A5103252766- Mitochondrial Function and Pathology
- Genetics, Aging, and Longevity in Model Organisms
- Coenzyme Q10 studies and effects
- Autophagy in Disease and Therapy
- Adipose Tissue and Metabolism
- ATP Synthase and ATPases Research
- Metabolism and Genetic Disorders
- Microfluidic and Capillary Electrophoresis Applications
- Diet and metabolism studies
- Plant Virus Research Studies
- Physiological and biochemical adaptations
- Sirtuins and Resveratrol in Medicine
- Biochemical effects in animals
- Animal Virus Infections Studies
- Inflammasome and immune disorders
- Birth, Development, and Health
- Bone health and osteoporosis research
- Vitamin D Research Studies
- Biochemical Acid Research Studies
- Viral Infections and Immunology Research
- Lipid metabolism and biosynthesis
- Calcium signaling and nucleotide metabolism
- Obesity, Physical Activity, Diet
- Animal Disease Management and Epidemiology
- Virus-based gene therapy research
University of Glasgow
2020-2025
Newcastle University
2014-2022
Newcastle Hospitals - Campus for Ageing and Vitality
2015-2022
Universitat de Lleida
2020
Biomedical Research Institute of Lleida
2020
Universidad Autónoma de Madrid
2015-2018
Inmunal (Spain)
2012-2016
University of Newcastle Australia
2015
Tampere University
2008-2015
Tampere University Hospital
2009-2013
Increased production of reactive oxygen species (ROS) has long been considered a cause aging. However, recent studies have implicated ROS as essential secondary messengers. Here we show that the site significantly contributes to their apparent dual nature. We report increase with age mitochondrial function deteriorates. also demonstrate increasing specifically through respiratory complex I reverse electron transport extends Drosophila lifespan. Reverse rescued pathogenesis induced by severe...
Cellular homoeostatic pathways such as macroautophagy (hereinafter autophagy) are regulated by basic mechanisms that conserved throughout the eukaryotic kingdom. However, it remains poorly understood how these further evolved in higher organisms. Here we describe a modification autophagy pathway vertebrates, which promotes its activity response to oxidative stress. We have identified two oxidation-sensitive cysteine residues prototypic receptor SQSTM1/p62, allow activation of pro-survival...
Inflammation is a hallmark of aging and negatively affecting female fertility. In this study, we evaluate the role NLRP3 inflammasome in ovarian Age-dependent increased expression ovary was observed WT mice during reproductive aging. High NLRP3, caspase-1, IL-1β also granulosa cells from patients with insufficiency. Ablation improved survival pregnancy rates anti-Müllerian hormone levels autophagy ovaries. Deficiency reduced serum FSH estradiol levels. Consistent these results,...
SPECIFIC AIMSIn this investigation the effect of caloric restriction (CR), only experimental manipulation that decreases aging rate, on mitochondrial reactive oxygen species (ROS) generation an...
Background Aging results in a progressive loss of skeletal muscle, condition known as sarcopenia. Mitochondrial DNA (mtDNA) mutations accumulate with aging muscle and correlate loss, although no causal relationship has been established. Methodology/Principal Findings We investigated the between mtDNA sarcopenia at gene expression biochemical levels using mouse model that expresses proofreading-deficient version (D257A) mitochondrial Polymerase γ, resulting increased spontaneous mutation...
Previous studies have consistently shown that caloric restriction (CR) decreases mitochondrial reactive oxygen species (ROS) (mitROS) generation and oxidative damage to mtDNA proteins, increases maximum longevity, although the mechanisms responsible for this are unknown. We recently found protein (PR) also produces these changes independent of energy restriction. Various facts link methionine aging, (MetR) without increases, like CR, longevity. thus hypothesized MetR is decrease in mitROS...
The Mitochondrial Free Radical Theory of Aging (MFRTA) proposes that mitochondrial free radicals, produced as by-products during normal metabolism, cause oxidative damage. According to MFRTA, the accumulation this damage is main driving force in aging process. Although widely accepted, theory remains unproven, because evidence supporting it largely correlative. For example, long-lived animals produce fewer radicals and have lower levels their tissues. However, does not prove radical...
Mutations in mitochondrial oxidative phosphorylation complex I are associated with multiple pathologies, and has been proposed as a crucial regulator of animal longevity. In yeast, the single-subunit NADH dehydrogenase Ndi1 serves non-proton-translocating alternative enzyme that replaces I, bringing about reoxidation intramitochondrial NADH. We have created transgenic strains Drosophila express yeast NDI1 ubiquitously. Mitochondrial extracts from NDI1-expressing flies displayed...
Abstract The helicase Twinkle is indispensable for mtDNA replication in nucleoids. Previously, we showed that tightly membrane-associated even the absence of mtDNA, which suggests part a membrane-attached platform. Here show this platform cholesterol-rich membrane structure. We fractionated mitochondrial preparations on flotation gradients and nucleoids accumulate at top gradient. This fraction was shown to be highly enriched cholesterol, lipid otherwise low abundant mitochondria. In...
Autophagy is an essential catabolic process that promotes the clearance of surplus or damaged intracellular components. Loss autophagy in age-related human pathologies contributes to tissue degeneration through a poorly understood mechanism. Here, we identify evolutionarily conserved role from yeast humans preservation nicotinamide adenine dinucleotide (NAD) levels, which are critical for cell survival. In respiring mouse fibroblasts with deficiency, loss mitochondrial quality control was...
Eleven monoclonal antibodies specific to plum pox potyvirus (PPV) coat protein were obtained by hybridoma technology from Spanish PPV isolates. In addition, two for cylindrical inclusions (CIP non‐structural proteins) obtained. The assayed DASI ELISA against 81 At least nine different epitopes found and 21 distinct serological patterns of reaction (serogroups) established using selected the collection isolates, indicating high variability among Changes in epitope composition observed after...
Summary Monoclonal antibodies (MAb) specific for citrus tristeza virus (CTV) were obtained from hybrid cells produced by fusion of a non-secreting myeloma cell line with spleen BALB/c mice immunized isolate T-308 CTV. Three MAb characterized their immunoglobulin isotype and titres in culture ascites fluids. Each was conjugated alkaline phosphatase used double-antibody sandwich enzyme-linked immunosorbent assay When tested against 23 strains CTV, each recognized all the uniform reactions low...
The Mitochondrial Free Radical Theory of Aging (MFRTA) is currently one the most widely accepted theories used to explain aging. From MFRTA three basic predictions can be made: long-lived individuals or species should produce fewer mitochondrial Reactive Oxygen Species (mtROS) than short-lived species; a decrease in mtROS production will increase lifespan; and an lifespan. It possible add further fourth prediction: if ROS controlling longevity separating these parameters through selection...
Caloric restriction (CR) decreases oxidative damage, which contributes to the slowing of aging rate. It is not known if such are due calories themselves or specific dietary components. In this work, ingestion proteins Wistar rats was decreased by 40% below that controls. After 7 weeks, liver protein-restricted (PR) animals showed in protein degree membrane unsaturation, and mitochondrial complex I content. The results previous information suggest decrease rate induced PR can be part reactive...