A5086672280- Plant-Microbe Interactions and Immunity
- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Plant Gene Expression Analysis
- Plant Disease Resistance and Genetics
- Legume Nitrogen Fixing Symbiosis
- Plant Reproductive Biology
- Rice Cultivation and Yield Improvement
- Genetic Mapping and Diversity in Plants and Animals
- Plant pathogens and resistance mechanisms
- Plant Pathogenic Bacteria Studies
- Cyclopropane Reaction Mechanisms
- Photosynthetic Processes and Mechanisms
- Polysaccharides and Plant Cell Walls
- Neuropeptides and Animal Physiology
- Monoclonal and Polyclonal Antibodies Research
- Postharvest Quality and Shelf Life Management
- Nematode management and characterization studies
- Catalytic C–H Functionalization Methods
- Plant Virus Research Studies
- Cocoa and Sweet Potato Agronomy
- Silicon Effects in Agriculture
- Wheat and Barley Genetics and Pathology
- Mycotoxins in Agriculture and Food
- Receptor Mechanisms and Signaling
Sichuan Agricultural University
2017-2024
Chengdu University of Traditional Chinese Medicine
2024
Rice Research Institute
2017-2022
Hubei University
2022
Summary mi RNA s contribute to plant resistance against pathogens. Previously, we found that the function of miR398b in immunity rice differs from Arabidopsis. However, underlying mechanisms are unclear. In this study, characterized mutants target genes and demonstrated multiple superoxide dismutase miR398b‐regulated blast fungus Magnaporthe oryzae . Out four miR398b, mutations Cu/Zn‐Superoxidase Dismutase1 ( CSD 1 ), 2 Os11g09780 Superoxide DismutaseX , SODX ) led enhanced M. increased...
Fitness cost is a common phenomenon in rice blast disease-resistance breeding. MiR396 highly conserved microRNA (miRNA) family targeting Growth Regulating Factor (OsGRF) genes. Mutation at the target site of miR396 certain OsGRF gene or blocking expression leads to increased grain yield. Here we demonstrated that fitness can be trade-off miR396-OsGRFs module via balancing growth and immunity against fungus. The accumulation isoforms was significantly susceptible accession, but fluctuated...
Grain formation is fundamental for crop yield but vulnerable to abiotic and biotic stresses. Rice grain production threatened by the false smut fungus Ustilaginoidea virens, which specifically infects rice floral organs, disrupting fertilization seed formation. However, little known about molecular mechanisms of U. virens–rice interaction genetic basis resistance. Here, we report that virens secretes a cytoplasmic effector, UvCBP1, facilitate infection flowers. Mechanistically, UvCBP1...
Abstract The flower-infecting fungus Ustilaginoidea virens causes rice false smut, which is a severe emerging disease threatening (Oryza sativa) production worldwide. False smut not only reduces yield, but more importantly produces toxins on grains, posing great threat to food safety. U. invades spikelets via the gap between 2 bracts (lemma and palea) enclosing floret specifically infects stamen pistil. Molecular mechanisms for virens–rice interaction are largely unknown. Here, we...
MicroRNAs (miRNAs) play vital roles in plant development and defence responses against various stresses. Here, we show that blocking miR1871 improves rice resistance Magnaporthe oryzae enhances grain yield simultaneously. The transgenic lines overexpressing (OX1871) exhibit compromised resistance, suppressed reduced panicle number resulting slightly decreased yield. In contrast, the (MIM1871) improved enhanced significantly increased leading to per plant. RNA-seq assay response assays reveal...
microRNAs act as fine-tuners in the regulation of plant growth and resistance against biotic abiotic stress. Here we demonstrate that rice miR1432 fine-tunes yield blast disease via different modules. Overexpression leads to compromised decreased yield, whereas blocking using a target mimic results enhanced yield. suppresses expression LOC_Os03g59790, which encodes an EF-hand family protein 1 (OsEFH1). OsEFH1 but grain Further study revealed are differentially responsive chitin,...
Rice production is threatened by multiple pathogens. Breeding cultivars with broad-spectrum disease resistance necessary to maintain and improve crop production. Previously we found that overexpression of miR160a enhanced rice blast resistance. However, it unclear whether also regulates against other pathogens, what the downstream signaling pathways are. Here, demonstrate positively causative agents blast, leaf blight sheath in rice. Mutations miR160a-targeted Auxin Response Factors result...
Magnaporthe oryzae is the causative agent of rice blast, a devastating disease in worldwide. Based on gene-for-gene paradigm, resistance (R) proteins can recognize their cognate avirulence (AVR) effectors to activate effector-triggered immunity. AVR genes have been demonstrated evolve rapidly, leading breakdown genes. Therefore, understanding variation essential deployment resistant cultivars harboring R In this study, we analyzed nucleotide sequence polymorphisms eight known genes, namely,...
The Arabidopsis RESISTANCE TO POWDERY MILDEW 8.1 (RPW8.1) activates confined cell death and defense against different pathogens. However, the underlying regulatory mechanisms still remain elusive. Here, we show that RPW8.1 ethylene signaling that, in turn, negatively regulates expression. binds stabilizes 1-aminocyclopropane-1-carboxylate oxidase 4 (ACO4), which may part explain increased production RPW8.1-expressing plants. In return, ACO4 other key components of regulate RPW8.1-mediated...
MicroRNAs fine-tune plant growth and resistance against multiple biotic abiotic stresses. The trade-off between biomass can penalize crop yield. In this study, we have shown that rice miR530 regulates blast disease resistance, yield, period. While the overexpression of results in compromised reduced grain late maturity, blocking using a target mimic (MIM530) leads to enhanced increased early maturity. Further study revealed accumulation was decreased both leaves panicles along with increase...
Arabidopsis RESISTANCE TO POWDERY MILDEW 8.1 (RPW8.1) is an important tool for engineering broad-spectrum disease resistance against multiple pathogens. Ectopic expression of RPW8.1 leads to enhanced with cell death at leaves and compromised plant growth, implying a regulatory mechanism balancing RPW8.1-mediated growth. Here, we show that constitutively enhances the transcription factor WRKY51 activates salicylic acid ethylene signalling pathways; in turn suppresses expression, forming...
Ustilaginoidea virens is a biotrophic fungal pathogen specifically colonizing rice floral organ and causes false smut disease of rice. This has emerged as serious problem that hinders the application high-yield cultivars, by reducing grain yield quality well introducing mycotoxins. However, pathogenic mechanisms U. are still enigmatic. Here we demonstrate employs secreted protein UvCBP1 to manipulate plant immunity. In planta expression led compromised chitin-induced defense responses in...
Flower opening and stigma exertion are two critical traits for cross-pollination during seed production of hybrid rice (Oryza sativa L.). In this study, we demonstrate that the miR167d-ARFs module regulates size flower is associated with elongation stamen filaments cell arrangement lodicules. The overexpression miR167d (OX167d) resulted in failed filaments, increased size, morphological alteration lodicule, resulting cleistogamy. Blocking by target mimicry also led to a individual floral...
We report a catalytic Friedel–Crafts type arylation of donor–acceptor diazo compounds with aniline derivatives under metal-free and mild conditions.
Summary The extrahaustorial membrane (EHM) is a host‐derived interfacial encasing the haustorium of powdery mildew fungi. Arabidopsis thaliana RESISTANCE TO POWDERY MILDEW 8.2 (RPW8.2) specifically targeted to EHM via two EHM‐targeting signals. Here, we demonstrate that proper coordination between trafficking forces engaged signals and nuclear localization (NLSs), as well export (NESs), in RPW8.2 critical for activation cell death defense. We show absence pathogens, partitioned cytoplasm...
Summary Arabidopsis RESISTANCE TO POWDERY MILDEW 8.2 (RPW8.2) is specifically induced by the powdery mildew (PM) fungus ( Golovinomyces cichoracearum ) in infected epidermal cells to activate immunity. However, mechanism of RPW8.2‐induction not well understood. Here, we identify a G. effector that interacts with RPW8.2, named Gc‐RPW8.2 interacting protein 1 (GcR8IP1), yeast two‐hybrid screen an cDNA library. GcR8IP1 physically associated RPW8.2 its REALLY INTERESTING NEW GENE finger domain...
Ectopic expression of the Arabidopsis RESISTANCE TO POWDERY MILDEW8.1 (RPW8.1) boosts pattern-triggered immunity leading to enhanced resistance different pathogens in and rice. However, underlying regulatory mechanism remains largely elusive. Here, we report that XAP5 CIRCADIAN TIMEKEEPER (XCT, At2g21150) positively regulates RPW8.1-mediated cell death disease resistance. Forward genetic screen identified b3-17 mutant exhibited less susceptibility powdery mildew bacterial pathogens....
Abstract Flower opening and stigma exertion are two critical traits for cross-pollination during seed production of hybrid rice ( Oryza sativa L.). In this study, we demonstrate that the miR167d- ARF s module regulates size flower is associated with elongation stamen filaments cell arrangement lodicules, resulting in cleistogamy. The overexpression miR167d (OX167d) resulted failed filaments, increased size, morphological alteration lodicule. Blocking miR167 by target mimicry also led to a...
ABSTRACT Crops with broad-spectrum resistance (BSR) to diseases are highly desirable in agricultural production. Identification of BSR loci and dissection the underlying mechanisms fundamental for crop breeding. Here, we describe identification characterization a rice UMP1 allele, which confers race-nonspecific against blast pathogen Magnaporthe oryzae . OsUMP1 encodes proteasome maturation factor that contributes 26S abundance activity rice. Modulation expression leads proteome changes,...
Abstract microRNAs act as fine-tuners in the regulation of plant growth and resistance against biotic abiotic stress. Here we demonstrate that rice miR1432 fine-tunes yield blast disease via different modules. The expression is differentially regulated susceptible accessions by infection fungus Magnaporthe oryzae . Overexpression leads to compromised decreased yield, whereas blocking using a target mimic results enhanced yield. Moreover, suppresses LOC_Os03g59790 , which encodes an EF-hand...
Abstract RESISTANCE TO POWDERY MILDEW 8 (RPW8) defines a unique N-terminal coiled-coil domain of nucleotide-binding and leucine-rich repeat immune receptors required for signaling in plants. Arabidopsis RPW8.2 is specifically induced by the powdery mildew (PM) fungus ( Golovinomyces cichoracearum ) infected epidermal cells to activate immunity. The mechanism RPW8.2-induction not well understood. Here, we identify G. factor delivered nucleus host cell, named Gc-RPW8.2 interacting protein 1...
ABSTRACT Crop floral diseases are economically important as they reduce grain yield and quality even introduce food toxins. Rice false smut has emerged a serious disease producing mycotoxins. However, very little is known on the interaction mechanisms between rice flower causal fungus Ustilaginoidea virens . Here we show that conserved anti-fungal immunity in disarmed by U. via secreted protein UvChi1. UvChi1 functioned an essential virulence factor directly interacted with chitin receptor...