A5100756193- Methane Hydrates and Related Phenomena
- Microbial Community Ecology and Physiology
- Crystallization and Solubility Studies
- Metalloenzymes and iron-sulfur proteins
- X-ray Diffraction in Crystallography
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Anaerobic Digestion and Biogas Production
- Genomics and Phylogenetic Studies
- Enzyme Structure and Function
- Biofuel production and bioconversion
- Probiotics and Fermented Foods
- Microbial Metabolic Engineering and Bioproduction
- Microbial metabolism and enzyme function
- Polar Research and Ecology
- Chromatography in Natural Products
- Biochemical and Molecular Research
- Plant-Microbe Interactions and Immunity
- Fungal and yeast genetics research
- Selenium in Biological Systems
- Hydraulic Fracturing and Reservoir Analysis
- Polyamine Metabolism and Applications
- Traditional Chinese Medicine Analysis
- Hydrocarbon exploration and reservoir analysis
- Plant Gene Expression Analysis
Zhejiang A & F University
2024
University of Maryland, Baltimore
2024
University of Maryland Medical Center
2024
Jianghan University
2024
National Chung Hsing University
2024
Sichuan Agricultural University
2019-2023
Rice Research Institute
2020-2023
Wuhan University of Technology
2023
ExxonMobil (United States)
2021-2022
Queen's University
2022
Methanogenium frigidum sp. nov. was isolated from the perennially cold, anoxic hypolimnion of Ace Lake in Vesfold Hills Antarctica. The cells were psychrophilic, exhibiting most rapid growth at 15 degrees C and no temperatures above 18 to 20 C. irregular, nonmotile coccoids (diameter, 1.2 2.5 microns) that occurred singly grew by CO2 reduction using H2 as a reductant. Formate could replace H2, but slower. Acetate, methanol, trimethylamine not catabolized. Cells with acetate only organic...
Broad-spectrum resistance is highly preferred in crop breeding programmes. Previously, we have reported the identification of broad-spectrum resistance-Digu 1 (bsr-d1) allele from rice Digu. The bsr-d1 prevents activation Bsr-d1 expression by Magnaporthe oryzae infection and degradation H2 O2 peroxidases, leading to M. oryzae. However, it remains unknown whether defence pathways other than burst peroxidases contribute bsr-d1-mediated immunity. Blast was determined leaves spray punch...
Three multiprotein systems are known for iron-sulfur (Fe-S) cluster biogenesis in prokaryotes and eukaryotes as follows: the NIF (nitrogen fixation), ISC (iron-sulfur cluster), SUF (mobilization of sulfur) systems. In all three, cysteine is physiological sulfur source, transferred from desulfurase through a persulfidic intermediate to scaffold protein. However, biochemical nature source Fe-S assembly archaea unknown, many lack homologs desulfurases. Methanococcus maripaludis methanogenic...
The sulfur-containing nucleosides in transfer RNA (tRNAs) are present all three domains of life; they have critical functions for accurate and efficient translation, such as tRNA structure stabilization proper codon recognition. modification enzymes ThiI (in bacteria archaea) Ncs6 archaea eukaryotic cytosols) catalyze the formation 4-thiouridine (s4U) 2-thiouridine (s2U), respectively. homologs were proposed to sulfur via cysteine persulfide enzyme adducts, whereas reaction mechanism remains...
Selenocysteine (Sec) is naturally co-translationally incorporated into proteins by recoding the UGA opal codon with a specialized elongation factor (SelB in bacteria) and an RNA structural signal (SECIS element). We have recently developed SECIS-free selenoprotein synthesis system that site-specifically--using UAG amber codon--inserts Sec depending on Tu (EF-Tu). Here, we describe engineering of EF-Tu for improved synthesis. A Sec-specific selection was established expression human protein...
We aimed to decipher the molecular genetic basis of disease in a cohort children with uniform clinical presentation neonatal irritability, spastic or dystonic quadriplegia, virtually absent psychomotor development, axonal neuropathy, and elevated blood/CSF lactate.We performed whole-exome sequencing blood DNA from index patients. Detected compound heterozygous mutations were confirmed by Sanger sequencing. Structural predictions bacterial activity assay evaluate functional consequences...
Continuous culture, transcriptome arrays, and measurements of cellular amino acid pools tRNA charging levels were used to determine the response Methanococcus maripaludis leucine limitation. For comparison, responses phosphate H2 limitations measured as well. In addition, effect growth rate was determined. Leucine limitation resulted in a broad response. tRNA(Leu) decreased, but only small increases mRNA seen for biosynthesis genes. However, free isoleucine valine showed significant...
4-Thiouridine (s(4)U) is a conserved modified nucleotide at position 8 of bacterial and archaeal tRNAs plays role in protecting cells from near-UV killing. Escherichia coli employs the following two enzymes for its synthesis: cysteine desulfurase IscS, which forms Cys persulfide enzyme adduct free Cys; ThiI, adenylates U8 transfers sulfur IscS to form s(4)U. The C-terminal rhodanese-like domain (RLD) ThiI responsible sulfurtransferase activity. mechanism s(4)U biosynthesis archaea not known...
Abstract Methanogens and anaerobic methane-oxidizing archaea (ANME) are important players in the global carbon cycle. Methyl-coenzyme M reductase (MCR) is a key enzyme methane metabolism, catalyzing last step methanogenesis first oxidation. Divergent mcr -like genes have recently been identified uncultured archaeal lineages. However, assembly biochemistry of MCRs from remain largely unknown. Here we present an approach to study by heterologous expression methanogen, Methanococcus maripaludis...
Fungal pathogens typically use secreted effector proteins to suppress host immune activators facilitate invasion. However, there is rarely evidence supporting the idea that fungal secretory contribute pathogenesis by transactivating genes defense. We previously found pathogen Magnaporthe oryzae induces rice Bsr-d1 infection and hypothesized a mediates this induction. Here, we report MoSPAB1 M. directly binds promoter induce its expression, facilitating pathogenesis. Amino acids 103-123 of...
The impact of leptin resistance on intestinal mucosal barrier integrity, appetite regulation, and hepatic lipid metabolism through the microbiota–gut–brain–liver axis has yet to be determined. Water extract Phyllanthus emblica L. fruit (WEPE) its bioactive compound gallic acid (GA) effectively alleviated methylglyoxal (MG)-triggered in vitro. Therefore, this study investigated how WEPE GA intervention relieve resistance-associated dysfunction mucosa, appetite, accumulation high-fat diet...
The Methanococcus maripaludis energy-conserving hydrogenase B (Ehb) generates low potential electrons required for autotrophic CO(2) assimilation. To analyze the importance of individual subunits in Ehb structure and function, markerless in-frame deletions were constructed a number M. ehb genes. These genes encode large small (ehbN ehbM, respectively), polyferredoxin ferredoxin (ehbK ehbL, an ion translocator (ehbF). In addition, gene replacement mutation was encoding putative...
The proteolytic bacterium strain A7P-90mT was isolated from Lake Untersee, Antarctica. anoxic water collected a perennially sealed (~100 millennia) glacial ice lake. Gram-stain-negative cells were 0.18-0.3×8.0-25.0 µm in size, straight, slender rods with unusual gliding motility by external, not previously reported, organelles named here as antiae. At the end of stationary phase growth, spheroplasts terminally formed and resembled dandelions. After death, helical. isolate an athalassic,...
A Gram-stain-positive bacterium, strain LZ-22T, was isolated from a rhizosphere of moss Leptobryum sp. collected at the shore Lake Zub in Antarctica. Cells were motile, straight or pleomorphic rods with sizes 0.6-1.0×3.5-10 µm. The novel isolate facultatively anaerobic, catalase-positive, psychrotolerant mesophile. Growth observed 3-41 °C (optimum 24-28 °C), 0-7 % (w/v) NaCl 0.25 %) and pH 4.0-9.0 7.8). quinone system LZ-22T possessed predominately menaquinone MK-9(H4). genomic G+C content...
Methanococcus is a genus of well-characterized mesophilic and hydrogenotrophic methanogens with robust genetic system. Commonly found in tidal marshes, they compete sulfate- iron-reducing bacteria for hydrogen gas (H2) formate, their two major energy sources. They are characterized by rapid growth mineral medium requirements marine levels salts optimal growth. Genetic tools developed over the last twenty years include improvements shuttle vectors production recombinant proteins CRISPR...
Methanococcus maripaludis is a rapidly growing, hydrogenotrophic, and genetically tractable methanogen with unique capabilities to convert formate CO2 CH4. The existence of genome-scale metabolic models an established, robust system for both large-scale continuous cultivation make it amenable industrial applications. However, the lack molecular tools differential gene expression has hindered its application as microbial cell factory produce biocatalysts biochemicals. In this study, library...
The pathway of lysine biosynthesis in the methanococci has not been identified previously. A variant diaminopimelic acid (DAP) uses diaminopimelate aminotransferase (DapL) to catalyze direct conversion tetrahydrodipicolinate (THDPA) ll-DAP. Recently, enzyme DapL (MTH52) was Methanothermobacter thermautotrophicus and shown belong DapL1 group. Although Methanococcus maripaludis genome lacks a gene that can be unambiguously assigned function based on sequence similarity, open reading frame...