assigned separate genera. For genus...
Methanogenium frigidum sp. nov. was isolated from the perennially cold, anoxic hypolimnion of Ace Lake in Vesfold Hills Antarctica. The cells were psychrophilic, exhibiting most rapid growth at 15 degrees C and no temperatures above 18 to 20 C. irregular, nonmotile coccoids (diameter, 1.2 2.5 microns) that occurred singly grew by CO2 reduction using H2 as a reductant. Formate could replace H2, but slower. Acetate, methanol, trimethylamine not catabolized. Cells with acetate only organic...
The genome sequence of the genetically tractable, mesophilic, hydrogenotrophic methanogen Methanococcus maripaludis contains 1,722 protein-coding genes in a single circular chromosome 1,661,137 bp. Of (open reading frames [ORFs]), 44% were assigned function, 48% conserved but had unknown or uncertain functions, and 7.5% (129 ORFs) unique to M. maripaludis. ORFs, 27 confirmed encode proteins by mass spectrometric identification peptides. Genes for most known functions pathways identified. For...
Background Hsp60, a Group I mitochondrial chaperonin, is classically considered an intracellular chaperone with residence in the mitochondria; nonetheless, last few years it has been found extracellularly as well cell membrane. Important questions remain pertaining to extracellular Hsp60 such how generalized its occurrence outside cells, what are functions and translocation mechanisms that transport of cell. These particularly relevant for cancer biology since believed chaperones, like...
BACKGROUND Heat shock protein 60 (Hsp60) is a chaperonin involved in tumorigenesis, but its participation tumor development and progression not well understood value as biomarker has been fully elucidated. In the current study, authors presented evidence supporting theory that Hsp60 potential therapeutic target patients with large bowel cancer. METHODS The studied population of 97 subjects, including controls. Immunomorphology, Western blot analysis, quantitative real‐time polymerase chain...
Background In a previous work we showed for the first time that human tumor cells secrete Hsp60 via exosomes, which are considered immunologically active microvesicles involved in progression. This finding raised questions concerning route followed by to reach its location them, and whether can be secreted also other mechanisms, e.g., Golgi. We addressed these issues presented here. Principal Findings found localizes cell plasma membrane, is associated with lipid rafts, ends up exosomal...
Severe acute respiratory syndrome corona virus 2 (SARS-CoV-2), the cause of COVID-19 disease, has potential to elicit autoimmunity because mimicry human molecular chaperones by viral proteins. We compared proteins with chaperones, many which are heat shock proteins, determine if they share amino acid-sequence segments immunogenic-antigenic potential, can cross-reactive antibodies and effector immune cells capacity damage-destroy a mechanism autoimmunity. identified that putatively...
Samples of human dental plaque were examined for the presence methanogenic bacteria. Of 54 samples from 36 patients, 20 yielded H2/CO2-using enrichment cultures. All methanogen-positive patients with some degree periodontal disease. The predominant populations in enrichments had morphologies characteristic Methanobrevibacter spp. In six derived three common methanogen was antigenically similar to smithii. same true isolates obtained axenic culture a fourth patient. and two closer strain ALI...
Twelve vaginal samples were collected from separate patients, processed anaerobically, and added to methanogenic enrichment medium. Methanogenic activity was detected in two samples, both of which patients with bacterial vaginosis. None the healthy yielded positive methanogen cultures. One sample a patient vaginosis did not show any detectable activity. Two isolates obtained one methanogen-positive identified as Methanobrevibacter smithii on basis morphological, cultural, immunological features.