A5064092638- Genomics and Phylogenetic Studies
- Bacterial Genetics and Biotechnology
- Enzyme Structure and Function
- Photosynthetic Processes and Mechanisms
- Protist diversity and phylogeny
- Microbial Community Ecology and Physiology
- Protein Structure and Dynamics
- Algal biology and biofuel production
- Monoclonal and Polyclonal Antibodies Research
- Microtubule and mitosis dynamics
- Peptidase Inhibition and Analysis
- Fibroblast Growth Factor Research
- Cancer-related Molecular Pathways
- Biochemical and Molecular Research
- Protein Kinase Regulation and GTPase Signaling
- Bacteriophages and microbial interactions
- T-cell and B-cell Immunology
- RNA and protein synthesis mechanisms
- Ion channel regulation and function
- Biofuel production and bioconversion
- Receptor Mechanisms and Signaling
- Biocrusts and Microbial Ecology
- Glycosylation and Glycoproteins Research
- Bone health and treatments
- Enzyme Production and Characterization
Nanospectra Biosciences (United States)
2024
State Street (United States)
2024
Janssen (United States)
2014-2020
Johnson & Johnson (United States)
2013
ActiveSite Pharmaceuticals (United States)
2009
Iowa State University
2005
California Institute of Technology
1993-2002
Diversitech (United States)
1998-2002
University of California, Santa Barbara
1997
University of Oregon
1996
Salmonella typhimurium is a facultative intracellular pathogen capable of surviving within phagocytic cells the reticuloendothelial system. To identify genes important for survival, 9516 independent Tn10 insertional mutations were isolated in virulent strain S. typhimurium. By using an vitro assay survival macrophages, 83 mutants have been identified that diminished capacity (designated MS or macrophage mutants). All are less than parent vivo, demonstrating that, Salmonella, essential...
Methanogenesis, the biological production of methane, plays a pivotal role in global carbon cycle and contributes significantly to warming. The majority methane nature is derived from acetate. Here we report complete genome sequence an acetate-utilizing methanogen, Methanosarcina acetivorans C2A. Methanosarcineae are most metabolically diverse methanogens, thrive broad range environments, unique among Archaea forming complex multicellular structures. This diversity reflected M. . At...
Abstract CD47, a broadly expressed cell surface protein, inhibits phagocytosis via interaction with phagocyte-expressed SIRPα. A variety of hematological malignancies demonstrate elevated CD47 expression, suggesting that may mediate immune escape. We discovered three unique CD47-SIRPα blocking anti-CD47 monoclonal antibodies (mAbs) low nano-molar affinity to human and cynomolgus monkey no hemagglutination platelet aggregation activity. To characterize the anti-cancer activity elicited by...
ABSTRACT Planktonic crenarchaeotes are present in high abundance Antarctic winter surface waters, and they also make up a large proportion of total cell numbers throughout deep ocean waters. To better characterize these uncultivated marine crenarchaeotes, we analyzed genome fragments from individuals recovered single picoplankton population compared them to those representative obtained deeper waters the temperate North Pacific. Sequencing analysis entire DNA insert one archaeon (fosmid...
ABSTRACT Molecular phylogenetic surveys have recently revealed an ecologically widespread crenarchaeal group that inhabits cold and temperate terrestrial marine environments. To date these organisms resisted isolation in pure culture, so their phenotypic genotypic characteristics remain largely unknown. characterize archaea, to extend methodological approaches for characterizing uncultivated microorganisms, we initiated genomic analyses of the nonthermophilic crenarchaeote Cenarchaeum...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTResponse Regulators CheB and CheY Exhibit Competitive Binding to the Kinase CheAJiayin Li, Ronald V. Swanson, Melvin I. Simon, Robert M. WeisCite this: Biochemistry 1995, 34, 45, 14626–14636Publication Date (Print):November 1, 1995Publication History Published online1 May 2002Published inissue 1 November 1995https://pubs.acs.org/doi/10.1021/bi00045a003https://doi.org/10.1021/bi00045a003research-articleACS PublicationsRequest reuse...
Two distinct exo-acting, β-specific glycosyl hydrolases were purified to homogeneity from crude cell extracts of the hyperthermophilic archaeon Pyrococcus furiosus: a β-glucosidase, corresponding one previously by Kengen et al. (Kengen, S. W. M., Luesink, E. J., Stams, A. J. and Zehnder, B. (1993) Eur. Biochem. 213, 305-312), β-mannosidase. The β-mannosidase β-glucosidase genes isolated genomic library expression screening. nucleotide sequences predicted polypeptides with 510 472 amino acids...
Summary CheA is a dimeric autophosphorylating protein kinase that plays critical role in the signal transduction network controlling chemotaxis In Escherichia coli . The autophosphorylation reaction was analysed using mutant proteins defective and regulatory functions. Proteins which site of mutated (CheA48HQ) or missing (CheA s ) were found to phosphorylate kinase‐defective mutant, CheA470GK. kinetics this support hypothesis result trans‐phosphorylation within dimer. carboxy‐terminal...
The histidine protein kinase CheA is a central component of the Escherichia coli chemotaxis system. autophosphorylation activity controlled by membrane-bound chemoreceptors and CheW coupling protein. phosphorylates CheY CheB proteins which respectively control direction flagellar rotation level receptor adaptation, thereby regulating cells' chemotactic response. Genes encoding three polypeptide fragments were constructed expressed in order to better define functional organization wild-type...
Cenarchaeum symbiosum, an archaeon which lives in specific association with a marine sponge, belongs to recently recognized nonthermophilic crenarchaeotal group that inhabits diverse cold and temperate environments. Nonthermophilic crenarchaeotes have not yet been obtained laboratory culture, so their phenotypic characteristics inferred solely from ecological distribution. Here we report on the first protein be characterized one of these organisms. The DNA polymerase gene C. symbiosum was...
Abstract Mutants of the cyanobacterium Synechococcus sp. PCC 7002 constructed by insertional inactivation either cpcE or cpcF gene produce low levels spectroscopically detectable phycocyanin. The majority phycocyanin produced in these strains appears to lack alpha subunit phycocyanobilin (PCB) chromophore (Zhou, J., Gasparich, G. E., Stirewalt, V. L., de Lorimier, R., and Bryant, D. A. (1992) J. Biol. Chem. 267, 16138-16145). Purification mutants revealed two fractions each with an aberrant...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNMR studies of the phosphotransfer domain histidine kinase CheA from Escherichia coli: assignments, secondary structure, general fold, and backbone dynamicsHongjun Zhou, David F. Lowry, Ronald V. Swanson, Melvin I. Simon, Frederick W. DahlquistCite this: Biochemistry 1995, 34, 42, 13858–13870Publication Date (Print):October 1, 1995Publication History Published online1 May 2002Published inissue 1 October 1995https://doi.org/10.1021/bi00042a018RIGHTS...
583 Background: Hepatocellular carcinoma (HCC) is an aggressive malignancy that accounts for approximately 80-90% of all primary liver cancers and projected to become the third leading cause cancer-related mortality by 2030. Previous work demonstrated human Fibroblast Growth Factor 19 (FGF19) was overexpressed in 20-30% HCC, thereby exerting oncogenic effect via signaling through its cognate receptors FGFR4, FGFR3, co-receptor Klotho b (KLB). Multiple selective covalent FGFR4 inhibitors have...
Achondroplasia (ACH) and hypochondroplasia (HCH), the two most common types of dwarfism, are each caused by FGFR3 gain-of-function mutations that result in increased signaling, disrupting chondrogenesis osteogenesis resulting disproportionately shortened long bones. In this study, TYRA-300, a potent selective inhibitor, was evaluated three genetic contexts: wild-type mice, Fgfr3Y367C/+ mouse model ACH, Fgfr3N534K/+ HCH. model, TYRA-300 treatment naso-anal length, tibia femur length....
The glycosyl hydrolases are an important group of enzymes that responsible for cleaving a range biologically significant carbohydrate compounds. Structural information on these has provided useful their molecular basis the functional variations, while characterization structural features account high thermostability proteins is great scientific and biotechnological interest. To ends we have determined crystal structure beta-glycosidase from hyperthermophilic archeon Thermosphaera aggregans....
Abstract The crystal structure of CheY protein from Thermotoga maritime has been determined in four forms with and without Mg++ bound, at up to 1.9 resolution. Structural comparisons Escherichia coli shows substantial similarity their folds, some concerted changes propagating away the active site that suggest how phosphorylated CheY, a signal transduction bacterial chemotaxis, is recognized by its targets. A highly conserved segment (the “‐γ‐turn loop,” residues 55‐61), previously suggested...