A5033137470- Plant Virus Research Studies
- Plant Pathogenic Bacteria Studies
- Phytoplasmas and Hemiptera pathogens
- Plant and Fungal Interactions Research
- Insect-Plant Interactions and Control
- Transgenic Plants and Applications
- Plant Pathogens and Fungal Diseases
- Poxvirus research and outbreaks
- Herpesvirus Infections and Treatments
- Plant Reproductive Biology
- Plant Disease Resistance and Genetics
- Cocoa and Sweet Potato Agronomy
- Plant tissue culture and regeneration
- Insect symbiosis and bacterial influences
- Plant Physiology and Cultivation Studies
- Bacteriophages and microbial interactions
- Plant-Microbe Interactions and Immunity
- Animal Virus Infections Studies
- Insect and Pesticide Research
- Plant pathogens and resistance mechanisms
- Mosquito-borne diseases and control
- Plant Pathogens and Resistance
- CRISPR and Genetic Engineering
- Fungal Plant Pathogen Control
- Toxin Mechanisms and Immunotoxins
Instituto Valenciano de Investigaciones Agrarias
2012-2024
Centro de Investigación y Tecnología Agroalimentaria de Aragón
2002
Biologie du Fruit et Pathologie
1998
Universidad de La Laguna
1993
Since first being recorded in 1917–18 Bulgaria, sharka (plum pox) disease has progressively spread via infected plant material to be present most Prunus ‐growing nations today. The serious agronomic and political consequences because it causes enormous economic losses. In countries which is endemic, a high percentage of apricot European plum production unmarketable the disease. To these figures should added costs sanitary controls, surveys eradication programmes against virus. Estimated...
A new symptomatology was observed in celery (Apium graveolens) Villena, Spain 2008. Symptomatology included an abnormal amount of shoots per plant and curled stems. These vegetative disorders were associated with 'Candidatus Liberibacter solanacearum' not phytoplasmas. Samples from sap immobilized on membranes based the spot procedure tested using a newly developed real-time polymerase chain reaction assay to detect 'Ca. L. solanacearum'. Then, test kit validated by intralaboratory assays...
Eleven monoclonal antibodies specific to plum pox potyvirus (PPV) coat protein were obtained by hybridoma technology from Spanish PPV isolates. In addition, two for cylindrical inclusions (CIP non‐structural proteins) obtained. The assayed DASI ELISA against 81 At least nine different epitopes found and 21 distinct serological patterns of reaction (serogroups) established using selected the collection isolates, indicating high variability among Changes in epitope composition observed after...
In São Paulo State, Brazil, 'Candidatus Liberibacter americanus' and asiaticus' are associated with huanglongbing (HLB). Affected municipalities occur mainly in the central southern regions, where annual number of hours above 30°C is two to five times lower than that extreme northern western regions. The influence temperature on sweet orange trees infected 'Ca. L. or was studied temperature-controlled growth chambers. Symptom progression new shoots naturally experimentally graft-inoculated...
Summary Monoclonal antibodies (MAb) specific for citrus tristeza virus (CTV) were obtained from hybrid cells produced by fusion of a non-secreting myeloma cell line with spleen BALB/c mice immunized isolate T-308 CTV. Three MAb characterized their immunoglobulin isotype and titres in culture ascites fluids. Each was conjugated alkaline phosphatase used double-antibody sandwich enzyme-linked immunosorbent assay When tested against 23 strains CTV, each recognized all the uniform reactions low...
A direct tissue blot immunoassay (DTBIA) procedure was tested for detection of citrus tristeza virus (CTV).Freshly cut stem, petiole or fruit pedicel carefully pressed to nitrocellulose membranes.The membranes were blocked by incubation in dilute bovine serum albumin and then incubated with unlabeled biotinylated monoclonal polyclonal antibodies.Antigen-bound antibodies detected exposure a streptavidin-alkaline phosphatase conjugate (APC) antigen-bound goat anti-mouse anti-rabbit IgG-APC.The...
In Brazil 'Candidatus Liberibacter asiaticus' and 'Ca. L. americanus' cause huanglongbing (also known as greening), the most destructive citrus disease. A shift in pathogen prevalence was observed over time, with a disproportional increase occurrence. Graft transmission experiments were used for comparative study of both species using budsticks from symptomatic branches field-affected trees inoculum. The plants inoculated or alone, simultaneously species. Symptom manifestation conventional...
Plum pox potyvirus (PPV) isolates may be divided into four groups separated by serological, molecular, and epidemiological differences. Monoclonal antibodies specific for the two major of isolates, represented D M serotypes virus, have been obtained. Polymerase chain reaction (PCR)-based assays allowing direct detection differentiation PPV also developed. We now report on a large-scale comparison these typing approaches. The results obtained show an overall excellent correlation between in...
Transgenic clones C2, C3, C4, C5, C6, and PT-6, of plum (Prunus domestica L.) transformed with the coat protein (CP) gene Plum pox virus (PPV), PT-23 marker genes only, nontransgenic B70146 were evaluated for sharka resistance under high infection pressure in field trials Poland Spain. These sites differed climatic conditions isolates. clone C5 showed to PPV at both sites. None trees became naturally infected by aphids during seven (Spain) or eight (Poland) years test, although up 100% other...
A protocol for the specific detection and quantification of ‘ Candidatus Liberibacter solanacearum’ in carrot seeds using real‐time PCR was developed. The bacterium detected 23 out 54 seed lots from 2010 to 2014, including collected diseased mother plants. average total number Ca . L. cells individual ranged 4·8 ± 3·3 210 6·7 per three lots, but propidium monoazide target live cells, 95% one lot were found be dead. Liberibacter‐like observed phloem sieve tubes coat leaf midrib seedlings. as...