A5053826607- Botanical Research and Chemistry
- Analytical Chemistry and Chromatography
- Phytochemicals and Antioxidant Activities
- Chromatography in Natural Products
- Plant and fungal interactions
- Metabolomics and Mass Spectrometry Studies
- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Plant Stress Responses and Tolerance
- Plant biochemistry and biosynthesis
- Plant Gene Expression Analysis
- Natural product bioactivities and synthesis
- Phytochemistry and Biological Activities
- Genetic and Environmental Crop Studies
- Plant-Microbe Interactions and Immunity
- Plant tissue culture and regeneration
- Wheat and Barley Genetics and Pathology
- Analytical chemistry methods development
- Pesticide and Herbicide Environmental Studies
- Mycotoxins in Agriculture and Food
- Synthesis and Reactions of Organic Compounds
- Polysaccharides and Plant Cell Walls
- Legume Nitrogen Fixing Symbiosis
- Structural and Chemical Analysis of Organic and Inorganic Compounds
- Metabolism and Genetic Disorders
Institute of Bioorganic Chemistry, Polish Academy of Sciences
2012-2022
Polish Academy of Sciences
1993-2016
Universidad de Guadalajara
2010
Institute of Plant Genetics, Polish Academy of Sciences
2010
Adam Mickiewicz University in Poznań
1979-2002
Cyclopharma (France)
1994
Harvard University
1990
Plant responses to abiotic stresses are very complex phenomena with individual characteristics for various species. Abiotic (e.g. drought, salinity, flooding, cold, heat, UV radiation, heavy metals, etc.) strongly affect plant growth and development. It is estimated that they the cause of more than 50 % crop yield losses. known activate a multigene response resulting in changes proteins primary secondary metabolite accumulation. Therefore, proteomic metabolomic approaches becoming important...
In this study, proteomic and metabolomic changes in leaves roots of two barley (Hordeum vulgare L.) genotypes, with contrasting drought tolerance, subjected to water deficit were investigated. Our two-dimensional electrophoresis (2D-PAGE) combined matrix-assisted laser desorption time flight mass spectrometry (MALDI-TOF MALDI-TOF/TOF) analyses revealed 121 drought-responsive proteins 182 both genotypes. Many the identified associated processes that are typically severely affected during...
Abstract Flavonoid conjugates constitute several classes of plant phenolic secondary metabolites including many isomeric compounds differing in the hydroxylation pattern and substitution their rings with different groups such as alkyls, acyls or sugars. These occur tissues mainly glycosides cases it is necessary to have reliable detailed information concerning structure these natural products. Our results were obtained using leaf extracts Arabidopsis thaliana Lupinus angustifolius which...
• In a phenotypic screen of plants constitutively overexpressing DOF (DNA-binding-with-one-finger) transcription factors under the control Cauliflower mosaic virus 35S promoter, AtDOF4;2 was identified as gene inducing bushy plant phenotype and potentially being involved in regulation phenylpropanoid metabolism Arabidopsis. Further molecular biochemical characterization performed parallel using transgenic with enhanced reduced expression. The expression pattern determined by quantitative...
The aim of this study was to present integrated mass spectrometric methods for the structural characterization and identification flavonoid glycoconjugates. During liquid chromatography/mass spectrometry analyses, TriVersa NanoMate chip‐based system with nanoelectrospray ionization fraction collection combined a quadrupole time‐of‐flight spectrometer. In extract samples prepared from green leaves wheat plantlets, 41 derivatives were recognized. Part target natural products had full structure...
Abstract Background Mass spectrometric analysis of the blood proteome is an emerging method clinical proteomics. The approach exploiting multi-protein/peptide sets (fingerprints) detected by mass spectrometry that reflect overall features a specimen's proteome, termed pattern analysis, have been already shown in several studies to applicability cancer diagnostics. We aimed identify serum patterns specific for early stage breast patients using MALDI-ToF spectrometry. Methods Blood samples...
Plant interactions with environmental factors cause changes in the metabolism and regulation of biochemical physiological processes. defense against pathogenic microorganisms depends on an innate immunity system that is activated as a result infection. There are two mechanisms triggering this system: basal perception microbe-associated molecular patterns through pattern recognition receptors situated cell surface effector-triggered (ETI). An induced biosynthesis bioactive secondary...
Hairy roots and suspension cell cultures are commonly used in deciphering different problems related to the biochemistry physiology of plant secondary metabolites. Here, we address about issue possible differences profiles flavonoid compounds their glycoconjugates derived from various materials grown a standard culture media. We compared flavonoids isolated seedling roots, hairy root model legume plant, Medicago truncatula. The analyses were conducted with isolates as well LC/MS target...
We examined changes in profiles of isoflavonoids roots lupine (Lupinus luteus L. cv. Juno) seedlings response to treatment with two heavy metals: cadmium (at 10 mg/l) and lead 150 mg/l). Overall, 21 flavonoid conjugates were identified root extracts, some them up six positional isomers. The total amount all increased by about 15 % cadmium-treated plants 46 lead-treated ones. Heavy metals markedly the content compounds: 2'-hydroxygenistein glucoside 7-O-glucoside malonylated. Possible...
Drought is a major abiotic stress that negatively influences crop yield. Breeding strategies for improved drought resistance require an knowledge of plant responses. We therefore applied to barley recombinant inbred lines and their parental genotypes shortly before tillering. A large-scale proteomic analysis leaf root tissue revealed proteins respond in genotype-specific manner. Of these, Rubisco activase chloroplast, luminal binding protein endoplasmic reticulum, phosphoglycerate mutase,...
Extracts from plantlets of different species the genus Drosera, grown as in vitro cultures, were evaluated for level phenolic secondary metabolites group naphthoquinones and flavonols. The profiles natural products extracts obtained monitored by HPLC with UV detection at 260 330 nm. On basis data obtained, Drosera binata, highest amount plumbagin, was selected further studies. most effective method extraction quinones established composition tissues determined. For identification compounds,...
Abstract Introduction: Glycosylation at different hydroxyl groups of flavonoids and acylation sugar moieties are ubiquitous modifications observed in plants. These give rise to simultaneous presence numerous isomeric isobaric compounds tissues extracts thereof. Objective: To develop UPLC‐MS method capable for resolution malonylated glycoconjugates recognition structural differences. Methodology: Flavonoid were extracted from leaves blue lupin ( Lupinus angustifolius L.) plants with 80%...
Flavonoids are important plant compounds occurring in tissues mostly the form of glycoconjugates. Most frequently sugar moiety is comprised mono- or oligosaccharides consisting common sugars like glucose, rhamnose galactose. In some species glycosidic contains glucuronic acid and may be acylated by phenylpropenoic acids.Flavonoid glyconjugates were extracted from leaves Medicago truncatula ecotype R108 submitted to analysis using high-performance liquid chromatography combined with...