A5013303539- MicroRNA in disease regulation
- RNA Research and Splicing
- Erythrocyte Function and Pathophysiology
- RNA modifications and cancer
- Circular RNAs in diseases
- Blood groups and transfusion
- Diabetes Treatment and Management
- RNA and protein synthesis mechanisms
- Blood disorders and treatments
- Yersinia bacterium, plague, ectoparasites research
- Bacteriophages and microbial interactions
- Cardiac, Anesthesia and Surgical Outcomes
- Genetic Syndromes and Imprinting
- RNA regulation and disease
- Genomics and Phylogenetic Studies
- Pancreatitis Pathology and Treatment
- Diabetes and associated disorders
- Advanced biosensing and bioanalysis techniques
- Adipokines, Inflammation, and Metabolic Diseases
- Neuroendocrine Tumor Research Advances
- Immune Cell Function and Interaction
- Bariatric Surgery and Outcomes
- Autophagy in Disease and Therapy
- Microbial infections and disease research
- Blood properties and coagulation
NHS Blood and Transplant
2017-2025
Southwestern Medical Center
2024
The University of Texas Southwestern Medical Center
2018-2024
Thoracic Surgery Foundation
2023
Texas A&M University
2016-2019
MicroRNAs (miRNAs) act in concert with Argonaute (AGO) proteins to repress target messenger RNAs. After AGO loading, miRNAs generally exhibit slow turnover. An important exception occurs when encounter highly complementary targets, which can trigger a process called target-directed miRNA degradation (TDMD). During TDMD, undergo tailing and trimming, suggesting that this is an step the decay mechanism. We identified cullin-RING ubiquitin ligase (CRL), containing substrate adaptor ZSWIM8,...
Abstract Adipose tissue resident B cells account for more than 20% of stromal within visceral adipose tissues; however, their functions in the niche are poorly elucidated. Here we report that miR-150 modulates function by controlling activation and interactions with other immune cells. miR-150KO mice displayed exacerbated obesity-associated inflammation systemic insulin resistance, which is recapitulated adoptive transfer cells, but not purified immunoglobulin, into obese null mice. Using...
Fat storage in adult mammals is a highly regulated process that involves the mobilization of adipocyte progenitor cells (APCs) differentiate to produce new adipocytes. Here we report role for broadly conserved miR-26 family microRNAs (miR-26a-1, miR-26a-2, and miR-26b) as major regulators APC differentiation adipose tissue mass. Deletion all miR-26-encoding loci mice resulted dramatic expansion animals fed normal chow. Conversely, transgenic overexpression miR-26a protected from high-fat...
Loss of function the DIS3L2 exoribonuclease is associated with Wilms tumor and Perlman congenital overgrowth syndrome. LIN28, a oncoprotein, triggers DIS3L2-mediated degradation precursor let-7, microRNA that inhibits development. These observations have led to speculation suppression attributable let-7 regulation. Here we examine new DIS3L2-deficient cell lines mouse models, demonstrating loss has no effect on mature levels. Rather, analysis Dis3l2 -null nephron progenitor cells, potential...
Despite the identification of high-incidence red cell antigen Era nearly 40 years ago, molecular background this antigen, together with other 2 members Er blood group collection, has yet to be elucidated. Whole exome and Sanger sequencing individuals serologically defined alloantibodies identified several missense mutations within PIEZO1 gene, encoding amino acid substitutions extracellular domain Piezo1 mechanosensor ion channel. Confirmation as carrier molecule for antigens was...
MicroRNAs (miRNAs) are post-transcriptional regulators of gene expression that play critical roles in development and disease. Target-directed miRNA degradation (TDMD), a pathway which miRNAs bind to specialized targets with extensive complementarity rapidly decayed, has emerged as potent mechanism controlling levels. Nevertheless, the biological role scope regulation by TDMD mammals remains poorly understood. To address these questions, we generated mice constitutive or conditional deletion...
ATP-grasp superfamily enzymes contain a hand-like ATP-binding fold and catalyze variety of reactions using similar catalytic mechanism. More than 30 protein families are categorized in this superfamily, they involved plethora cellular processes human diseases. Here, we identify C12orf29 (RLIG1) as an atypical enzyme that ligates RNA. Human RLIG1 its homologs autoadenylate on active site Lys residue part reaction intermediate specifically RNA halves containing 5’-phosphate 3’-hydroxyl. binds...
The clinically important MAM blood group antigen is present on haematopoietic cells of all humans except rare MAM-negative individuals. Its molecular basis unknown. By whole-exome sequencing we identify EMP3, encoding epithelial membrane protein 3 (EMP3), as a candidate gene, then demonstrate inactivating mutations in ten known We show that purported tumour suppressor various solid tumours, expressed erythroid cells. Disruption EMP3 by CRISPR/Cas9 gene editing an immortalised human cell line...
Abstract MicroRNAs (miRNAs) are post-transcriptional regulators of gene expression that play critical roles in development and disease. Target-directed miRNA degradation (TDMD), a pathway which miRNAs bind to specialized targets with extensive complementarity rapidly decayed, has emerged as potent mechanism controlling levels. Nevertheless, the biological role scope regulation by TDMD mammals remains poorly understood. To address these questions, we generated mice constitutive or conditional...
Abstract ATP-grasp superfamily enzymes contain a hand-like ATP-binding fold and catalyze variety of reactions using similar catalytic mechanism. More than 30 protein families are categorized in this superfamily, they involved plethora cellular processes human diseases. Here we identify C12orf29 as an atypical enzyme that ligates RNA. Human its homologs auto-adenylate on active site Lys residue part reaction intermediate specifically RNA halves containing 5’-phosphate 3’-hydroxyl. binds tRNA...
As trainees rotate through thoracic subspecialties within their curricula, a crucial portion of robotic training consists actual console operating time. The more time spent on the surgeon console, greater development will be course training. Implementing physician assistant at bedside may increase operative for trainee and develop skills in expeditious rate. objective was to evaluate impact designated can have learning opportunity.
Mutations in GCNT2 have been associated with the rare adult i blood group phenotype or without congenital cataract. We report a novel homozygous frameshift mutation c.1163_1166delATCA, p.(Asn388Argfs*20) as cause of cataract two affected siblings. Blood typing confirmed that both males phenotype, supporting hypothesis partial association I/i and is due to differential expression isoforms.
Proteus mirabilis is a pathogen that has been linked to nosocomial infections. Studies on phages infecting P. may provide therapeutics for infections caused by antibiotic-resistant strains of this pathogen. Here, we announce the complete genome sequence myophage, Mydo, which distantly related Escherichia coli phage rv5.
Searchable abstracts of presentations at key conferences in endocrinology ISSN 1470-3947 (print) | 1479-6848 (online)