A5007259656- Atherosclerosis and Cardiovascular Diseases
- Antioxidant Activity and Oxidative Stress
- Lipoproteins and Cardiovascular Health
- Adipokines, Inflammation, and Metabolic Diseases
- Adipose Tissue and Metabolism
- Cardiovascular Disease and Adiposity
- Protease and Inhibitor Mechanisms
- Peroxisome Proliferator-Activated Receptors
- MicroRNA in disease regulation
- Blood properties and coagulation
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Circular RNAs in diseases
- Cancer-related molecular mechanisms research
- Blood Coagulation and Thrombosis Mechanisms
- Venous Thromboembolism Diagnosis and Management
- Paraoxonase enzyme and polymorphisms
- Cancer, Lipids, and Metabolism
- Nitric Oxide and Endothelin Effects
- Cholesterol and Lipid Metabolism
- Cardiovascular Function and Risk Factors
- RNA modifications and cancer
- Extracellular vesicles in disease
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Nuclear Receptors and Signaling
- Virus-based gene therapy research
KU Leuven
2014-2023
Institute for Atherosclerosis Research
2012-2015
GTx (United States)
2015
Inserm
1997-2009
Sorbonne Université
2000-2009
Park University
2009
Triangle
2009
New York Proton Center
2009
University of Minnesota
2008
Minneapolis Institute of Arts
2008
Background —The association between oxidative modifications of LDL and coronary artery disease (CAD) is suspected but not established. Therefore, the plasma levels oxidized malondialdehyde (MDA)-modified acute syndromes stable CAD was investigated. Methods Results study population contained 63 patients with (45 myocardial infarction 18 unstable angina pectoris), 35 nontransplanted angiographically confirmed angina, 28 heart transplant posttransplant CAD, 79 without 65 control subjects. After...
<h3>Background</h3> The metabolic syndrome is a disorder that includes dyslipidemia, insulin resistance, and hypertension associated with an increased risk of diabetes cardiovascular disease. We determined whether patterns regional fat deposition are in older adults. <h3>Methods</h3> A cross-sectional study was performed included random, population-based, volunteer sample Medicare-eligible adults within the general communities Pittsburgh, Pa, Memphis, Tenn. subjects consisted 3035 men women...
Our aim was to determine the usefulness of circulating oxidized low density lipoprotein (LDL) in identification patients with coronary artery disease (CAD). A total 304 subjects were studied: 178 angiographically proven CAD and 126 age-matched without clinical evidence cardiovascular disease. The Global Risk Assessment Score (GRAS) calculated on basis age, high cholesterol, blood pressure, diabetes mellitus, smoking. Levels LDL measured a monoclonal antibody 4E6-based competition ELISA....
Blood vessels are exposed to multiple mechanical forces that exerted on the vessel wall (radial, circumferential and longitudinal forces) or endothelial surface (shear stress). The stresses strains experienced by arteries influence initiation of atherosclerotic lesions, which develop at regions complex blood flow. In addition, plaque progression eventually rupture is influenced a interaction between biological factors—mechanical regulate cellular molecular composition plaques and,...
Summary The fibrinolytic system was investigated in 120 patients with spontaneous or recurrent deep vein thrombosis (DVT) without any known organic disease able to explain by itself the occurrence of a and defect antithrombin III, Heparin Cofactor II, Protein C, S. assays included: Euglobulin activity (EFA), tissue-type plasminogen activator related antigen (t-PA-Ag) inhibitor (PA inhibitor), which were measured before after 10 min venous occlusion (V. O.). On basis results, could be...
<h3>Context</h3>Experimental data support the hypothesis that oxidized low-density lipoprotein (LDL) is associated with metabolic syndrome. However, this has not been tested in humans.<h3>Objective</h3>To establish relation of LDL syndrome general community.<h3>Design, Setting, and Participants</h3>The Coronary Artery Risk Development Young Adults (CARDIA) study a population-based, prospective, observational study. We studied 1889 participants who were between ages 18 30 years at time...
The object of this study was to establish the association between metabolic syndrome and oxidized LDL (oxLDL) determine risk for coronary heart disease (CHD) in relation levels oxLDL. OxLDL measured plasma from 3,033 elderly participants Health, Aging, Body Composition study. defined according criteria established Third Report National Cholesterol Education Program Expert Panel on Detection, Evaluation, Treatment High Blood Adults. We observed that associated with higher oxLDL due a fraction...
The murine monoclonal antibody mAb-1H11 raised against malondialdehyde (MDA)-modified LDL, was used to detect cross-reacting material in human atheromatous tissue and plasma. MDA-modified LDL levels plasma were 0.19 +/- 0.02 mg/dl (mean SEM) 44 control subjects, 0.24 15 patients with chronic stable angina pectoris (P = NS vs cholesterol matched controls), 1.4 0.1 60 acute myocardial infarction < 0.001 0.86 0.11 22 carotid atherosclerosis controls). Modified isolated from pooled of 10...
OBJECTIVE Although cumulative evidence suggests that increased oxidative stress may lead to insulin resistance in vivo or vitro, community-based studies are scarce. This study examined the longitudinal relationships of biomarkers with development and whether these were independent obesity nondiabetic young adults. RESEARCH DESIGN AND METHODS Biomarkers (F2-isoprostanes [F2Isop] oxidized LDL [oxLDL]), (the homeostasis model assessment [HOMA-IR]), various fatness measures (BMI, waist...
An enzyme-linked immunosorbent assay (ELISA) for the measurement of human tissue-type plasminogen activator (t-PA) was developed. Microtiter plates were coated with a mixture two monoclonal antibodies and bound t-PA quantitated third antibody linked to peroxidase. The lower limit sensitivity 0.2 ng per ml. concentration antigen in citrated plasma subjects found be 3.4 +/- 0.8 ng/ml. had good reproducibility values 3.8, 6.5 4.9 percent respectively intra-, inter-assay inter-dilution variation...
Background —Atherosclerosis is characterized by an early inflammatory response involving proinflammatory mediators such as platelet-activating factor (PAF)-like phospholipids, which are inactivated PAF-acetylhydrolase (PAF-AH). The effect of adenovirus-mediated expression PAF-AH on injury-induced neointima formation and spontaneous atherosclerosis was studied in apolipoprotein E–deficient mice. Methods Results —Intravenous administration adenovirus (5×10 8 plaque-forming units) directing...
Abstract Objective: To investigate whether catch‐up growth after maternal malnutrition would favor the development of obesity in adulthood. Research Methods and Procedures: Pregnant rats were submitted to protein or calorie restriction during course gestation. During lactation, pups protein‐restricted, normally fed, overfed [reduced litter size, control (C) diet]. At weaning, transferred chow a hypercaloric diet (HCD) known induce obesity. Body weight, food intake, blood parameters, glucose...
The metabolic syndrome is typified by obesity, dyslipidemia, diabetes, hypertension, increased oxidative stress, and accelerated atherosclerosis. Paraoxonase1 (PON1), a high-density lipoprotein (HDL)-associated antioxidant enzyme that prevents the oxidation of low-density (LDL), low in syndrome.We used adenovirus-mediated PON1 gene transfer (AdPON1) to overexpress human mice with combined leptin LDL receptor deficiency, model syndrome. activity, plasma lipids, titer autoantibodies against...
Abstract Background Accumulating evidence suggests a cross‐sectional association between oxidative stress and type 2 diabetes (T2D). Systemic stress, as measured by oxidized LDL (oxLDL), has been correlated with visceral fat. We examined the relationship oxLDL, T2D‐ obesity‐related traits in bi‐racial sample of 2985 subjects at baseline after 7 years follow‐up. Methods six T2D‐related (T2D status, HbA 1c , fasting glucose, insulin, adiponectin HOMA‐IR) well (obesity BMI, leptin, % body fat,...
Objective— Obesity is a major risk factor for atherosclerosis and associated with increased cardiovascular morbidity mortality. However, the precise molecular pathways responsible this close association remain poorly understood. Methods Results— In study, we report that leptin-deficiency ( ob/ob ) in low-density lipoprotein receptor knockout ldlr −/− mice induces an unexpected 2.2- to 6-fold reduction atherosclerotic lesion development, compared having similar total cholesterol levels. Ldlr...