A5011842156- Antiplatelet Therapy and Cardiovascular Diseases
- Lipoproteins and Cardiovascular Health
- Venous Thromboembolism Diagnosis and Management
- Connective tissue disorders research
- Folate and B Vitamins Research
- Aortic Disease and Treatment Approaches
- Atrial Fibrillation Management and Outcomes
- Aortic aneurysm repair treatments
- Acute Myocardial Infarction Research
- Acute Ischemic Stroke Management
- Cardiac Valve Diseases and Treatments
- Blood Coagulation and Thrombosis Mechanisms
- Diet and metabolism studies
- Protease and Inhibitor Mechanisms
- Platelet Disorders and Treatments
- Nutritional Studies and Diet
- Coronary Interventions and Diagnostics
- Inflammatory mediators and NSAID effects
- Muscle Physiology and Disorders
- Cell Adhesion Molecules Research
- Diabetes Treatment and Management
- Heparin-Induced Thrombocytopenia and Thrombosis
- Metabolomics and Mass Spectrometry Studies
- Blood properties and coagulation
- Atherosclerosis and Cardiovascular Diseases
University of Florence
2016-2025
Azienda Ospedaliero-Universitaria Careggi
2016-2025
Azienda Sanitaria di Firenze
2024
Ospedale Santa Maria
2024
Don Carlo Gnocchi Foundation
2009-2021
Ospedale Policlinico San Martino
2017
Società Italiana di Cardiologia
2017
Vanderbilt University
2017
University of Pisa
2016
University of Bologna
2014
Background— The clinical impact of platelet aggregation assessed by point-of-care assays is unknown. We sought to evaluate whether high residual reactivity (RPR) ADP during clopidogrel therapy, measured a assay, predicts adverse events in acute coronary syndrome patients undergoing percutaneous intervention. Methods and Results— used the VerifyNow P2Y12 assay (Accumetrics Inc, San Diego, Calif) determine RPR 683 with dual-antiplatelet therapy who underwent intervention bare-metal or...
High residual platelet reactivity (HRPR) in patients receiving clopidogrel has been associated with high risk of ischemic events after percutaneous coronary intervention (PCI).To test the hypothesis that HRPR loading is an independent prognostic marker long-term thrombotic acute syndromes (ACS) undergoing invasive procedure and antithrombotic treatment adjusted according to results function tests.Prospective, observational, referral center cohort study 1789 consecutive ACS PCI from April...
Abstract We developed a novel software tool, EXCAVATOR, for the detection of copy number variants (CNVs) from whole-exome sequencing data. EXCAVATOR combines three-step normalization procedure with heterogeneous hidden Markov model algorithm and calling method that classifies genomic regions into five states. validate on three datasets compare results other methods. These analyses show outperforms methods is therefore valuable tool investigation CNVs in largescale projects, as well clinical...
Ullrich syndrome is a recessive congenital muscular dystrophy affecting connective tissue and muscle. The molecular basis unknown. Reverse transcription–PCR amplification performed on RNA extracted from fibroblasts or muscle of three patients followed by heteroduplex analysis displayed heteroduplexes in one the genes coding for collagen type VI (COL6). In patient A, we detected homozygous insertion C leading to premature termination codon triple-helical domain COL6A2 mRNA. Both healthy...
The aim of this study was to evaluate the effect polymorphisms affecting clopidogrel metabolism (CYP3A4 IVS10+12G/A and CYP2C19*2) P2Y12 receptor (P2Y12 T744C) on modulating platelet function in acute coronary syndrome patients dual antiplatelet treatment.Residual reactivity (RPR) phenomenon therapy requires clarification. T744C, CYP3A4 and, healthy individuals only, CYP2C19*2 have been investigated; however, influence a large population high-risk vascular treatment has not yet elucidated.A...
BACKGROUND Blood clotting activation is an important component of the inflammatory response; outbursts unstable angina are usually associated with increased thrombin formation and coronary mural thrombosis. METHODS AND RESULTS To investigate 1) whether monocyte responsible for enhanced during bursts 2) what mechanism(s) might be activation, we studied patients (n = 31), stable effort 23), left endoventricular thrombosis 8), control subjects 44), measuring plasma fibrinopeptide A (FPA) levels...
The nanopore sequencing process is based on the transit of a DNA molecule through nanoscopic pore, and since 90s considered as one most promising approaches to detect polymeric molecules. In 2014, Oxford Nanopore Technologies (ONT) launched beta-testing program that supplied scientific community with first prototype sequencer: MinION. Thanks this program, several research groups had opportunity evaluate performance novel instrument develop computational for analyzing new generation data....
Abstract Motivation: Runs of homozygosity (ROH) are sizable chromosomal stretches homozygous genotypes, ranging in length from tens kilobases to megabases. ROHs can be relevant for population and medical genetics, playing a role predisposition both rare common disorders. commonly detected by single nucleotide polymorphism (SNP) microarrays, but attempts have been made use whole-exome sequencing (WES) data. Currently available methods developed the analysis uniformly spaced SNP-array maps do...
Among plasma proteins, fibrinogen represents a major target of oxidative modifications. In patients with post-acute myocardial infarction (6 months after the acute event), oxidation-induced carbonyls and function were estimated using in vitro ex vivo approaches. Fibrinogen structural features clot architecture also explored.In 39 28 age-, sex-, risk factor-matched controls, stress markers (in purified fractions), thrombin-catalyzed fibrin polymerization, plasmin-induced lysis estimated....
Copy Number Variants (CNVs) are structural rearrangements contributing to phenotypic variation that have been proved be associated with many disease states. Over the last years, identification of CNVs from whole-exome sequencing (WES) data has become a common practice for research and clinical purpose and, consequently, demand more efficient accurate methods increased. In this paper, we demonstrate than 30% WES map outside targeted regions these reads, usually discarded, can exploited...
Abstract Aims Clopidogrel is prescribed for the prevention of atherothrombotic events. While investigations have identified genetic determinants inter-individual variability in on-treatment platelet inhibition (e.g. CYP2C19*2), evidence that these variants clinical utility to predict major adverse cardiovascular events (CVEs) remains controversial. Methods and results We assessed impact 31 candidate gene polymorphisms on adenosine diphosphate (ADP)-stimulated reactivity 3391...
COVID-19 has been associated with a broad range of long-term sequelae, commonly referred to as “long-COVID” or “post-COVID-19” syndrome. Despite an increasing body literature, long COVID remains poorly characterized. We retrospectively analysed data from electronic medical records patients admitted the post-COVID-19 outpatient service Infectious and Tropical Diseases Unit, Careggi University Hospital, Florence, Italy, between June 2020 2021, 4–12 weeks after hospital discharge. A total 428...
Few and contrasting data are available on the prevalence of hemostatic risk factors in patients with central retinal vein occlusion (CRVO). Aim this study was to investigate metabolic inherited for venous thrombosis 100 CRVO (age: 59 yrs; range 18-77) controls 56 18-84). In homocysteine (Hcy) levels were significantly higher than affected by C677T methylenetetrahydrofolate reductase (MTHFR) polymorphism (p < 0.001). The prevalences activated protein C resistance (APCR), factor V Leiden...
Bicuspid aortic valve (BAV) is the most frequent congenital heart disease with involvement in thoracic dilatation, aneurysm and dissection. Although BAV Marfan syndrome (MFS) share some clinical features, MFS patients display mutations FBN1, gene encoding fibrillin-1, genetic background of isolated poorly defined. Ten consecutive [8 men, age range 24–42 years] without were clinically characterized. phenotype function, together evaluation morphology, comprehensively assessed by Doppler...
Background and Purpose— Experimentally, matrix metalloproteinases (MMPs) play a detrimental role related to hemorrhagic transformation severity of an ischemic brain lesion. Tissue-type plasminogen activator (tPA) enhances such effects. This study aimed expand clinical evidence in this connection. Methods— We measured MMPs 1, 2, 3, 7, 8, 9, tissue inhibitors 4 circulating level blood taken before 24 hours after tPA from 327 patients (mean age, 68.9±12.1 years; median National Institutes...