A5101441917- Plant-Microbe Interactions and Immunity
- Plant Disease Resistance and Genetics
- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Legume Nitrogen Fixing Symbiosis
- Plant pathogens and resistance mechanisms
- Plant Pathogenic Bacteria Studies
- Rice Cultivation and Yield Improvement
- Plant Gene Expression Analysis
- Plant Reproductive Biology
- Genetic Mapping and Diversity in Plants and Animals
- Plant Pathogens and Fungal Diseases
- Photosynthetic Processes and Mechanisms
- Plant responses to water stress
- Plant tissue culture and regeneration
- Plant Genetic and Mutation Studies
- Biofuel production and bioconversion
- Silicon Effects in Agriculture
- Nematode management and characterization studies
- Semiconductor materials and interfaces
- Circular RNAs in diseases
- Polysaccharides and Plant Cell Walls
- Mycotoxins in Agriculture and Food
- Plant Virus Research Studies
- Phytoplasmas and Hemiptera pathogens
Rice Research Institute
2015-2024
Sichuan Agricultural University
2015-2024
Summary mi RNA s contribute to plant resistance against pathogens. Previously, we found that the function of miR398b in immunity rice differs from Arabidopsis. However, underlying mechanisms are unclear. In this study, characterized mutants target genes and demonstrated multiple superoxide dismutase miR398b‐regulated blast fungus Magnaporthe oryzae . Out four miR398b, mutations Cu/Zn‐Superoxidase Dismutase1 ( CSD 1 ), 2 Os11g09780 Superoxide DismutaseX , SODX ) led enhanced M. increased...
Fitness cost is a common phenomenon in rice blast disease-resistance breeding. MiR396 highly conserved microRNA (miRNA) family targeting Growth Regulating Factor (OsGRF) genes. Mutation at the target site of miR396 certain OsGRF gene or blocking expression leads to increased grain yield. Here we demonstrated that fitness can be trade-off miR396-OsGRFs module via balancing growth and immunity against fungus. The accumulation isoforms was significantly susceptible accession, but fluctuated...
Circular RNAs (circRNAs) play roles in various biological processes, but their functions the rice (Oryza sativa) response to Magnaporthe oryzae remain elusive. Here, we demonstrate that circRNAs are involved rice-M. interaction using comparative circRNA-sequencing and transgenic approaches. We identified 2932 high-confidence from young leaves of blast-resistant accession International Rice Blast Line Pyricularia-Kanto51-m-Tsuyuake (IR25) blast-susceptible Lijiangxin Tuan Heigu (LTH) under...
Reactive oxygen species (ROS) act as a group of signaling molecules in rice functioning regulation development and stress responses. Respiratory burst oxidase homologues (Rbohs) are key enzymes generation ROS. However, the role nine Rboh family members was not fully understood multiple disease resistance yield traits. In this study, we constructed mutants each genes detected their requirement Our results revealed that mutations five (RbohA, RbohB, RbohE, RbohH, RbohI) lead to compromised...
Although plant-specific NAC transcription factors play crucial roles in response to abiotic stress, few reports describe the regulation of genes maize (Zea mays) by cis-natural antisense transcripts (cis-NATs). In this study, 521 from Gramineae were classified, which 51 contained cis-NATs. ZmNAC48 and cis-NATZmNAC48 co-localized same cell nucleus, both responded drought stress. Arabidopsis plants overexpressing had improved tolerance, lower rate water loss, enhanced stomatal closure, higher...
Grain formation is fundamental for crop yield but vulnerable to abiotic and biotic stresses. Rice grain production threatened by the false smut fungus Ustilaginoidea virens, which specifically infects rice floral organs, disrupting fertilization seed formation. However, little known about molecular mechanisms of U. virens–rice interaction genetic basis resistance. Here, we report that virens secretes a cytoplasmic effector, UvCBP1, facilitate infection flowers. Mechanistically, UvCBP1...
Abstract The flower-infecting fungus Ustilaginoidea virens causes rice false smut, which is a severe emerging disease threatening (Oryza sativa) production worldwide. False smut not only reduces yield, but more importantly produces toxins on grains, posing great threat to food safety. U. invades spikelets via the gap between 2 bracts (lemma and palea) enclosing floret specifically infects stamen pistil. Molecular mechanisms for virens–rice interaction are largely unknown. Here, we...
Summary Rice false smut has emerged as a serious grain disease in rice production worldwide. The is characterized by the transformation of individual florets into balls, which caused fungal pathogen Ustilaginoidea virens . To date, little known about host factors required for ball formation U In this study, we identified histological determinants balls inoculating floral mutants defective with respect to parts. results showed that could form mature pistils, but failed develop superwoman...
MicroRNAs (miRNAs) play vital roles in plant development and defence responses against various stresses. Here, we show that blocking miR1871 improves rice resistance Magnaporthe oryzae enhances grain yield simultaneously. The transgenic lines overexpressing (OX1871) exhibit compromised resistance, suppressed reduced panicle number resulting slightly decreased yield. In contrast, the (MIM1871) improved enhanced significantly increased leading to per plant. RNA-seq assay response assays reveal...
microRNAs act as fine-tuners in the regulation of plant growth and resistance against biotic abiotic stress. Here we demonstrate that rice miR1432 fine-tunes yield blast disease via different modules. Overexpression leads to compromised decreased yield, whereas blocking using a target mimic results enhanced yield. suppresses expression LOC_Os03g59790, which encodes an EF-hand family protein 1 (OsEFH1). OsEFH1 but grain Further study revealed are differentially responsive chitin,...
Rice production is threatened by multiple pathogens. Breeding cultivars with broad-spectrum disease resistance necessary to maintain and improve crop production. Previously we found that overexpression of miR160a enhanced rice blast resistance. However, it unclear whether also regulates against other pathogens, what the downstream signaling pathways are. Here, demonstrate positively causative agents blast, leaf blight sheath in rice. Mutations miR160a-targeted Auxin Response Factors result...
Magnaporthe oryzae is the causative agent of rice blast, a devastating disease in worldwide. Based on gene-for-gene paradigm, resistance (R) proteins can recognize their cognate avirulence (AVR) effectors to activate effector-triggered immunity. AVR genes have been demonstrated evolve rapidly, leading breakdown genes. Therefore, understanding variation essential deployment resistant cultivars harboring R In this study, we analyzed nucleotide sequence polymorphisms eight known genes, namely,...
Fungal pathogens typically use secreted effector proteins to suppress host immune activators facilitate invasion. However, there is rarely evidence supporting the idea that fungal secretory contribute pathogenesis by transactivating genes defense. We previously found pathogen Magnaporthe oryzae induces rice Bsr-d1 infection and hypothesized a mediates this induction. Here, we report MoSPAB1 M. directly binds promoter induce its expression, facilitating pathogenesis. Amino acids 103-123 of...
MicroRNAs fine-tune plant growth and resistance against multiple biotic abiotic stresses. The trade-off between biomass can penalize crop yield. In this study, we have shown that rice miR530 regulates blast disease resistance, yield, period. While the overexpression of results in compromised reduced grain late maturity, blocking using a target mimic (MIM530) leads to enhanced increased early maturity. Further study revealed accumulation was decreased both leaves panicles along with increase...
False smut is a destructive grain disease of rice worldwide, characterized by false balls formed in flowers. Here we identified small secreted protein UV_1261 contributing to virulence Ustilaginoidea virens, the causal agent this disease. The sequence was highly conserved among isolates U. virens and absent other fungi. encodes targeted plant chloroplasts. Its expression exhibited bimodal pattern during pathogenesis. Ectopic Nicotiana benthamiana Arabidopsis led suppression flg22-induced ROS...
Arabidopsis RESISTANCE TO POWDERY MILDEW 8.1 (RPW8.1) is an important tool for engineering broad-spectrum disease resistance against multiple pathogens. Ectopic expression of RPW8.1 leads to enhanced with cell death at leaves and compromised plant growth, implying a regulatory mechanism balancing RPW8.1-mediated growth. Here, we show that constitutively enhances the transcription factor WRKY51 activates salicylic acid ethylene signalling pathways; in turn suppresses expression, forming...
Ustilaginoidea virens is a biotrophic fungal pathogen specifically colonizing rice floral organ and causes false smut disease of rice. This has emerged as serious problem that hinders the application high-yield cultivars, by reducing grain yield quality well introducing mycotoxins. However, pathogenic mechanisms U. are still enigmatic. Here we demonstrate employs secreted protein UvCBP1 to manipulate plant immunity. In planta expression led compromised chitin-induced defense responses in...
Flower opening and stigma exertion are two critical traits for cross-pollination during seed production of hybrid rice (Oryza sativa L.). In this study, we demonstrate that the miR167d-ARFs module regulates size flower is associated with elongation stamen filaments cell arrangement lodicules. The overexpression miR167d (OX167d) resulted in failed filaments, increased size, morphological alteration lodicule, resulting cleistogamy. Blocking by target mimicry also led to a individual floral...
Summary Arabidopsis RESISTANCE TO POWDERY MILDEW 8.2 (RPW8.2) is specifically induced by the powdery mildew (PM) fungus ( Golovinomyces cichoracearum ) in infected epidermal cells to activate immunity. However, mechanism of RPW8.2‐induction not well understood. Here, we identify a G. effector that interacts with RPW8.2, named Gc‐RPW8.2 interacting protein 1 (GcR8IP1), yeast two‐hybrid screen an cDNA library. GcR8IP1 physically associated RPW8.2 its REALLY INTERESTING NEW GENE finger domain...
Abstract Flower opening and stigma exertion are two critical traits for cross-pollination during seed production of hybrid rice ( Oryza sativa L.). In this study, we demonstrate that the miR167d- ARF s module regulates size flower is associated with elongation stamen filaments cell arrangement lodicules, resulting in cleistogamy. The overexpression miR167d (OX167d) resulted failed filaments, increased size, morphological alteration lodicule. Blocking miR167 by target mimicry also led to a...