A5020791097- Neurogenesis and neuroplasticity mechanisms
- Cancer-related gene regulation
- Epigenetics and DNA Methylation
- Genetics and Neurodevelopmental Disorders
- Neuropeptides and Animal Physiology
- Hedgehog Signaling Pathway Studies
- Ubiquitin and proteasome pathways
- Receptor Mechanisms and Signaling
- Neurotransmitter Receptor Influence on Behavior
- interferon and immune responses
- Muscle Physiology and Disorders
- Nuclear Receptors and Signaling
- Pluripotent Stem Cells Research
- Axon Guidance and Neuronal Signaling
- MicroRNA in disease regulation
- Neuroscience and Neuropharmacology Research
- Histone Deacetylase Inhibitors Research
- Nerve injury and regeneration
- Signaling Pathways in Disease
- RNA Research and Splicing
- Cancer-related Molecular Pathways
- Genomics and Chromatin Dynamics
- Neuroinflammation and Neurodegeneration Mechanisms
- Cell Adhesion Molecules Research
- Chemokine receptors and signaling
National Research Council
2015-2024
Institute of Cell Biology and Neurobiology
2014-2023
Fondazione Santa Lucia
1996-2018
National Academies of Sciences, Engineering, and Medicine
2012-2015
Institute of Neurobiology and Molecular Medicine
1996-2012
Stanford University
1989
University of Milan
1982-1988
University of Bern
1985
The p53-inducible gene PC3 (TIS21, BTG2) is endowed with antiproliferative activity. Here we report that expression of in cycling cells induced accumulation hypophosphorylated, growth-inhibitory forms pRb and led to G(1) arrest. This latter was not observed genetic disruption the Rb gene, indicating PC3-mediated arrest dependent. Furthermore, (i) G(1)-S transition exerted by completely rescued coexpression cyclin D1 but A or E; (ii) caused a significant down-regulation protein levels, also...
PC3 is an immediate early gene induced by nerve growth factor in PC12 cells, a cell line derived from tumor of the adrenal medulla that undergoes neuronal differentiation presence factor. This induction independent new protein synthesis as it can occur cycloheximide. also with similar kinetics, but at lower levels, membrane depolarization (both vivo and vitro) epidermal It much levels fibroblast interleukin 6. In found expressed tissues, such brain embryonic day 13.5, placenta, amnion,...
Adult neurogenesis in the dentate gyrus plays a critical role hippocampus-dependent spatial learning. It remains unknown, however, how new neurons become functionally integrated into circuits and contribute to hippocampus-mediated forms of learning memory. To investigate these issues, we used mouse model which differentiation adult-generated can be anticipated by conditionally expressing pro-differentiative gene PC3 (Tis21/BTG2) nestin-positive progenitor cells. In contrast previous studies...
Abstract Physical exercise increases the generation of new neurons in adult neurogenesis. However, only few studies have investigated beneficial effects physical paradigms impaired Here, we demonstrate that running fully reverses deficient neurogenesis within hippocampus and subventricular zone lateral ventricle, observed mice lacking antiproliferative gene Btg1. We also evaluated for first time how influences cell cycle kinetics stem precursor subpopulations wild-type Btg1-null mice, using...
Nerve growth factor (NGF) induces the chromaffin cell line PC12 to differentiate into cells with many of properties sympathetic neurons. We investigated early differentiative phase and identified a gene, PC4, rapidly transiently induced by NGF in cells. PC4 cDNA is homologous partial sequence putative mouse beta-interferon encodes protein related lymphokine, rat gamma-interferon protein. Nonetheless, appears devoid antiviral activity. expressed proliferating differentiating tissues, such as...
LHRH (median eminence) and LH (pituitary plasma) from male female Sprague-Dawley rats were assayed 1 month after streptozotocin injection compared with values in controls either fed ad libitum or offered a restricted diet. Plasma was also stimulation exogenous naloxone. In diabetic males, the median eminence content plasma response to unaltered, pituitary increased, decreased under basal conditions naloxone treatment. females, while reduced, levels not different. Measurements made...
Neurogenesis in the dentate gyrus of adult hippocampus has been implicated neural plasticity and memory, but molecular mechanisms controlling proliferation differentiation newborn neurons their integration into synaptic circuitry are still largely unknown. To investigate this issue, we have analyzed hippocampal neurogenesis a PC3/Tis21-null mouse model. PC3/Tis21 is transcriptional co-factor endowed with antiproliferative prodifferentiative properties; indeed, its upregulation progenitors...
In skeletal muscle cells, the PC4 (Tis7/Ifrd1) protein is known to function as a coactivator of MyoD by promoting transcriptional activity myocyte enhancer factor 2C (MEF2C). this study, we show that up-regulation in vivo adult significantly potentiates injury-induced regeneration enhancing myogenesis. Conversely, observe silencing myoblasts causes delayed exit from cell cycle, accompanied differentiation, and such an effect MyoD-dependent. We provide evidence revealing novel mechanism...
Btg1 belongs to a family of cell cycle inhibitory genes. We observed that is highly expressed in adult neurogenic niches, i.e., the dentate gyrus and subventricular zone (SVZ). Thus, we generated knockout mice analyze role process generation new neurons. Ablation causes transient increase proliferating stem progenitor cells at post-natal day 7; however, 2 months age number these cells, as well mature neurons, greatly decreases compared wild-type controls. Remarkably, Btg1-null exit after...
Growing evidence indicates that cell cycle arrest and neurogenesis are highly coordinated interactive processes, governed by genes neural transcription factors. The gene PC3 ( Tis21 / BTG2 ) is expressed in the neuroblast throughout tube inhibits progression at G 1 checkpoint repressing cyclin D1 transcription. We generated inducible mouse models which expression of was upregulated neuronal precursors cerebellum. These mice exhibited a marked increase production postmitotic neurons...
Retinoic acid controls hematopoietic differentiation through the transcription factor activity of its receptors. They act on specific target genes by recruiting protein complexes that deacetylate or acetylate histones and modify chromatin status. The regulation this process is affected histone methyltransferases, which can inhibit activate depending their amino target. We show here retinoic treatment cells induces expression BTG2. Overexpression increases RARalpha transcriptional response to...
Medulloblastoma, the most common brain tumor in childhood, appears to originate from cerebellar granule cell precursors (GCPs), located external granular layer (EGL) of cerebellum. The antiproliferative gene PC3 (Tis21/BTG2) promotes neurogenesis by inducing GCPs shift proliferation differentiation. To assess whether can prevent neoplastic transformation and medulloblastoma development, we crossed transgenic mice conditionally expressing (TgPC3) with Patched1 heterozygous (Ptc(+/-)), a model...
A failure in the control of proliferation cerebellar granule neuron precursor cells (GCPs), located external granular layer (EGL) cerebellum, gives rise to medulloblastoma. To investigate process neoplastic transformation GCPs, we generated a new medulloblastoma model by crossing Patched1 heterozygous mice, which develop medulloblastomas with low frequency, mice lacking Tis21 gene. Overexpression is known inhibit and trigger differentiation GCPs; its expression decreases human...
Genetic modifications during development of paediatric groups 3 and 4 medulloblastoma are responsible for their highly metastatic properties poor patient survival rates. PRUNE1 is expressed in group 3, which characterized by TGF-β signalling activation, c-MYC amplification, OTX2 expression. We describe the process activation pathway that includes its binding to NME1, upregulation, SNAIL (SNAI1) PTEN inhibition. The newly identified small molecule pyrimido-pyrimidine derivative AA7.1 enhances...