A5020192058- Photosynthetic Processes and Mechanisms
- Marine and coastal ecosystems
- Algal biology and biofuel production
- Diatoms and Algae Research
- Microbial Fuel Cells and Bioremediation
- Advanced biosensing and bioanalysis techniques
- CRISPR and Genetic Engineering
- ATP Synthase and ATPases Research
- Protist diversity and phylogeny
- COVID-19 epidemiological studies
- Vaccine Coverage and Hesitancy
- Microbial Community Ecology and Physiology
- SARS-CoV-2 and COVID-19 Research
- Electrochemical sensors and biosensors
University of East Anglia
2017-2022
Norwich Research Park
2017-2022
University of York
2020-2022
University of Turku
2011-2016
Leipzig University
2006-2009
The thylakoid membrane of photoautotrophic organisms contains the main components photosynthetic electron transport chain. Detailed proteome maps protein complexes two marine diatoms, Thalassiosira pseudonana and Phaeodactylum tricornutum, were created by means two-dimensional blue native (BN)/SDS-PAGE coupled with mass spectrometry analysis. One novel diatom-specific photosystem I (PS I)-associated was identified. A second plastid-targeted possible PS interaction discovered to be restricted...
In this study, the enzyme activity of partially purified diadinoxanthin de‐epoxidase (DDE) from diatom Cyclotella meneghiniana was investigated at different ascorbate concentrations and pH values. comparison with spinach violaxanthin (VDE), we found a much higher affinity for co‐substrate ascorbate. The K m value DDE 5 (0.7 M ) significantly lower than that observed VDE (2.3 ). pH‐optimum low concentrations. At high concentrations, strong shift optimum towards values, significant still...
In the present study we report that in diatom Cyclotella meneghiniana diatoxanthin-dependent non-photochemical quenching of chlorophyll fluorescence (NPQ) is heterogeneous and consists three different components. (i) A transient NPQ component generates immediately upon illumination, depends on transthylakoid proton gradient as well light intensity, modulated by initial diatoxanthin content cells. It located antenna complexes C. comparable with observed vascular plants. (ii) steady-state...
CRISPR/Cas enables targeted genome editing in many different plant and algal species including the model diatom Thalassiosira pseudonana. However, efficient gene targeting by homologous recombination (HR) to date is only reported for photosynthetic organisms their haploid life-cycle phase. Here, a construct, assembled using Golden Gate cloning, enabled highly HR diploid organism. Homologous was induced T. pseudonana sequence-specific CRISPR/Cas, paired with dsDNA donor matrix, generating...
The ability to clone genes has greatly advanced cell and molecular biology research, enabling researchers generate fluorescent protein fusions for localization confirm genetic causation by mutant complementation. Most gene cloning is polymerase chain reaction (PCR)�or DNA synthesis-dependent, which can become costly technically challenging as increase in size, particularly if they contain complex regions. This been a long-standing challenge the Chlamydomonas reinhardtii research community,...
One of the hallmarks marine diatom biology is their ability to cope with rapid changes in light availability due mixing water column and lens effect. We investigated how irradiance fluctuations influence relative abundance key photosynthetic proteins centric Thalassiosira pseudonana by means mass-spectrometry-based approaches for protein quantitation. Most notably, fluctuating-light conditions lead a substantial overall up-regulation light-harvesting complex as well several subunits...
Genome editing in diatoms has recently been established for the model species Phaeodactylum tricornutum and Thalassiosira pseudonana.The present protocol, although developed T. pseudonana, can be modified to edit any diatom genome as we utilize flexible, modular Golden Gate cloning system.The main steps include how design a construct using targeting two sites, allowing precise deletion introduced into target gene.The transformation protocol is explained, are methods screening band shift...
Abstract The regulated abundance and spatial distribution of proteins determines cellular structure function. discovery green fluorescent protein (GFP) fusing it to a target determine subcellular localization revolutionized cell biology. Most studies involve introducing additional copies gene genetically fused GFP under the control constitutive promoter, resulting in expression GFP-fusion at non-native levels. Here we have developed single vector CRISPR/Cas9 guided knock-in strategy diatom...
Abstract CRISPR/Cas enables targeted genome editing in many different plant and algal species including the model diatom Thalassiosira pseudonana . However, efficient gene targeting by homologous recombination (HR) to date is only reported for photosynthetic organisms their haploid life-cycle phase there are no examples of nuclease-meditated HR any organism. Here, a construct, assembled using Golden Gate cloning, enabled highly first time diploid was induced T. means sequence specific...
Abstract The ability to clone genes has driven fundamental advances in cell and molecular biology, enabling researchers introduce precise mutations, generate fluorescent protein fusions for localization confirm genetic causation by mutant complementation. Most gene cloning is PCR or DNA synthesis dependent, which can become costly technically challenging as increase size particularly if they contain complex regions. This been a long-standing challenge the Chlamydomonas reinhardtii research...