A5084896915- Algal biology and biofuel production
- Photosynthetic Processes and Mechanisms
- Marine and coastal ecosystems
- Diatoms and Algae Research
- Microbial Community Ecology and Physiology
- Aquatic Ecosystems and Phytoplankton Dynamics
- Light effects on plants
- Protist diversity and phylogeny
- Biocrusts and Microbial Ecology
- Photoreceptor and optogenetics research
- Spectroscopy Techniques in Biomedical and Chemical Research
- Spectroscopy and Chemometric Analyses
- Silicon Carbide Semiconductor Technologies
- Plant responses to elevated CO2
- Cryospheric studies and observations
- Antioxidant Activity and Oxidative Stress
- Marine and coastal plant biology
- Water Quality Monitoring and Analysis
- Advanced DC-DC Converters
- Winter Sports Injuries and Performance
- Metabolomics and Mass Spectrometry Studies
- Plant Stress Responses and Tolerance
- Spectroscopy and Quantum Chemical Studies
- Identification and Quantification in Food
- X-ray Diffraction in Crystallography
Hochschule Bremerhaven
2019-2025
Leipzig University
2014-2024
Universitätsklinikum Würzburg
2024
Kantonsspital Graubünden
2003-2022
German Centre for Integrative Biodiversity Research
2014-2021
Saxon Institute for Applied Biotechnology
2019-2021
University of Tübingen
2021
École des Hautes Études en Santé Publique
2017
Genomics (United Kingdom)
2016
Audi (Germany)
2012-2014
According to general agreement, all photosynthetic organisms using xanthophyll cycling for photoprotection contain either the violaxanthin (Vx) cycle or diadinoxanthin (Ddx) instead. Here, we report temporal accumulation of substantial amounts pigments Vx under prolonged high-light stress in several microalgae thought possess only Ddx cycle. In diatom Phaeodactylum tricornutum, used as a model organism, these also participate cycling, and their depends on de novo synthesis carotenoids...
• The balance of energy flow from light absorption into biomass was investigated under simulated natural conditions in the diatom Phaeodactylum tricornutum and green alga Chlorella vulgaris. quantified by comparative analysis carbon accumulation new with photosynthetic electron transport rates per absorbed quantum, measured both fluorescence quenching oxygen production. difference between fluorescence- oxygen-based is defined as ‘alternative cycling’. efficiency production found to be...
To understand the fine-scale effects of changes in nutrient availability on eukaryotic soil microorganisms communities, a multiple barcoding approach was used to analyse samples from four different treatments long-term fertilization experiment. We performed PCR amplification DNA with primer pairs specifically targeting 18S rRNA genes all eukaryotes and three protist groups (Cercozoa, Chrysophyceae-Synurophyceae Kinetoplastida) as well ITS gene fungi 23S plastid photoautotrophic...
We studied the localization of diadinoxanthin cycle pigments in diatoms Cyclotella meneghiniana and Phaeodactylum tricornutum. Isolation pigment protein complexes revealed that majority high-light-synthesized diatoxanthin is associated with fucoxanthin chlorophyll (FCP) complexes. The characterization intact cells, thylakoid membranes, by absorption low-temperature fluorescence spectroscopy showed FCPs contain certain amounts protein-bound pigments, which are not significantly different...
The objective of the present study was to test hypothesis that acclimation different light intensities in diatom Phaeodactylum tricornutum is controlled by quality perception mechanisms. Therefore, semi-continuous cultures P. were illuminated with equal amounts photosynthetically absorbed radiation blue (BL), white (WL), and red (RL) combination two irradiance, low (LL) medium (ML). Under LL conditions, growth rates photosynthesis similar for all cultures. However, BL found be an state...
Abstract Cell division in photosynthetic organisms is tightly regulated by light. Although the light dependency of onset cell cycle has been well characterized various phototrophs, little known about cellular signaling cascades connecting perception to activation and progression. Here, we demonstrate that diatom-specific cyclin 2 (dsCYC2) Phaeodactylum tricornutum displays a transcriptional peak within 15 min after exposure, long before division. The product dsCYC2 binds cyclin-dependent...
A mid-infrared spectroscopic method was developed for the simultaneous and quantitative determination of total protein, carbohydrate lipid contents microalgal cells. Based on a chemometric approach, measured FTIR (Fourier transform infrared) spectra from algal cells were reconstructed by partial least square algorithm, using reference substances to determine their relative contribution overall cell spectrum. From this specific absorption, absolute macromolecular composition [pg cell(-1)] can...
Chromist algae (stramenopiles, cryptophytes, and haptophytes) are major contributors to marine primary productivity. These eukaryotes acquired their plastid via secondary endosymbiosis, whereby an early-diverging red alga was engulfed by a protist the retained its associated nuclear-encoded genes were transferred host genome. Current data suggest, however, that chromists paraphyletic; therefore, it remains unclear whether plastids trace back single endosymbiosis or, alternatively, this...
The photosynthetic antenna system of diatoms contains fucoxanthin chlorophyll a/c binding proteins (FCPs), which are membrane intrinsic showing high homology to the light harvesting complexes (LHC) higher plants. In present study, we used a mild solubilization P. tricornutum thylakoid membranes in combination with sucrose density gradient centrifugation or gelfiltration and obtained an oligomeric FCP complex (FCPo). spectroscopic characteristics pigment stoichiometries FCPo were comparable...
Aureochromes constitute a family of blue light (BL) receptors which are found exclusively in heterokont algae such as diatoms (Bacillariophyceae) and yellow-green (Xanthophyceae). Previous studies on the diatom Phaeodactylum tricornutum indicate that formation high acclimated phenotype is mediated by absorption BL aureochromes might play an important role this process. P. possesses four genes encoding aureochromes. In study we confirm nuclear localisation PtAUREO1a, 1b 2 proteins....