A5015154029- Adipokines, Inflammation, and Metabolic Diseases
- Immune cells in cancer
- Immune Cell Function and Interaction
- Immune Response and Inflammation
- Adipose Tissue and Metabolism
- MicroRNA in disease regulation
- interferon and immune responses
- Single-cell and spatial transcriptomics
- NF-κB Signaling Pathways
- Nerve injury and regeneration
- Cancer Immunotherapy and Biomarkers
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Ferroptosis and cancer prognosis
- Ovarian function and disorders
- Veterinary medicine and infectious diseases
- Neuroinflammation and Neurodegeneration Mechanisms
- T-cell and B-cell Immunology
- Circular RNAs in diseases
- Tendon Structure and Treatment
- RNA Interference and Gene Delivery
- Inflammasome and immune disorders
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Atherosclerosis and Cardiovascular Diseases
- Mitochondrial Function and Pathology
UConn Health
2018-2024
University of Connecticut
2018-2024
Brigham Young University
2018
Brigham Young University - Idaho
2016
Immune checkpoint inhibitor (ICI) therapy continues to revolutionize melanoma treatment, but only a subset of patients respond. Major efforts are underway develop minimally invasive predictive assays ICI response. Using single-cell transcriptomics, we discovered unique CD8 T cell blood/tumor-shared subpopulation in with high levels oxidative phosphorylation (OXPHOS), the ectonucleotidases CD38 and CD39, both exhaustion cytotoxicity markers. We called this population OXPHOS “CD8+ TOXPHOS...
Adverse cardiovascular events are emerging with the use of immune checkpoint therapies in oncology. Using datasets Trans-Omics for Precision Medicine program (Multi-Ethnic Study Atherosclerosis, Jackson Heart [JHS], and Framingham Study), we examined association plasma proteins each other, their associated protein network high-density lipoprotein cholesterol (HDL-C) low-density (LDL-C), HDL-C- LDL-C-associated networks all-cause mortality risk. Plasma levels LAG3 HAVCR2 showed statistically...
Abstract IL-1 receptor-associated kinase (IRAK) 4 is a central enzyme of the TLR pathways. This study tested hypothesis that IRAK4 activity prerequisite for regulating innate immunity during infections with intracellular bacteria. To this end, we analyzed responses macrophages obtained from mice expressing wild-type (WT) or its kinase-inactive K213M mutant (IRAK4KI) upon infection bacteria Listeria monocytogenes Mycobacterium smegmatis. In contrast to robust induction cytokines by...
MicroRNA-150 (miR-150) has been shown to play a general role in the immune system, but very little is known about its on CD4 + T cell responses. During responses against superantigen Staphylococcal Enterotoxin A, miR-150 expression was down-regulated antigen-specific cells up-regulated CD8 cells. and clonal expansion greater miR-150-KO mice than WT mice, selectively repressed IL-2 production Transcriptome analysis of demonstrated that apoptosis mTOR pathways were highly enriched absence...
T cell receptor (TCR) sensitivity to peptide-major histocompatibility complex (MHC) dictates fate. Canonical models of TCR cannot be fully explained by transcriptional regulation. In this work, we identify a posttranscriptional regulatory mechanism that guides alternative splicing signaling transcripts through an evolutionarily ultraconserved poison exon (PE) in the RNA-binding protein (RBP) TRA2β mouse and human.
Sterile stimuli can trigger inflammatory responses, and in some cases lead to a variety of acute or chronic diseases. In this study, we hypothesize that benzimidazole inhibitor may be used as therapeutic the treatment sterile inflammation. vitro, blocks TLR signalling responses. The does not prevent mouse macrophage activation after stimulation with 2,6,10,14-tetramethylpentadecane (TMPD, also known pristane), hydrocarbon oil mimics features inflammation when injected vivo. However, C57BL/6J...
Schwann cells play a major role in helping heal injured nerves. They help clear debris, produce neurotrophins, upregulate neurotrophin receptors, and form bands of Büngner to guide the healing nerve. But nerves do not always enough neurotrophins receptors repair themselves. Nerve growth factor (NGF) is an important for promoting nerve lysophosphatidylcholine (LPC) has been shown stimulate NGF (NGFR). This study tested administration single intraneural injection LPC (1 mg/mL 10 multiple...
Obesity affects hundreds of millions people worldwide and is characterized by chronic inflammation insulin resistance, leading to Type II diabetes atherosclerotic cardiovascular disease. Extracellular RNAs (exRNAs) are among the components which effect immune actions under obese conditions, technological advances in recent years have rapidly increased our understanding their roles functions. Here we review essential background information on exRNAs vesicles as well impact immune-derived...
Cherubism is a rare autosomal dominant disease caused mainly by P416R point mutation of Src homology 3 Domain Binding Protein 2 (SH3BP2). Though macrophages and osteoblasts were thought to be the major contributors pathology in past, involvement neutrophils cherubism has been demonstrated increasing neutrophil infiltration extensive extracellular trap formation lesion recently. Here, we observed decreased expression lineage marker Ly6G impaired integrin activation on neutrophils, indicating...
Abstract Our current understanding of pathways governing the strength and longevity T cell mediated immune responses primarily focuses on transcriptional epigenetic changes. However, our prior work demonstrated that optimal effector function is also dependent RNA binding proteins (RBPs) carry out post-transcriptional splicing polyadenylation. Here, we find a specific family RBPs, serine- arginine-rich (SR) are significantly upregulated by activation required for CD8 +T responses. SR...
B cells exacerbate obesity-induced metabolic dysfunction and become the second most abundant immune lineage in obese adipose tissue. However, high-resolution strategies to interrogate cellular dynamics driving cell responses under chronic obesity stress are lacking, stifling innovative cell-targeted therapies against health risk. Our previous work established potent role of miR-150 tissue B cells’ (ATB) pro-inflammatory obesity. This project aims elucidate...
Abstract Long non-coding RNAs (lncRNAs) are critical regulators of immune responses, but their role in sepsis is largely unknown. During sepsis, TLR sensing microbes triggers the “cytokines storm”, disseminated intravascular coagulation and organ damage. Patients surviving systemic inflammatory response syndrome develop profound immunosuppression that resembles tolerance often succumb to secondary infections. To study sepsis-associated “cytokine we used vivo exposure mice LPS (endotoxemia)...
<h3>Background</h3> Immune checkpoint blockade (ICB) has greatly advanced the treatment of melanoma. A key component ICB is stimulation CD8+ T cells in tumor. However, therapy only benefits a subset patients and reliable prediction method that does not require invasive biopsies still major challenge field. <h3>Methods</h3> We conducted set comprehensive single-cell transcriptomic analyses peripheral blood (mPBL) tumors (mTIL) from 8 with metastatic <h3>Results</h3> Compared to circulating...
Abstract Interleukin-1 receptor-associated kinase (IRAK) 4 is a serine/threonine involved in TLR/IL-1R responses. Upon activation, autophosphorylation of IRAK4 induces proinflammatory gene transcription. In this study we hypothesize that the activity may be druggable target treatment sterile autoinflammation and Lupus. C57BL/6 female mice injected with hydrocarbon oil Pristane, exhibited significant Pristane-dependent induction splenomegaly; whereas inhibitor reduced inflammatory symptom....