A5033698833- MicroRNA in disease regulation
- Adipokines, Inflammation, and Metabolic Diseases
- Immune cells in cancer
- Immune Cell Function and Interaction
- Cancer-related molecular mechanisms research
- Atherosclerosis and Cardiovascular Diseases
- Adipose Tissue and Metabolism
- Circular RNAs in diseases
- RNA modifications and cancer
- Cancer Cells and Metastasis
- Single-cell and spatial transcriptomics
- RNA Interference and Gene Delivery
- Acute Myeloid Leukemia Research
- Immune Response and Inflammation
- Cell Adhesion Molecules Research
- Cancer Immunotherapy and Biomarkers
- Inflammasome and immune disorders
- Mitochondrial Function and Pathology
- Neonatal and fetal brain pathology
- Extracellular vesicles in disease
- T-cell and B-cell Immunology
- Estrogen and related hormone effects
- Immunotherapy and Immune Responses
- Nuclear Receptors and Signaling
- Cancer Research and Treatments
UConn Health
2016-2025
Universidad Cristiana Autónoma de Nicaragua
2025
University of Connecticut
2016-2024
Texas A&M University
2010-2020
Mitchell Institute
2014-2015
Whitehead Institute for Biomedical Research
2007-2013
Massachusetts Institute of Technology
2008-2013
Inserm
2013
Wuhan University
2012
Renmin Hospital of Wuhan University
2012
The p53 transcription factor is a key tumor suppressor and central regulator of the stress response. To ensure robust precise response to cellular signals, gene expression must be tightly regulated from transcriptional post-translational levels. Computational predictions suggest that several microRNAs are involved in post-transcriptional regulation . Here we demonstrate miR-125b, brain-enriched microRNA, bona fide negative both zebrafish humans. miR-125b-mediated down-regulation strictly...
MicroRNAs (miRNAs) are a family of ≈22-nt noncoding RNAs that can posttranscriptionally regulate gene expression. Several miRNAs specifically expressed in hematopoietic cells. Here we show one such miRNA, miR-150, is mainly the lymph nodes and spleen highly up-regulated during development mature T B cells; expression miR-150 sharply at immature cell stage. Overexpression stem cells, followed by bone marrow transplantation, had little effect on formation either CD8- CD4-positive cells or...
Background— Macrophage activation plays a crucial role in regulating adipose tissue inflammation and is major contributor to the pathogenesis of obesity-associated cardiovascular diseases. On various types stimuli, macrophages respond with either classic (M1) or alternative (M2) activation. M1- M2-mediated signaling pathways corresponding cytokine production profiles are not completely understood. The discovery microRNAs provides new opportunity understand this complicated but network for...
The article describes a readily easy adaptive in vitro model to investigate macrophage polarization. In the presence of GM-CSF/M-CSF, hematopoietic stem/progenitor cells from bone marrow are directed into monocytic differentiation, followed by M1 or M2 stimulation. activation status can be tracked changes cell surface antigens, gene expression and signaling pathways.
AbstractMicroRNAs (miRNAs) are a class of small noncoding RNAs that regulate gene expression at the posttranscriptional level. Research on miRNAs has highlighted their importance in neural development, but specific functions neurally enriched remain poorly understood. We report here profile during neuronal differentiation human neuroblastoma cell line SH-SY5Y. Six were significantly upregulated induced by all-trans-retinoic acid and brain-derived neurotrophic factor. demonstrated ectopic...
MicroRNA miR-125b has been implicated in several kinds of leukemia. The chromosomal translocation t(2;11)(p21;q23) found patients with myelodysplasia and acute myeloid leukemia leads to an overexpression up 90-fold normal. Moreover, is also up-regulated B-cell lymphoblastic carrying the t(11;14)(q24;q32) translocation. To decipher presumed oncogenic mechanism miR-125b, we used transplantation experiments mice. All mice transplanted fetal liver cells ectopically expressing showed increase...
Abstract The identification of mammary gland stem cells (MGSC) or progenitors is important for the study normal breast development and tumorigenesis. Based on their immunophenotype, we have isolated a population mouse that are capable forming “mammospheres” in vitro. Importantly, mammospheres enriched regenerate an entire implantation into fat pad. We also undertook cytogenetic analyses mammosphere-forming after prolonged culture, which provided preliminary insight genomic stability these...
Polarized activation of adipose tissue macrophages (ATMs) is crucial for maintaining function and mediating obesity-associated cardiovascular risk metabolic abnormalities; however, the regulatory network this key process not well defined. Here, we identified a PPARγ/microRNA-223 (miR-223) axis that controls macrophage polarization by targeting distinct downstream genes to shift cellular response various stimuli. In BM-derived macrophages, PPARγ directly enhanced miR-223 expression upon...
Adipose tissue macrophages (ATMs) are crucial for maintaining adipose homeostasis and mediating obesity-induced metabolic abnormalities, including prediabetic conditions type 2 diabetes mellitus. Despite their key functions in regulating immunologic under normal obese conditions, a high-resolution transcriptome annotation system that can capture ATM multifaceted activation profiles has not yet been developed. This is primarily attributed to the complexity of differentiation/activation...
Background and Purpose Approaches to prevent selective progressive loss of insulin‐producing beta cells in Type 1 diabetes mellitus (T1DM) will help manage this prevalent devastating disease. Curcumin (CUR), a natural anti‐inflammatory substance, suppresses diabetes‐associated inflammation cell death. However, very high doses need be used because poor oral bioavailability, making it difficult translate the actions clinical situations. Experimental Approach We have prepared biodegradable...
Immune checkpoint inhibitor (ICI) therapy continues to revolutionize melanoma treatment, but only a subset of patients respond. Major efforts are underway develop minimally invasive predictive assays ICI response. Using single-cell transcriptomics, we discovered unique CD8 T cell blood/tumor-shared subpopulation in with high levels oxidative phosphorylation (OXPHOS), the ectonucleotidases CD38 and CD39, both exhaustion cytotoxicity markers. We called this population OXPHOS “CD8+ TOXPHOS...
Abstract Adipose tissue resident B cells account for more than 20% of stromal within visceral adipose tissues; however, their functions in the niche are poorly elucidated. Here we report that miR-150 modulates function by controlling activation and interactions with other immune cells. miR-150KO mice displayed exacerbated obesity-associated inflammation systemic insulin resistance, which is recapitulated adoptive transfer cells, but not purified immunoglobulin, into obese null mice. Using...
The purpose of this study was to investigate the impact obesity-induced prediabetes/early diabetes on retina provide new evidence pathogenesis type 2 diabetes-associated diabetic retinopathy (DR).A high-fat diet (HFD)-induced obesity mouse model (male C57BL/6J) used in study. At end 12-week HFD feeding regimen, mice were evaluated for glucose and insulin tolerance, retinal light responses recorded by electroretinogram (ERG). Western immunoblot immunohistochemical staining determine changes...
Whereas several interventions can effectively lower lipid levels in people at risk for atherosclerotic cardiovascular disease (ASCVD), event risks remain, suggesting an unmet medical need to identify factors contributing risk. Monocytes and macrophages play central roles atherosclerosis, but studies have yet provide a detailed view of macrophage populations involved increased ASCVD
We have recently demonstrated that nutritional bioactives (fish oil and pectin) modulate microRNA molecular switches in the colon. Since integrated analysis of mRNA expression at an early stage colon cancer development is lacking, this study, four computational approaches were utilized to test hypothesis microRNAs their posttranscriptionally regulated targets, i.e., both total mRNAs actively translated transcripts, are differentially modulated by carcinogen diet treatment. Sprague-Dawley...