The root-hair pattern of Arabidopsis is determined through a regulatory circuit composed transcription factor genes. homeobox gene GLABRA2 (GL2) has been considered key component, acting farthest downstream in this regulation. GL2 modified to include transactivating function caused epidermal cells develop ectopic root hairs or hair-like structures. With system, the phospholipase Dzeta1 (AtPLDzeta1) was identified as direct target GL2. Inducible expression AtPLDzeta1 promoted initiation. We...

The temporal and spatial expression of one member the Arabidopsis 1-aminocyclopropane-1-carboxylate (ACC) synthase gene family (ACS1) was analyzed using a promoter-[beta]-glucuronidase fusion. ACS1 is under developmental control both in shoot root. High observed young tissues switched off mature tissues. promoter activity strongly correlated with lateral root formation. Dark-grown seedlings exhibited different pattern from light-grown ones. ACC content vivo oxidase were determined. activity....

A genomic clone of one member the Arabidopsis thaliana (L.) Heynh. 1-aminocyclopropane-1-carboxylate (ACC) synthase (S-adenosyl-L-methionine methylthioadenosine-lyase, EC 4.4.1.14) gene family (AT-ACC1) was isolated and sequenced. region homology found in 5'-untranslated with promoter a zucchini tomato ACC gene. Comparison its primary structure other synthases revealed conservation seven peptide regions as well similarity 11 amino acids catalytic site aminotransferases. Genomic DNA gel...

Polyamines are involved in key developmental processes and stress responses. Copper amine oxidases oxidize the polyamine putrescine (Put), producing an aldehyde, ammonia, hydrogen peroxide (H2O2). The Arabidopsis (Arabidopsis thaliana) oxidase gene At4g14940 (AtAO1) encodes apoplastic copper expressed at early stages of vascular tissue differentiation roots. Here, its role root development xylem was explored by pharmacological forward/reverse genetic approaches. Analysis AtAO1 expression...

Copper-containing amine oxidases (CuAOs) catalyze polyamines (PAs) terminal oxidation producing ammonium, an aminoaldehyde and hydrogen peroxide (H2O2). Plant CuAOs are induced by stress-related hormones, methyl-jasmonate (MeJA), abscisic acid (ABA) salicylic (SA). In the Arabidopsis genome, eight genes encoding have been identified. Here, a comprehensive investigation of expression pattern four AtCuAOs from α γ phylogenetic subfamilies, two peroxisomal AtCuAOα2 (At1g31690) AtCuAOα3...

Plant copper amine oxidases (CuAOs) are involved in wound healing, defense against pathogens, methyl-jasmonate-induced protoxylem differentiation, and abscisic acid (ABA)-induced stomatal closure. In the present study, we investigated role of Arabidopsis thaliana CuAOδ (AtCuAOδ; At4g12290) ABA-mediated closure by genetic pharmacological approaches. Obtained data show that AtCuAOδ is up-regulated ABA two Atcuaoδ T-DNA insertional mutants less responsive to this hormone, showing reduced H2O2...

We have isolated and sequenced a partial tomato alcohol dehydrogenase ( Adh ) cDNA clone. Expression of was studied at the messenger RNA level in seedlings, roots, fruit. High induction observed under hypoxic conditions, both seedlings roots. In addition, mRNA present mature green pink stage fruit, highly induced late ripening. Moreover, an artificial ripening treatment resulted least 50‐fold compared to level. Genomic DNA gel blotting suggested presence multigene family for tomato.

Saporins are type 1 ribosome-inactivating proteins (RIPs: EC 3.2.2.22) produced in various organs of Saponaria officinalis L. Two distinct saporin types, saporin-L and saporin-S isoforms, were respectively purified from the intra- extra-cellular fractions soapwort leaves. The isoform was lowly identical, differed for toxicity, molecular mass amino acid composition forming a new monophyletic group. Genes encoding both L- S-type isoforms cloned leaf-specific cDNA library; encoded products...

Copper amine oxidases oxidize the polyamine putrescine to 4-aminobutanal with production of plant signal molecule hydrogen peroxide (H2O2) and ammonia. The Arabidopsis (Arabidopsis thaliana) gene At4g14940 (AtAO1, previously referred as ATAO1) encodes an apoplastic copper oxidase expressed in lateral root cap cells developing xylem, especially protoxylem metaxylem precursors. In our recent study, we demonstrated that AtAO1 expression is strongly induced vascular tissues by wound-signal...

The Arabidopsis gene AtCuAOβ (At4g14940) encodes an apoplastic copper amine oxidase (CuAO) highly expressed in guard cells of leaves and flowers root vascular tissues, especially protoxylem metaxylem precursors, where its expression is strongly induced by the wound signal methyl jasmonate (MeJA). hydrogen peroxide (H2O2) derived AtCuAOβ-driven oxidation substrate putrescine (Put), mediates MeJA-induced early differentiation. Considering that maturation was also both exogenous Put leaf...

The Arabidopsis thaliana (L.) Heynh. 1‐aminocyclopropane‐1‐carboxylate synthase gene 1 ( AT‐ACS1 ) was found to be developmentally regulated with pronounced activity in immature tissues. effect of different hormones on this expression pattern studied by using a transgenic line carrying the β ‐glucuronidase GUS under control promoter. In addition, level mRNA for assayed ethylene and auxin‐insensitive backgrounds, as well an auxin homeostasis mutant competitive reverse transcriptase polymerase...

Root architecture and xylem phenotypic plasticity influence crop productivity by affecting water nutrient uptake, especially under those environmental stress, which limit supply or imply excessive losses. Xylem maturation depends on coordinated events of cell wall lignification developmental programmed death (PCD), could both be triggered developmental- and/or stress-driven hydrogen peroxide (H₂O₂) production. Here, the effect wounding cotyledonary leaf root protoxylem was explored in...

Download This Paper Open PDF in Browser Add to My Library Share: Permalink Using these links will ensure access this page indefinitely Copy URL DOI