A5054183574- Polysaccharides and Plant Cell Walls
- Plant-Microbe Interactions and Immunity
- Biofuel production and bioconversion
- Enzyme Production and Characterization
- Algal biology and biofuel production
- Plant Pathogenic Bacteria Studies
- Energy Efficient Wireless Sensor Networks
- Plant tissue culture and regeneration
- Microbial Metabolic Engineering and Bioproduction
- Plant Stress Responses and Tolerance
- Indoor and Outdoor Localization Technologies
- Enzyme-mediated dye degradation
- Photosynthetic Processes and Mechanisms
- Dynamics and Control of Mechanical Systems
- Antenna Design and Optimization
- Enzyme Catalysis and Immobilization
- Legume Nitrogen Fixing Symbiosis
- Microbial Metabolites in Food Biotechnology
- Transgenic Plants and Applications
- Smart Agriculture and AI
- Plant Toxicity and Pharmacological Properties
- Structural Response to Dynamic Loads
- Plant pathogens and resistance mechanisms
- Flexible and Reconfigurable Manufacturing Systems
- Millimeter-Wave Propagation and Modeling
University of L'Aquila
2019-2025
University of Verona
2018-2020
Sapienza University of Rome
2005-2019
Istituto Pasteur
2011
University of Trento
2003-2010
UCB Pharma (Belgium)
2007
Centro Científico Tecnológico - San Juan
1988
National University of Mar del Plata
1986
Battelle
1977
Significance Damage-associated molecular patterns (DAMPs), released from host tissues as a consequence of pathogen attack, have been proposed endogenous activators immune responses in both animals and plants. Oligogalacturonides (OGs), oligomers α-1,4–linked galacturonic acid generated vitro by the partial hydrolysis pectin, shown to function potent elicitors immunity when they are applied exogenously plant tissues. However, there is no direct evidence that OGs can be produced vivo or...
The localisation of moving and transceiver-free objects is addressed by processing the Received Signal Strength Indicator (RSSI) available at nodes a wireless sensor network. Starting from RSSI measurements, probability presence unknown mobile determined means customised classification approach based on support vector machine. Experimental results assess feasibility proposed approach.
Summary Recognition of endogenous molecules acting as ‘damage‐associated molecular patterns’ (DAMPs) is a key feature immunity in both animals and plants. Oligogalacturonides (OGs), i.e. fragments derived from the hydrolysis homogalacturonan, major component pectin are well known class DAMPs that activate protect plants against several microbes. However, hyper‐accumulation OGs severely affects growth, eventually leading to cell death clearly pointing players growth‐defence trade‐off. Here we...
Summary The plant cell wall is the barrier that pathogens must overcome to cause a disease, and this end they secrete enzymes degrade various components. Due complexity of these components, several types oligosaccharide fragments may be released during pathogenesis some can act as damage‐associated molecular patterns ( DAMP s). Well‐known s are oligogalacturonides OG s) upon degradation homogalacturonan products cellulose breakdown, i.e. cellodextrins CD We have previously reported four...
Culturing microalgae using dairy-wastes offers the opportunity of producing valuable biomass for different industrial applications. The capability four Chlorella species and a recombinant Chlamydomonas reinhardtii strain to mixotrophically grow in wastewaters from an Italian dairy factory was investigated. A robust algal growth could be efficiently sustained these wastes, despite abundance d-Lactose (~4% w/v), that not metabolized by any microalgal species. Non axenic cocultivation together...
Microalgae are efficient producers of lipid-rich biomass, making them a key component in developing sustainable energy source, and an alternative to fossil fuels. Chlorella species special interest because their fast growth rate photobioreactors. However, biological constraints still cast significant gap between the high cost biofuel cheap oil, thus hampering perspective producing CO2-neutral biofuels. A issue is inefficient use light caused by its uneven distribution culture that generates...
The enzymatic hydrolysis of cell wall polysaccharides results in the production oligosaccharides with nature damage-associated molecular patterns (DAMPs) that are perceived by plants as danger signals. vitro oxidation oligogalacturonides and cellodextrins plant FAD-dependent oligosaccharide-oxidases (OSOXs) suppresses their elicitor activity vivo, suggesting a protective role OSOXs against prolonged activation defense responses potentially deleterious for health. However, also produced...
Plant immunity is triggered by endogenous elicitors known as damage-associated molecular patterns (DAMPs). Oligogalacturonides (OGs) are DAMPs released from the cell wall (CW) demethylated homogalacturonan during microbial colonization, mechanical or pest-provoked damage, and physiological CW remodeling. Berberine bridge enzyme-like (BBE-l) proteins named OG oxidases (OGOXs) oxidize inactivate OGs to avoid deleterious growth-affecting hyper-immunity possible death. Using OGOX1...
Plants have evolved various resistance mechanisms to cope with biotic stresses that threaten their survival. The BBE23 member (At5g44360/BBE23) of the Arabidopsis berberine bridge enzyme-like (BBE-l) protein family (Arabidopsis thaliana) has been characterized in this paper parallel closely related and previously described CELLOX (At4g20860/BBE22). In addition cellodextrins, both enzymes, renamed here as CELLODEXTRIN OXIDASE 2 1 (CELLOX2 CELLOX1), respectively, oxidize mixed-linked...
Abstract We report here the low-resolution structure of complex formed by endo-polygalacturonase from Fusarium phyllophilum and one polygalacturonase-inhibiting protein Phaseolus vulgaris after chemical cross-linking as determined small-angle x-ray scattering analysis. The inhibitor engages its concave surface leucine-rich repeat domain with enzyme. Both sides enzyme active site cleft interact inhibitor, accounting for competitive mechanism inhibition observed. is in agreement previous...
Oligogalacturonide (OG)-oxidase 1 (OGOX1) and cellodextrin (CD)-oxidase (CELLOX) are plant berberine bridge enzyme-like oligosaccharide oxidases that oxidize OGs CDs, cell-wall fragments with the nature of damage-associated molecular patterns. The oxidation CDs attenuates their elicitor activity concomitantly releases H2O2. By using a multiple enzyme-based assay, we demonstrate H2O2 generated downstream combined action between fungal polygalacturonase OGOX1 or an endoglucanase CELLOX can be...
Driven by economic and environmental pressures, precision agriculture has brought many technological enhancements to traditional farm machinery management tools. For a small tillage, manual control of inputs (such as water, fertilizer, pesticide) is still possible, but such an approach becomes unfeasible for larger cultivations. Furthermore, the based on operator's opinion often have no quantitative basis. In order optimize yield use available resources, wireless sensor networks can play...
In the last two decades, wide diffusion of mobile phones and wireless technologies has brought many advantages in professional activities as well everyday life. general, communication networks are constituted by a limited number base stations. The spatial distribution field radiated from stations turns out to be non-uniform with variations both time space that can exceed normative limits electromagnetic emissions. order real-time long-term monitor emissions, an innovative low cost solution...
Energy demand is constantly growing, and, nowadays, fossil fuels still play a dominant role in global energy production, despite their negative effects on air pollution and the emission of greenhouse gases, which are main contributors to warming. An alternative clean source represented by lignocellulose fraction plant cell walls, most abundant carbon Earth. To obtain biofuels, must be efficiently converted into fermentable sugars. In this regard, exploitation wall lytic enzymes (CWLEs)...
Abstract Oligogalacturonide-oxidases (OGOXs) and cellodextrin-oxidase (CELLOX) are plant berberine bridge enzyme-like oligosaccharide-oxidases (OSOXs) that oxidize, respectively, oligogalacturonides (OGs) cellodextrins (CDs), thereby inactivating their elicitor nature concomitantly releasing H 2 O . Little is known about the physiological role of OSOX activity. By using an ABTS ·+ -reduction assay, we identified a novel reaction mechanism through which activity OSOXs on cell wall...
ABSTRACT Plant immunity is triggered by endogenous elicitors known as damage-associated molecular patterns (DAMPs). Oligogalacturonides (OGs) are DAMPs released from the cell wall (CW) demethylated homogalacturonan during microbial colonization, mechanical or pest-provoked tissue damage, and physiological CW remodeling. Berberine Bridge Enzyme-like (BBE-l) proteins named OG oxidases (OGOXs) have been proposed to govern OGs homeostasis, which necessary avoid deleterious growth-affecting...