A5086083270- Plant tissue culture and regeneration
- Phytoestrogen effects and research
- Potato Plant Research
- 14-3-3 protein interactions
- Polysaccharides and Plant Cell Walls
- Microbial Metabolites in Food Biotechnology
- Transgenic Plants and Applications
- Plant Genetic and Mutation Studies
- Plant-Microbe Interactions and Immunity
- Plant Molecular Biology Research
- Natural Fiber Reinforced Composites
- Phytochemicals and Antioxidant Activities
- biodegradable polymer synthesis and properties
- GABA and Rice Research
- Plant Gene Expression Analysis
- Lipid metabolism and biosynthesis
- Food composition and properties
- Wound Healing and Treatments
- DNA and Nucleic Acid Chemistry
- Seed and Plant Biochemistry
- Natural Antidiabetic Agents Studies
- Chromosomal and Genetic Variations
- Electrospun Nanofibers in Biomedical Applications
- Plant Pathogens and Resistance
- Animal Genetics and Reproduction
University of Wrocław
2015-2024
Wroclaw University of Environmental and Life Sciences
2015-2023
Wrocław University of Science and Technology
2023
AGH University of Krakow
2023
Institute of Bast Fiber Crops
2017
Wroclaw Research Centre EIT+ (Poland)
2014
University of Szczecin
2002-2007
Institute of Soil Science and Plant Cultivation
2005
Institute of Animal Reproduction and Food Research
2005
Polish Academy of Sciences
2005
Summary Glucosinolates are a group of secondary metabolites that function as defense substances against herbivores and micro‐organisms in the plant order Capparales . Indole glucosinolates (IGS), derivatives tryptophan, may also influence growth development. In Arabidopsis thaliana , indole‐3‐acetaldoxime (IAOx) produced from tryptophan by activity two cytochrome P450 enzymes, CYP79B2 CYP79B3, serves precursor for IGS biosynthesis but is an intermediate biosynthetic pathway indole‐3‐acetic...
The main goal of this study was to generate potato tubers with increased levels flavonoids and thus modified antioxidant capacities. To accomplish this, the vector carrying multigene construct prepared several transgenic plants were generated, all overexpressing key biosynthesis pathway enzymes. single-gene overexpression or simultaneous expression genes encoding chalcone synthase (CHS), isomerase (CHI), dihydroflavonol reductase (DFR) resulted in a significant increase measured phenolic...
The principal goal of this paper was to investigate the significance anthocyanin 5-O-glucosyltransferase (5-UGT) for potato tuber metabolism. ectopic expression a 5-UGT cDNA in improved plant's defense against pathogen infection. resistance transgenic lines Erwinia carotovora subsp. about 2-fold higher than nontransformed plants. In most cases accompanied by significant increase yield. To molecular basis resistance, metabolic profiling plant performed. extracts, 3,5-O-substituted level...
Flavonoids are a group of secondary plant metabolites important for growth and development. They show also protective effect against colon breast cancer, diabetes, hypercholesterolemic atherosclerosis, lupus nephritis, immune inflammatory reactions. Thus, overproduction these compounds in flax by genetic engineering method might potentiate biotechnological application products. Flax plants third generation overexpressing key genes flavonoid pathway cultivated field were used as material...
The aim of this study was to engineer a flax with increased resistance pathogens. approach based on the recent analysis Solanum sogarandinum-derived glycosyltransferase (UGT) protein, designated SsGT1 (previously called 5UGT). On basis enzyme studies, recombinant is 7-O-glycosyltransferase, natural substrates which include both anthocyanidins and flavonols such as kaempferol quercetin. Because flavonoids act antioxidants glycosylation increases stability flavonoids, it has been suggested...
Flax crop yield is limited by various environmental stress factors, but the largest losses worldwide are caused Fusarium infection. Polyamines one of many plant metabolites possibly involved in response to However, flax plants polyamine composition, genes synthesis, and particular their regulation, were previously unknown. The aim this study was investigate synthesis pathway its involvement pathogen It well established that polyamines essential for growth development both fungi, role...
Flaxseed oil is characterized by high content of essential polyunsaturated fatty acids (PUFA) promoted as a human dietary supplement protecting against atherosclerosis. The disadvantage the PUFA in flax susceptibility to oxidation, which can result carcinogenic compound formation. Linola cultivar linoleic acid comparison traditional cultivars rich linolenic acid. changes proportions increase oxidative stability and broaden its use an edible for cooking. However one investigated transgenic...
Flax straw of flax varieties that are grown for oil production is a by product which represents considerable biomass source. Therefore, its potential application human use high interest. Our research has revealed rich in flavonoid C-glucosides, including vitexin, orientin, and isoorientin. The objective this study was to evaluate the cytotoxicity possible proapoptotic effect derived C-glucosides flavonoids breast adenocarcinoma cell line (MCF-7). effects on proliferation MCF-7 cells were...
Upregulation of the terpenoid pathway and increased ABA content in flax upon Fusarium infection leads to activation early plant's response (PR genes, cell wall remodeling, redox status). Plants have developed a number defense strategies against adverse effects fungi such as oxysporum. One is production antioxidant secondary metabolites, which fall into two main groups: phenylpropanoids terpenoids. While functions biosynthesis been extensively studied, very little known about genes...
The principal goal of this paper was to generate flax (Linum usitatissimum L.) plants with increased antioxidant properties. To accomplish a vector containing multigene construct prepared, and transgenic overexpressing essential flavonoid biosynthesis pathway enzymes were generated analyzed. simultaneous expression genes encoding chalcone synthase (CHS), isomerase (CHI), dihydroflavonol reductase (DFR) resulted in significant increase capacity. investigate the determinants higher properties...
A new flax dressing product was developed based on three components (fibers, oil emulsion, and seedcake extract) from genetically engineered plants that were obtained by plant transformation using genes controlling the synthesis of antioxidative compounds phenylpropanoid pathway. Simultaneous explant with coding for chalcone synthase, isomerase, dihydroflavonol reductase resulted in an accumulation phenolic acids fibers, polyunsaturated fatty oil, lignans seedcake. The transgenic contained a...