A5066169752- Legume Nitrogen Fixing Symbiosis
- Plant nutrient uptake and metabolism
- Drug Transport and Resistance Mechanisms
- Plant-Microbe Interactions and Immunity
- Plant Reproductive Biology
- Plant tissue culture and regeneration
- Plant Parasitism and Resistance
- Plant Molecular Biology Research
- Agriculture, Plant Science, Crop Management
- RNA and protein synthesis mechanisms
- Cholesterol and Lipid Metabolism
- Plant Stress Responses and Tolerance
- Genetic and Environmental Crop Studies
- Plant Gene Expression Analysis
- Photosynthetic Processes and Mechanisms
- Plant biochemistry and biosynthesis
- Phytoestrogen effects and research
- Fungal and yeast genetics research
- Energy Load and Power Forecasting
- Agronomic Practices and Intercropping Systems
- Aluminum toxicity and tolerance in plants and animals
- Sirtuins and Resveratrol in Medicine
- Toxin Mechanisms and Immunotoxins
- Botanical Research and Chemistry
- Microbial Metabolites in Food Biotechnology
Institute of Bioorganic Chemistry, Polish Academy of Sciences
2015-2025
University of Life Sciences in Poznań
2015-2024
University of Wrocław
1998-2023
University of Fribourg
2008
University of Zurich
2003-2008
University of Geneva
2008
University of Bern
2008
Colorado State University
2007
UCLouvain
2001-2007
ATP binding cassette (ABC) transporters, which are found in all species, known mainly for their ability to confer drug resistance. To date, most of the ABC transporters characterized plants have been localized vacuolar membrane and considered be involved intracellular sequestration cytotoxins. Working on assumption that certain might defense metabolite secretion expression regulated by concentration these metabolites, we treated a Nicotiana plumbaginifolia cell culture with sclareolide,...
Cells are separated from their external environment by a membrane barrier, which ensures that certain ions, metabolic intermediates, and macromolecules, such as proteins, remain within the cell. However, life is not possible without exchange of material information with
Abstract Following recent indirect evidence suggesting a role for ATP-binding cassette (ABC) transporters in root exudation of phytochemicals, we identified 25 ABC transporter genes highly expressed the cells most likely to be involved secretion processes. Of these genes, also selected six full-length and half-size in-depth molecular biochemical analyses. We compared exuded phytochemical profiles seven mutants those wild type. There were three nonpolar phytochemicals missing various type...
Phenylpropanoids fulfill numerous physiological functions, essential for plant growth and development, as well plant-environment interactions. Over the last few decades, many studies have shown that exquisite regulatory mechanisms at multiple levels control phenylpropanoid metabolic pathway. Deciphering this pathway not only provides a greater, basic understanding of specialized metabolism, but also enhances our ability to rationally design pathways future applications. Despite...
Full-sized ATP-binding cassette (ABC) transporters of the G subfamily (ABCG) are considered to be essential components plant immune system. These proteins have been proposed implicated in active transmembrane transport various secondary metabolites. Despite importance ABCG-based for plant–microbe interactions, these still poorly recognized legumes. The experiments described here demonstrated that level Medicago truncatula ABCG10 (MtABCG10) mRNA was elevated following application fungal...
Strigolactones (SLs) are plant-derived signaling molecules that stimulate the hyphal branching of arbuscular mycorrhizal fungi (AMF), and consequently promote symbiotic interaction between fungus plant. Currently, our knowledge on molecular mechanism SL transport is restricted to Solanaceae family. In family, translocation towards rhizosphere occurs through exodermis via hypodermal passage cells involves a member G subfamily, ATP-binding cassette (ABC) membrane transporters. Most Fabaceae...
Abstract The analysis of gene expression in Arabidopsis (Arabidopsis thaliana) using cDNA microarrays and reverse transcription-polymerase chain reaction showed that AtOSA1 (A. thaliana oxidative stress-related Abc1-like protein) transcript levels are influenced by Cd2+ treatment. comparison protein sequences revealed belongs to the family Abc1 proteins. Up now, proteins have been identified prokaryotes mitochondria eukaryotes. is first member this be localized chloroplasts. However, despite...
Grape (Vitis vinifera) accumulates various polyphenolic compounds, which protect against environmental stresses, including ultraviolet-C (UV-C) light and pathogens. In this study, we looked at the transcriptome metabolome in grape berry skin after UV-C irradiation, demonstrated effectiveness of omics approaches to clarify important traits grape. We performed analysis using a genome-wide microarray, revealed 238 genes up-regulated more than 5-fold by light. Enrichment Gene Ontology terms...
The ABCG10 protein of the model legume Medicago truncatula is required for efficient de novo production phenylpropanoid-derived phytoalexin medicarpin. Silencing expression MtABCG10 results, inter alia, in a lower accumulation medicarpin and its precursors. In this study, we demonstrate that impairment biosynthesis can be partially averted by exogenous application 4-coumarate, an early precursor core phenylpropanoid pathway, deoxyisoflavonoid formononetin. Experiments conducted using HPLC/MS...
Abscisic acid (ABA) integrates internal and external signals to coordinate plant development, growth architecture. It plays a central role in stomatal closure, prevents germination of freshly produced seeds non-dormant under unfavorable circumstances. Here, we describe Medicago truncatula ATP-binding cassette (ABC) transporter, MtABCG20, as an ABA exporter present roots germinating seeds. In seeds, MtABCG20 was found the hypocotyl-radicle transition zone embryonic axis. Seeds mtabcg20 plants...
The plant hormone auxin must be transported throughout plants in a cell-to-cell manner to affect its various physiological functions. ABCB transporters are critical for this polar distribution, but the regulatory mechanisms controlling their function is not fully understood. transport activity of ABCB1 was suggested regulated by physical interaction with FKBP42/Twisted Dwarf1 (TWD1), peptidylprolyl cis-trans isomerase (PPIase), all attempts demonstrate such PPIase TWD1 have failed so far. By...
ABC transporters are ubiquitous in all organisms and constitute one of the largest protein families. The substantial expansion this family plants coincided with emergence fundamental novelties that facilitated successful adaptation to a sessile lifestyle on land. It also resulted selectivity multispecificity toward endogenous molecules observed for certain transporters. Understanding molecular determinants behind intriguing feature remains an ongoing challenge functional characterization...
Plant pathogenesis‐related (PR) proteins of class 10 are the only group among 17 PR protein families that intracellular and cytosolic. Sequence conservation wide distribution PR‐10 throughout plant kingdom an indication indispensable function in plants, but their true biological role remains obscure. Crystal solution structures for several homologues have shown a similar overall fold with vast internal cavity which, together structural similarities to steroidogenic acute regulatory...
Hairy roots and suspension cell cultures are commonly used in deciphering different problems related to the biochemistry physiology of plant secondary metabolites. Here, we address about issue possible differences profiles flavonoid compounds their glycoconjugates derived from various materials grown a standard culture media. We compared flavonoids isolated seedling roots, hairy root model legume plant, Medicago truncatula. The analyses were conducted with isolates as well LC/MS target...
Flavonoids are important plant compounds occurring in tissues mostly the form of glycoconjugates. Most frequently sugar moiety is comprised mono- or oligosaccharides consisting common sugars like glucose, rhamnose galactose. In some species glycosidic contains glucuronic acid and may be acylated by phenylpropenoic acids.Flavonoid glyconjugates were extracted from leaves Medicago truncatula ecotype R108 submitted to analysis using high-performance liquid chromatography combined with...
ABCG46 of the legume Medicago truncatula is an ABC-type transporter responsible for highly selective translocation phenylpropanoids, 4-coumarate, and liquiritigenin, over plasma membrane. To investigate molecular determinants observed substrate selectivity, we applied a combination phylogenetic biochemical analyses, AlphaFold2 structure prediction, dynamics simulations, mutagenesis. We discovered unusually narrow transient access path to central cavity MtABCG46 that constitutes initial...
Full-size ATP-binding cassette (ABC) transporters belonging to the ABCG subfamily are unique for plants and fungi. There is growing evidence that certain of these proteins play a role in plant defense or signaling systems. As yet, complete set full-size protein genes has been inventoried classified only two plants: Arabidopsis thaliana Oryza sativa. Recently, domain-based clustering analysis predicted presence at least 12 encoding such Lotus japonicus genome. Here, we identify classify 19...
Nitrogen (N) as well Phosphorus (P) are key nutrients determining crop productivity. Legumes have developed strategies to overcome nutrient limitation by, for example, forming a symbiotic relationship with N-fixing rhizobia and the release of P-mobilizing exudates thus able grow without supply N or P fertilizers. The legume-rhizobial symbiosis starts root isoflavonoids that act signaling molecules perceived by compatible bacteria. Subsequently, bacteria nod factors, which induce cascades...