A5080948539- Photosynthetic Processes and Mechanisms
- Algal biology and biofuel production
- Microbial Community Ecology and Physiology
- Protist diversity and phylogeny
- Metabolomics and Mass Spectrometry Studies
- Crystallization and Solubility Studies
- Analytical Chemistry and Chromatography
- Plant biochemistry and biosynthesis
- Chromatography in Natural Products
- Phytochemicals and Antioxidant Activities
- X-ray Diffraction in Crystallography
- Botanical Research and Chemistry
- Plant Gene Expression Analysis
- Advanced Proteomics Techniques and Applications
- Marine and coastal ecosystems
- Biocrusts and Microbial Ecology
- Mass Spectrometry Techniques and Applications
- Mitochondrial Function and Pathology
- Plant tissue culture and regeneration
- Diatoms and Algae Research
- Endoplasmic Reticulum Stress and Disease
- Bioactive natural compounds
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Ginseng Biological Effects and Applications
- Plant Ecology and Soil Science
University of Turku
2014-2024
Siemens Healthcare (Germany)
2019
Åbo Akademi University
2014-2015
Turku Centre for Biotechnology
2014-2015
Pediatrics and Genetics
2015
Yale University
2015
Imaging Center
2015
Institute for Molecular Medicine Finland
2015
University of Amsterdam
2015
University of Helsinki
2015
Abstract Flavonoid conjugates constitute several classes of plant phenolic secondary metabolites including many isomeric compounds differing in the hydroxylation pattern and substitution their rings with different groups such as alkyls, acyls or sugars. These occur tissues mainly glycosides cases it is necessary to have reliable detailed information concerning structure these natural products. Our results were obtained using leaf extracts Arabidopsis thaliana Lupinus angustifolius which...
• In a phenotypic screen of plants constitutively overexpressing DOF (DNA-binding-with-one-finger) transcription factors under the control Cauliflower mosaic virus 35S promoter, AtDOF4;2 was identified as gene inducing bushy plant phenotype and potentially being involved in regulation phenylpropanoid metabolism Arabidopsis. Further molecular biochemical characterization performed parallel using transgenic with enhanced reduced expression. The expression pattern determined by quantitative...
We aimed to decipher the molecular genetic basis of disease in a cohort children with uniform clinical presentation neonatal irritability, spastic or dystonic quadriplegia, virtually absent psychomotor development, axonal neuropathy, and elevated blood/CSF lactate.We performed whole-exome sequencing blood DNA from index patients. Detected compound heterozygous mutations were confirmed by Sanger sequencing. Structural predictions bacterial activity assay evaluate functional consequences...
Summary Organellar reactive oxygen species ( ROS ) signalling is a key mechanism that promotes the onset of defensive measures in stress‐exposed plants. The underlying molecular mechanisms and feedback regulation loops, however, still remain poorly understood. Our previous work has shown specific regulatory B′γ subunit protein phosphatase 2A PP 2A) required to control organellar associated metabolic adjustments Arabidopsis thaliana . Here, we addressed through which 2A‐B′γ impacts on...
Plant interactions with environmental factors cause changes in the metabolism and regulation of biochemical physiological processes. defense against pathogenic microorganisms depends on an innate immunity system that is activated as a result infection. There are two mechanisms triggering this system: basal perception microbe-associated molecular patterns through pattern recognition receptors situated cell surface effector-triggered (ETI). An induced biosynthesis bioactive secondary...
The flavodiiron proteins (FDPs) Flv1 and Flv3 in cyanobacteria function photoreduction of O2 to H2O, without concomitant formation reactive oxygen species, known as the Mehler-like reaction. Both are essential for growth under fluctuating light (FL) intensities, providing protection PSI. Here we compared global transcript profiles wild type (WT), Δflv1 Δflv1/Δflv3 grown constant (GL) FL. In WT, FL induced largest down-regulation transcripts involved carbon-concentrating mechanisms (CCMs),...
O-Phosphorylation has been shown in photosynthesis-related proteins a cyanobacterium Synechocystis sp. strain PCC 6803 (thereafter 6803), suggesting that phosphorylation of S, T, and Y residues might be important processes. Investigation biological roles these events requires confident knowledge the phosphorylated sites prospects for their individual assessment. We performed phosphoproteomic analysis using TiO2 enrichment phosphopeptides, followed by LC–MS/MS, discovered 367 190...
Hairy roots and suspension cell cultures are commonly used in deciphering different problems related to the biochemistry physiology of plant secondary metabolites. Here, we address about issue possible differences profiles flavonoid compounds their glycoconjugates derived from various materials grown a standard culture media. We compared flavonoids isolated seedling roots, hairy root model legume plant, Medicago truncatula. The analyses were conducted with isolates as well LC/MS target...
Metabolite fingerprinting is widely used to unravel the chemical characteristics of biological samples. Multivariate data analysis and other statistical tools are subsequently analyze visualize plasticity metabolome and/or relationship between those However, there limitations these approaches for example because multi-dimensionality that makes interpretation obtained from untargeted almost impossible an average human being. These make information prime importance in studies be partially...
Abstract Introduction: Glycosylation at different hydroxyl groups of flavonoids and acylation sugar moieties are ubiquitous modifications observed in plants. These give rise to simultaneous presence numerous isomeric isobaric compounds tissues extracts thereof. Objective: To develop UPLC‐MS method capable for resolution malonylated glycoconjugates recognition structural differences. Methodology: Flavonoid were extracted from leaves blue lupin ( Lupinus angustifolius L.) plants with 80%...
The cyanobacterium Synechocystis sp. PCC 6803 (S. 6803) is a well-established model species in oxygenic photosynthesis research and potential host for biotechnological applications. Despite recent advances genome sequencing microarray techniques applied systems biology, quantitative proteomics approaches with corresponding accuracy depth are scarce S. 6803. In this study, we developed protocol to screen changes the expression of 106 proteins representing central metabolic pathways targeted...
NAD(P)H dehydrogenases comprise type 1 (NDH-1) and 2 (NDH-2s) enzymes. Even though the NDH-1 complex is a well-characterized protein in thylakoid membrane of Synechocystis sp. PCC 6803 (hereafter Synechocystis), exact roles different NDH-2s remain poorly understood. To elucidate this question, we studied function NdbC, one three Synechocystis, by constructing deletion mutant (ƊndbC) for corresponding submitting to physiological biochemical characterization as well comprehensive proteomics...
Brassica rapa studies towards metabolic variation have largely been focused on the profiling of diversity compounds in specific crop types or regional varieties, but none aimed to identify genes with regulatory function metabolite composition. Here we followed a genetical genomics approach for six biosynthetic pathways health-related phytochemicals, i.e carotenoids, tocopherols, folates, glucosinolates, flavonoids and phenylpropanoids. Leaves from weeks-old plants doubled haploid population,...
Photomixotrophy is a metabolic state that enables photosynthetic microorganisms to simultaneously perform photosynthesis and metabolism of imported organic carbon substrates. This process complicated in cyanobacteria, since many, including Synechocystis sp. PCC 6803, conduct respiration an interlinked thylakoid membrane electron transport chain. Under photomixotrophy, the cell must therefore tightly regulate fluxes from respiratory complexes. In this study, we demonstrate, via...
One of the hallmarks marine diatom biology is their ability to cope with rapid changes in light availability due mixing water column and lens effect. We investigated how irradiance fluctuations influence relative abundance key photosynthetic proteins centric Thalassiosira pseudonana by means mass-spectrometry-based approaches for protein quantitation. Most notably, fluctuating-light conditions lead a substantial overall up-regulation light-harvesting complex as well several subunits...
Proteomes of an oxygenic photosynthetic cyanobacterium, Synechocystis sp. PCC 6803, were analyzed under photoautotrophic (low and high CO2, assigned as ATLC ATHC), photomixotrophic (MT), light-activated heterotrophic (LAH) conditions. Allocation proteome mass fraction to seven sub-proteomes differential expression individual proteins analyzed, paying particular attention photosynthesis carbon metabolism-centered affected by the quality quantity source light regime upon growth. A distinct...
New molecular information on potential therapeutic targets or tools for noninvasive diagnosis endometriosis are important patient care and treatment. However, surprisingly few efforts have described at the protein level. In this work we enumerate proteins in endometrium ovarian endometrioma by extensive comprehensive analysis of minute amounts cryosectioned tissues a three-tiered mass spectrometric approach. Quantitative comparison revealed 214 differentially expressed endometrium. These...
Abstract Background Advancing the engineering of photosynthesis-based prokaryotic cell factories is important for sustainable chemical production and requires a deep understanding interplay between bioenergetic metabolic pathways. Rearrangements in photosynthetic electron flow to increase efficient use light energy carbon fixation must be balanced with strong sink avoid photoinhibition. In cyanobacterium Synechocystis sp. PCC 6803, flavodiiron protein Flv3 functions as an alternative...
NdbA, one of the three type 2 NAD(P)H dehydrogenases (NDH-2) in Synechocystis sp. PCC 6803 (hereafter Synechocystis) was here localized to thylakoid membrane (TM), unique for NDH-2s, and investigated with respect photosynthetic cellular redox metabolism. For this purpose, a deletion mutant (ΔndbA) complementation strain overexpressing NdbA (ΔndbA::ndbA) were constructed. It is demonstrated that expressed at very low level wild-type (WT) under photoautotrophic (PA) growth whilst substantially...
Cyanobacteria are prokaryotes performing oxygenic photosynthesis, and they can be engineered to harness solar energy for production of commodity high-value chemicals by means synthetic biology. The Cu2+ -regulated petJ promoter (PpetJ ), which controls the expression endogenous cytochrome c553, used foreign products in Synechocystis 6803. We aimed disclose potential bottlenecks application PpetJ biology approaches.Quantitative label-free mass spectrometry revealed global proteome changes...
Nostoc (Anabaena) sp. PCC 7120 is a filamentous cyanobacterial species that fixes N2 to nitrogenous compounds using specialised heterocyst cells. Changes in the intracellular ratio of carbon nitrogen (C/N balance) known trigger major transcriptional reprogramming cell, including initiating differentiation vegetative cells heterocysts. Substantial analysis has been performed on during N stepdown (low high C/N), but not C (high low C/N). In current study, we shifted metabolic balance cultures...