A5080287144- Muscle Physiology and Disorders
- Adipose Tissue and Metabolism
- Amyotrophic Lateral Sclerosis Research
- Neurogenetic and Muscular Disorders Research
- Diet and metabolism studies
- Mitochondrial Function and Pathology
- Muscle metabolism and nutrition
- Single-cell and spatial transcriptomics
- Virus-based gene therapy research
- Gene expression and cancer classification
- 3D Printing in Biomedical Research
- Genetics and Physical Performance
- Adipokines, Inflammation, and Metabolic Diseases
- Diagnosis and Treatment of Venous Diseases
- Genetics, Aging, and Longevity in Model Organisms
- Gene Regulatory Network Analysis
- Autophagy in Disease and Therapy
- Cholesterol and Lipid Metabolism
- Nuclear Structure and Function
- Knee injuries and reconstruction techniques
- Lysosomal Storage Disorders Research
- Lipid metabolism and biosynthesis
- Endoplasmic Reticulum Stress and Disease
- Neuroinflammation and Neurodegeneration Mechanisms
- Edible Oils Quality and Analysis
Fondazione Santa Lucia
2024-2025
Istituto Superiore di Sanità
2025
Policlinico Tor Vergata
2022-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2024-2025
Saint Petersburg State Pediatric Medical University
2025
University of Rome Tor Vergata
2016-2022
University of Milan
2005
Ospedale Regina Margherita
1996
Vita-Salute San Raffaele University
1993
Patients with ALS show, in addition to the loss of motor neurons spinal cord, brainstem, and cerebral cortex, an abnormal depletion energy stores alongside hypermetabolism. In this study, we show that bioenergetic defects muscle remodeling occur skeletal SOD1G93A mouse model mice prior disease onset before activation denervation markers, respectively. These changes physiology were followed by increase expenditure unrelated physical activity. Finally, chronic treatment Ranolazine,...
In Duchenne muscular dystrophy (DMD), the absence of dystrophin protein causes a variety poorly understood secondary effects. Notably, muscle fibers dystrophic individuals are characterized by mitochondrial dysfunctions, as revealed reduced ATP production rate and defective oxidative phosphorylation. Here, we show that in mouse model DMD ( mdx ), fibro/adipogenic progenitors (FAPs) dysfunctional metabolism which correlates with increased adipogenic potential. Using high-sensitivity mass...
Background: Amyotrophic lateral sclerosis (ALS) is a fatal neuromuscular disease characterized by the degeneration of motor neurons that leads to muscle wasting and atrophy. Epidemiological experimental evidence suggests causal relationship between ALS physical activity (PA). However, impact PA on neuron loss sarcopenia still debated, probably because heterogeneity intensities proposed exercises. With this study, we aimed clarify effect intense endurance exercise onset progression in...
Today, among chronic immune-mediated systemic diseases, celiac disease, or gluten enteropathy, occupies one of the leading places in terms degree study and development gastroenterology therapy. Despite these facts, disease is interest from point view scientific research, as a direct correlation between genetically determined component has been proven, research direction which promises doctors to find preventive points impact combination with knowledge immunobiology genetics. The purpose this...
The regeneration of the muscle tissue relies on capacity satellite stem cell (SC) population to exit quiescence, divide asymmetrically, proliferate, and differentiate. In age-related atrophy (sarcopenia) several dystrophies, cannot compensate for loss tissue. These disorders are associated with depletion pool or functionality. Recently, establishment maintenance quiescence in cells have been linked their metabolic state. this work, we aimed modulate metabolism order preserve pool. We made...
Abstract The term micro-heterogeneity refers to non-genetic cell variability observed in a bell-shaped distribution of the expression trait within population. contribution physiology and pathology remains largely uncharacterised. To address such an issue, we investigated impact heterogeneity skeletal muscle fibro/adipogenic progenitors (FAPs) isolated from animal model Duchenne muscular dystrophy (DMD), mdx mouse. FAPs play essential role homoeostasis. However, pathological conditions or...
Amyotrophic Lateral Sclerosis (ALS) is considered the prototype of motor neuron disease, characterized by loss and muscle waste. A well-established pathogenic hallmark ALS mitochondrial failure, leading to bioenergetic deficits. So far, pharmacological interventions for disease have proven ineffective. Trimetazidine (TMZ) described as a metabolic modulator acting on different cellular pathways. Its efficacy in enhancing muscular cardiovascular performance has been widely described, although...
Abstract Fibro/Adipogenic Progenitors (FAPs) define a stem cell population playing pro-regenerative role after muscle damage. When removed from their natural niche, FAPs readily differentiate into adipocytes or fibroblasts. This digressive differentiation potential, which is kept under tight control in the healthy contributes to fat and scar infiltrations degenerative myopathies, such as Duchenne Muscular Dystrophy (DMD). Controlling FAP by means of small molecules may contribute delay...
The interstitial space surrounding the skeletal muscle fibers is populated by a variety of mononuclear cell types. Upon acute or chronic insult, these populations become activated and initiate finely-orchestrated crosstalk that promotes myofiber repair regeneration. Mass cytometry powerful highly multiplexed technique for profiling single-cells. Herein, it was used to dissect dynamics in physiological pathological conditions. Here, we characterized an antibody panel could be identify most...
Accumulating evidence suggests that dysfunctional adipose tissue (AT) plays a major role in the risk of developing multiple sclerosis (MS), most common immune-mediated and demyelinating disease central nervous system. However, contribution to etiology progression MS is still obscure. This study aimed at deciphering responses AT experimental autoimmune encephalomyelitis (EAE), best characterized animal model MS.We observed significant loss EAE mice onset disease, with infiltration M1-like...
The metabolic perturbation caused by calorie restriction enhances muscle repair playing a critical role in regulating satellite cell availability and activity the muscles of young old mice. To clarify underlying mechanisms we asked whether myoblast replication differentiation are affected metformin, restriction-mimicking drug. C2C12, mouse line, readily differentiate vitro fuse to form myotubes. However, when incubated with C2C12 slow their do not differentiate. Interestingly, lower doses...
Low-protein/high-carbohydrate (LPHC) diet has been suggested to promote metabolic health and longevity in adult humans animal models. However, the complex molecular underpinnings of how LPHC leads benefits remain elusive. Through a multi-layered approach, here we observed that promotes an energy-dissipating response consisting parallel recruitment canonical non-canonical (muscular) thermogenic systems subcutaneous white adipose tissue (sWAT). In particular, measured Ucp1 induction...
Abstract Heterotopic ossification (HO) is a pathological condition characterized by the deposition of mineralized tissue in ectopic locations such as skeletal muscle. The precise cellular origin and molecular mechanisms underlying HO are still debated. In our study we focus on differentiation mesoangioblasts (MABs), population multipotent muscle precursors. High-content screening for small molecules that perturb MAB decisions identified Idoxuridine (IdU), an antiviral radiotherapy adjuvant,...
Muscle regeneration is a complex process governed by the interplay between several muscle-resident mononuclear cell populations. Following acute or chronic damage these populations are activated, communicate via cell-cell interactions and/or paracrine signals, influencing fate decisions activation repression of internal signaling cascades. These highly dynamic processes, occurring with distinct temporal and spatial kinetics. The main challenge toward system level description muscle...
Abstract Background Peripheral immune cells critically contribute to the clinical‐pathological progression of neurodegenerative diseases and also represent a reliable frame for translational applications. However, data on progressive supranuclear palsy (PSP) are almost scarce in this regard. Objective Our goal is provide broad biological characterization peripheral selected PSP cohort. Methods Seventy‐one patients scored Rating Scale (PSPRS), 59 controls were enrolled. The blood cell count...
Abstract Recent studies demonstrated reduced blood lysosomal acid lipase (LAL) activity in patients with nonalcoholic fatty liver disease (NAFLD). We aimed to verify hepatic LAL protein content and vitro vivo models of fat overload NAFLD patients. were firstly evaluated Huh7 cells exposed high-glucose/high-lipid (HGHL) medium the C57BL/6 mice fed high-fat diet (HFD) for 4 8 months. was also by immunohistochemistry biopsies from 87 10 controls, correlated histology. treated HGHL showed a...