A5048708355- Neuroscience and Neuropharmacology Research
- Parkinson's Disease Mechanisms and Treatments
- Ion channel regulation and function
- Neuroscience and Neural Engineering
- Neurological disorders and treatments
- Neural dynamics and brain function
- Amyotrophic Lateral Sclerosis Research
- Neurotransmitter Receptor Influence on Behavior
- Receptor Mechanisms and Signaling
- Nerve injury and regeneration
- Neuroinflammation and Neurodegeneration Mechanisms
- Neuropeptides and Animal Physiology
- Stress Responses and Cortisol
- Cholinesterase and Neurodegenerative Diseases
- Pain Mechanisms and Treatments
- Alzheimer's disease research and treatments
- Genetic Neurodegenerative Diseases
- Amino Acid Enzymes and Metabolism
- Sleep and Wakefulness Research
- Cardiac electrophysiology and arrhythmias
- Tryptophan and brain disorders
- Neuroscience of respiration and sleep
- MicroRNA in disease regulation
- Photoreceptor and optogenetics research
- Sulfur Compounds in Biology
Fondazione Santa Lucia
2014-2024
Parthenope University of Naples
2016-2024
Research Network (United States)
2024
Istituti di Ricovero e Cura a Carattere Scientifico
2011-2023
Neurological Surgery
1996
University of Washington
1996
University of Milan
1994-1996
Mylan (Switzerland)
1996
Fondazione IRCCS Istituto Neurologico Carlo Besta
1994
Over the last few decades, emerging evidence suggests that non-coding RNAs (ncRNAs) including long-non-coding RNA (lncRNA), microRNA (miRNA) and circular-RNA (circRNA) contribute to molecular events underlying progressive neuronal degeneration, a plethora of ncRNAs have been identified significantly misregulated in many neurodegenerative diseases, Parkinson’s disease synucleinopathy. Although direct link between neuropathology causative candidates has not clearly established cases,...
Abstract Transgenic Huntington's disease (HD) mice, expressing exon 1 of the human HD gene (lines R6/1 and R6/2), are totally resistant to striatal lesions caused by NMDA receptor agonist quinolinic acid (QA). Here we show that this resistance develops gradually over time in both R6/2 it occurred earlier (CAG‐155) than (CAG‐115) mice. The development coincided with appearance nuclear inclusions onset motor deficits. In hippocampal neurons were also QA, especially CA1 region. Importantly,...
Changes in temperature of up to several degrees have been reported different brain regions during various behaviors or response environmental stimuli. We investigated sensitivity dopaminergic neurons the rat substantia nigra pars compacta (SNc), an area important for motor and emotional control, using a combination electrophysiological techniques, microfluorometry, RT-PCR slices. Spontaneous neuron firing, cell membrane potential/currents, intracellular Ca2+ level ([Ca2+]i) were measured...
Abstract Rotenone is a toxin used to generate animal models of Parkinson’s disease; however, the mechanisms toxicity in substantia nigra pars compacta (SNc) neurons have not been well characterized. We investigated rotenone (0.05–1 μ m ) effects on SNc acute rat midbrain slices, using whole‐cell patch‐clamp recording combined with microfluorometry. evoked tolbutamide‐sensitive outward current (94 ± 15 pA) associated increases intracellular [Ca 2+ ] ([Ca i (73.8 7.7 n and [Na + (3.1 0.6 (all...
Abstract We studied the properties of dopaminergic neurons in substantia nigra pars compacta ( SN pc) and ventral tegmental area VTA ) mice expressing enhanced green fluorescent protein eGFP under control tyrosine hydroxylase promoter TH ‐ GFP ). By using a practical map cell positioning distinct pc subregions horizontal midbrain slices we saw that spontaneous firing, membrane properties, body size magnitude hyperpolarization‐activated current I h ‐positive + vary significantly among...
Amyotrophic lateral sclerosis (ALS) is a motor neuron disease whose pathophysiological deficits, causing impairment in function, are largely unknown. Here we propose that hydrogen sulfide (H2 S), as glial-released inflammatory factor, contributes to ALS-mediated death.H2 S concentrations were analyzed the cerebrospinal fluid of 37 sporadic ALS patients and 14 age- gender-matched controls, tissues familial (fALS) mouse model, spinal cord culture media by means specific innovative...
The differentiation of dopaminergic neurons requires concerted action morphogens and transcription factors acting in a precise well-defined time window. Very little is known about the potential role microRNA these events. By performing microRNA-mRNA paired microarray screening, we identified miR-34b/c among most upregulated microRNAs during differentiation. Interestingly, modulates Wnt1 expression, promotes cell cycle exit, induces When combined with ASCL1 NURR1, doubled yield...
Abstract Background The accumulation of α‐synuclein (α‐syn) fibrils in intraneuronal inclusions called Lewy bodies and neurites is a pathological signature Parkinson's disease (PD). Although several aspects linked to α‐syn–dependent pathology (concerning its spreading, aggregation, activation inflammatory neurodegenerative processes) have been under intense investigation, less attention has devoted the real impact α‐syn overexpression on structural functional properties substantia nigra pars...
Amyotrophic lateral sclerosis–Parkinson dementia complex (ALS-PDC) is a neurodegenerative disease with ALS, parkinsonism, and Alzheimer's symptoms that prevalent in the Guam population. β- N -Methylamino alanine (BMAA) has been proposed as toxic agent damaging several neuronal types ALS-PDC, including substantia nigra pars compacta dopaminergic (SNpc DAergic) neurons. BMAA mixed glutamate receptor agonist, but specific pathways activated DAergic neurons are not yet known. We combined...
Metabotropic glutamate receptors modulate neuronal excitability via a multitude of mechanisms, and they have been implicated in the pathogenesis neurodegenerative processes. Here we investigated responses mediated by group I metabotropic (mGluRs) dopamine neurons rat substantia nigra pars compacta, using whole cell patch-clamp recordings combination with microfluorometric measurements [Ca(2+)](i) [Na(+)](i). The selective mGluR agonist (S)-3,5-dihydroxyphenylglycine (3,5-DHPG) was...
Early life stress (ELS) is known to modify trajectories of brain dopaminergic development, but the mechanisms underlying have not been determined. ELS perturbs immune system and microglia reactivity, inflammation influence transmission development. Whether mediate effects on dopamine (DA) development still unknown. We explored repeated early social in male female mice through histological, electrophysiological, transcriptomic analyses. Furthermore, we tested whether these could be mediated...