A5014588475- Amyotrophic Lateral Sclerosis Research
- Neuroscience and Neuropharmacology Research
- Parkinson's Disease Mechanisms and Treatments
- Neurogenetic and Muscular Disorders Research
- Sulfur Compounds in Biology
- Neuroinflammation and Neurodegeneration Mechanisms
- Ion channel regulation and function
- Cholinesterase and Neurodegenerative Diseases
- Alzheimer's disease research and treatments
- Receptor Mechanisms and Signaling
- Stress Responses and Cortisol
- Autophagy in Disease and Therapy
- Neurogenesis and neuroplasticity mechanisms
- Memory and Neural Mechanisms
- Amino Acid Enzymes and Metabolism
- Genetics and Neurodevelopmental Disorders
- Neurotransmitter Receptor Influence on Behavior
- Nerve injury and regeneration
- Neuroscience of respiration and sleep
- Neurological disorders and treatments
- Mast cells and histamine
- Neurological diseases and metabolism
- Nicotinic Acetylcholine Receptors Study
- RNA and protein synthesis mechanisms
- Neuropeptides and Animal Physiology
Fondazione Santa Lucia
2015-2024
Istituti di Ricovero e Cura a Carattere Scientifico
2002-2024
Institute of Neurobiology and Molecular Medicine
2015-2018
University of Illinois Chicago
1996-1999
Illinois College
1997-1999
University of Illinois Urbana-Champaign
1997
Nathan Kline Institute for Psychiatric Research
1994-1996
Georgetown University
1993-1995
University of Genoa
1989
ALS is a devastating neurodegenerative disorder with no effective treatment. In the present study, we found that daily doses of lithium, leading to plasma levels ranging from 0.4 0.8 mEq/liter, delay disease progression in human patients affected by ALS. None treated lithium died during 15 months follow-up, and was markedly attenuated when compared age-, duration-, sex-matched control riluzole for same amount time. parallel study on genetic animal model, G93A mouse, marked neuroprotection...
Understanding the means by which microglia self-regulate neuroinflammatory response helps modulating their reaction during neurodegeneration. In amyotrophic lateral sclerosis (ALS), classical NF-κB pathway is related to persistent activation and motor neuron injury; however, mechanisms of negative control activity remain unexplored. One major players in termination ubiquitin-editing enzyme A20, has recognized anti-inflammatory functions. Lately, microRNAs are emerging as potent fine-tuners...
Abstract We investigated the involvement of store‐operated channels (SOCs) and transient receptor potential (TRP) in response to activation group I metabotropic glutamate subtype 1 (mGluR1) with agonist (S)‐3,5‐dihydroxyphenylglycine (DHPG, puff application) dopamine neurons rat brain slices. The mGluR1‐induced conductance reversed polarity close 0 mV at more positive potentials when extracellular potassium concentrations were increased, indicating a cationic channel. DHPG currents but not...
In a pilot clinical study that we recently published found lithium administration slows the progression of Amyotrophic Lateral Sclerosis (ALS) in human patients. This was addition with basic (in vitro) and pre-clinical vivo) data demonstrating defect autophagy as final common pathway genesis ALS. fact, used an inducer. detailing protective effects for first time this drug stimulates biogenesis mitochondria central nervous system and, uniquely spinal cord, it induces neuronogenesis neuronal...
We evaluated whether tolerance to the antagonism of bicuculine-induced seizures by diazepam is associated with changes (i) in content mRNAs encoding for gamma-aminobutyric acidA (GABAA) receptor subunits, (ii) expression density these and (iii) 1,4-benzodiazepine binding site characteristics discrete neocortical structures. found that diazepam-tolerant rats, mRNA alpha 1 subunit GABAA decreased frontoparietal motor (FrPaM) cortex hippocampus (42% 20%, respectively) but not somatosensory...
Aging is associated with neurodegenerative processes. 5-Lipoxygenase (5-LO), which also expressed in neurons, the key enzyme synthesis of leukotrienes, inflammatory eicosanoids that are capable promoting neurodegeneration. We hypothesized neuronal 5-LO expression can be up-regulated aging and this may increase brain's vulnerability to observed differences distribution 5-LO-like immunoreactivity various brain areas adult young (2-month-old) vs. old (24-month-old) male rats. Greater was found...
Abstract Neurophysiologic data suggest that orexin neurons are directly excited by ATP through purinergic receptors (P2XR). Anatomical studies, though reporting P2XR in the hypothalamus, did not describe it perifornical hypothalamic area, where orexinergic located. Here we report presence of P2X 2 R subunit rat hypothalamus and demonstrate express R. Double immunohistochemistry showed virtually all orexin‐immunoreactive also immunoreactive, whereas 80% R‐immunoreactive positive....
Neurogenesis in the dentate gyrus of adult hippocampus has been implicated neural plasticity and memory, but molecular mechanisms controlling proliferation differentiation newborn neurons their integration into synaptic circuitry are still largely unknown. To investigate this issue, we have analyzed hippocampal neurogenesis a PC3/Tis21-null mouse model. PC3/Tis21 is transcriptional co-factor endowed with antiproliferative prodifferentiative properties; indeed, its upregulation progenitors...
Amyotrophic lateral sclerosis (ALS) is a motor neuron disease whose pathophysiological deficits, causing impairment in function, are largely unknown. Here we propose that hydrogen sulfide (H2 S), as glial-released inflammatory factor, contributes to ALS-mediated death.H2 S concentrations were analyzed the cerebrospinal fluid of 37 sporadic ALS patients and 14 age- gender-matched controls, tissues familial (fALS) mouse model, spinal cord culture media by means specific innovative...
Amyotrophic Lateral Sclerosis (ALS) is considered the prototype of motor neuron disease, characterized by loss and muscle waste. A well-established pathogenic hallmark ALS mitochondrial failure, leading to bioenergetic deficits. So far, pharmacological interventions for disease have proven ineffective. Trimetazidine (TMZ) described as a metabolic modulator acting on different cellular pathways. Its efficacy in enhancing muscular cardiovascular performance has been widely described, although...
Anxiety disorders are the most common psychiatric disorders. They frequently treated with benzodiazepines, which fast acting highly effective anxiolytic agents. However, their long term use is impaired by tolerance development and abuse liability. In contrast, antidepressants such as selective serotonin reuptake inhibitors (SSRIs) considered first line treatment but have a slow onset of action. Neurosteroids powerful allosteric modulators GABAA glutamate receptors. they also modulate sigma...
Summary: Purpose: We used field‐potential recordings in slices of rat cerebral cortex along with whole‐cell patch from neocortical cells culture to test the hypothesis that antiepileptic drug (AED) lamotrigine (LTG) modulates K + ‐mediated, hyperpolarizing currents. Methods: Extracellular were performed obtained Wistar rats aged 25–50 days. Rat neurons subjected mode voltage clamping under experimental conditions designed study voltage‐gated Results: In vitro slice preparation, LTG (100–400...
Mutations in the gene coding for ubiquitous, anti-oxidant enzyme Cu,Zn superoxide dismutase (SOD1) are associated with familial amyotrophic lateral sclerosis (fALS), a fatal disease characterized by selective loss of motor neurons. Expression mutant SOD1 typical fALS patients restricted to either neurons or astrocytes is insufficient generate pathological phenotype mouse models, suggesting that deleterious interplay between different cell types necessary pathogenesis disease. In this study,...
The present study aims to assess the protective role of antioxidant enzyme catalase (CAT) with relation hydrogen peroxide (H 2 O ) degradation in oxygen plus water on electrophysiological and fluorescence changes induced by vitro ischemia brain damage produced transient vivo ischemia. Neuroprotective effects CAT were determined means recordings confocal microscopy hippocampal slice preparation. Ischemia was simulated oxygen/glucose deprivation (OGD). In middle cerebral artery occlusion...