A5009885258- Animal Genetics and Reproduction
- Sperm and Testicular Function
- CRISPR and Genetic Engineering
- Reproductive Biology and Fertility
- Mesenchymal stem cell research
- Veterinary Oncology Research
- Xenotransplantation and immune response
- Nitric Oxide and Endothelin Effects
- Angiogenesis and VEGF in Cancer
- Electrospun Nanofibers in Biomedical Applications
- Tissue Engineering and Regenerative Medicine
- Heat shock proteins research
- Reproductive Physiology in Livestock
- Gut microbiota and health
- Essential Oils and Antimicrobial Activity
- Animal Behavior and Welfare Studies
- Virus-based gene therapy research
- Clusterin in disease pathology
- Radiomics and Machine Learning in Medical Imaging
- Effects of Environmental Stressors on Livestock
- Genetic and phenotypic traits in livestock
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Animal Virus Infections Studies
- Molecular Biology Techniques and Applications
- Animal Nutrition and Physiology
University of Bologna
2016-2025
University of Milano-Bicocca
2004
Centro Ricerche Produzioni Animali
1999
Genetic modification of cells and animals is an invaluable tool for biotechnology biomedicine. Currently, integrating vectors are used this purpose. These vectors, however, may lead to insertional mutagenesis variable transgene expression can undergo silencing. Scaffold/matrix attachment region-based nonviral systems that replicate autonomously in mammalian cells, thereby making possible safe reliable genetic higher eukaryotic organisms. In study, genetically modified pig fetuses were...
Endotoxic shock, one of the most prominent causes mortality in intensive care units, is characterized by pulmonary hypertension, systemic hypotension, heart failure, widespread endothelial activation/injury, and clotting culminating disseminated intravascular coagulation multi-organ system failure. In last few years, studies rodents have shown that administration low concentrations carbon monoxide (CO) exerts potent therapeutic effects a variety diseases/disorders. this study, we...
This study was designed to assess the effectiveness of an authorized commercial standardized mixture Artepillin-C, methyl-salicylates, flavonoids and curcuminoids (PHYTO AX'CELL™) provided intermittently during 8 weeks (wk) trial laying hens raised in enriched cages peak production, reduction inflammation status, improving immune response, egg quality. In this study, 764 Lohmann LSL-White on first day (d) 26th wk age (T0), were randomly assigned 2 replicated experimental groups, control...
Multi-gene transgenic pigs would be of benefit for large animal models in medical, agricultural, and pharmaceutical applications; particular xenotransplantation, where extensive genetic manipulation donor is required to make them suitable organ grafting humans. We used the sperm mediated gene transfer (SMGT) method produce with high efficiency multi-gene using three genes coding fluorescent proteins: enhanced blue (EBFP), green (EGFP), red (DsRed2). All proteins were expressed 171 out 195...
ObjectiveThe aim of the present study was to comprehensively evaluate systemic and local inflammation as well progression vascular in normal mechanically injured vessels a large animal model mild hypercholesterolemia. Our also test effect high-dose statin treatment on these processes. MethodsPigs were kept for 120 days standard diet (SD, n=7), high-cholesterol (HCD, n=7) or with Atorvastatin starting after 50 (STATIN, n=7). Left carotid artery balloon injury conducted all groups 60...
ABSTRACT A fundamental role for the endosymbiotic bacteria Wolbachia pipientis in pathogenesis of Dirofilaria immitis infections has emerged recent years. Diagnostic opportunities arising from this breakthrough have not yet been fully exploited. This study was aimed at developing conventional and real-time PCR assays to carry out a molecular survey convenience sample cats living an area where D. is endemic evaluate detection bacterial DNA blood as surrogate assay diagnosing...
Several studies have already described the presence of specialized niches precursor cells in vasculature wall, and it has been shown that these populations share several features with mesenchymal stromal (MSCs). Considering relevance MSCs cardiovascular physiopathology regenerative medicine, usefulness pig animal model this field, we reported a new method for MSC-like cell isolation from aorta. Filling vessel collagenase solution 40 min, all endothelial were detached discarded then treatment...
Vascularization is a crucial factor when approaching any engineered tissue. Vascular wall–mesenchymal stem cells are an excellent in vitro model to study vascular remodeling due their strong angiogenic attitude. This aimed demonstrate the potential of experimental highly porous scaffolds based on polylactic acid (PLA) or poly-e-caprolactone (PCL) doped with calcium silicates (CaSi) and dicalcium phosphate dihydrate (DCPD), namely PLA-10CaSi-10DCPD PCL-10CaSi-10DCPD, designed for regeneration...
Doxorubicin (DOX) is widely used in both human and veterinary oncology although the onset of multidrug resistance (MDR) neoplastic cells often leads to chemotherapy failure. Better understanding cellular mechanisms that circumvent efficacy paramount. The aim this study was investigate response two canine mammary tumour cell lines, CIPp from a primary CIPm, its lymph node metastasis, exposure EC50(20h) DOX at 12, 24 48 h treatment. We assessed uptake subcellular distribution DOX, expression...
The metacarpophalangeal joint undergoes enormous loading during locomotion and can therefore often become inflamed, potentially resulting in osteoarthritis (OA). There are studies indicating that the endocannabinoid system (ECS) modulates synovium homeostasis, could be a promising target for OA therapy. Some cannabinoid receptors, which modulate proliferative secretory responses inflammation, have been functionally identified human animal synovial cells.To characterize cellular distribution...
The value of pig as "large animal model" is a well-known tool for translational medicine, but it can also be beneficial in studying health one-health vision. ConcePTION Project aims to provide new information about the risks associated with medication use during breastfeeding, this not available most commonly used drugs. In IMI-Conception context, Göttingen Minipigs have been preferred hybrid pigs their genetic stability and microbiological control. For first time, present research, three...
Abstract The aim of the present study was to investigate influence 50 Hz sinusoidal magnetic field on Hsp27, Hsp70, and Hsp90 expression in a model primary culture porcine aortic endothelial cells (PAEC). We took into consideration Hsp profile terms mRNA expression, protein localization inside cells. choice cell system motivated by involvement onset many diseases; moreover, only few reports describe effects extremely low frequency fields (ELF‐MFs) such ELF‐MF exposure induced an increase...
Recent findings suggest that progenitor and multipotent mesenchymal stromal cells (MSCs) are associated with vascular niches. Cells displaying properties differentiating to whole components of a functional blood vessel, including endothelial smooth muscle cells, can be defined as stem (VSCs). Recently, we isolated population porcine aortic precursor (pAVPCs), which have MSC- pericyte-like properties. The aim the present work was investigate whether pAVPCs possess VSC-like assess their...
Intense exercise causes to organisms have oxidative stress and inflammation at the gastrointestinal (GI) level. The reduction in intestinal blood flow exercise-linked thermal damage mucosa can cause barrier disruption, followed by an inflammatory response. Furthermore, adaptation may affect gut microbiota metabolome of biofluids. aim present research was evaluate presence a GI derangement hunting dogs through non-invasive sampling as consequence period intense comparison with samples...