A5052762271- Mitochondrial Function and Pathology
- Genetics, Aging, and Longevity in Model Organisms
- Invertebrate Immune Response Mechanisms
- Insect symbiosis and bacterial influences
- Neurobiology and Insect Physiology Research
- ATP Synthase and ATPases Research
- Endoplasmic Reticulum Stress and Disease
- Physiological and biochemical adaptations
- Mosquito-borne diseases and control
- Insect Resistance and Genetics
- Ubiquitin and proteasome pathways
- Diet and metabolism studies
- Phytoplasmas and Hemiptera pathogens
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Autophagy in Disease and Therapy
- Insect and Pesticide Research
- Birth, Development, and Health
- Antimicrobial Peptides and Activities
- Aquaculture disease management and microbiota
- Coenzyme Q10 studies and effects
- interferon and immune responses
- Advanced Glycation End Products research
- Immune responses and vaccinations
Tampere University
2010-2024
Tampere University Hospital
2009-2019
Mutations in mitochondrial oxidative phosphorylation complex I are associated with multiple pathologies, and has been proposed as a crucial regulator of animal longevity. In yeast, the single-subunit NADH dehydrogenase Ndi1 serves non-proton-translocating alternative enzyme that replaces I, bringing about reoxidation intramitochondrial NADH. We have created transgenic strains Drosophila express yeast NDI1 ubiquitously. Mitochondrial extracts from NDI1-expressing flies displayed...
Mitochondrial dysfunction is a significant factor in human disease, ranging from systemic disorders of childhood to cardiomyopathy, ischaemia and neurodegeneration. Cytochrome oxidase, the terminal enzyme mitochondrial respiratory chain, frequent target. Lower eukaryotes possess alternative respiratory-chain enzymes that provide non-proton-translocating bypasses for complexes I (single-subunit reduced nicotinamide adenine dinucleotide dehydrogenases, e.g. Ndi1 yeast) or III + IV [alternative...
Abstract Background Mitochondria participate in various cellular processes including energy metabolism, apoptosis, autophagy, production of reactive oxygen species, stress responses, inflammation and immunity. However, the role mitochondrial metabolism immune cells tissues shaping innate responses are not yet fully understood. We investigated effects tissue-specific perturbation on at organismal level. Genes for oxidative phosphorylation (OXPHOS) complexes cI-cV were knocked down fruit fly...
The Drosophila mutant tko25t exhibits a deficiency of mitochondrial protein synthesis, leading to global insufficiency respiration and oxidative phosphorylation. This entrains an organismal phenotype developmental delay sensitivity seizures induced by mechanical stress. We found that the is exacerbated in dose-dependent fashion high dietary sugar levels. larvae were exhibit severe metabolic abnormalities further accentuated high-sugar diet. These include elevated pyruvate lactate, decreased...
To combat infection, an immune system needs to be promptly activated but tightly controlled avoid destructive effects on host tissues. IbinA and IbinB are related short peptides with robust expression upon microbial challenge in Drosophila melanogaster . Here, we show that Ibin genes ubiquitously present flies of the subgenus Sophophora , where they replace a different probably gene, Mibin which is found across much wider range cyclorrhaphan flies. Using synthetic peptides, did not observe...
Abstract A point mutation (stress-sensitive B1, sesB1 in the Drosophila gene coding for major adult isoform of adenine nuclear translocase (ANT) represents a model human diseases associated with ANT insufficiency. We characterized organismal, bioenergetic and molecular phenotype sesB1, then tested strategies to compensate mutant phenotype. In addition developmental delay bang-sensitivity, manifests impaired response sound, defective male courtship, female sterility curtailed lifespan. These...
An assembled cDNA coding for the putative single-subunit NADH dehydrogenase (NDX) of Ciona intestinalis was introduced into Drosophila melanogaster. The encoded protein found to localize mitochondria and confer rotenone-insensitive substrate oxidation in organello. Transgenic flies exhibited increased resistance menadione, starvation temperature stress, manifested a sex diet-dependent increase mean lifespan 20-50%. However, NDX able only weakly complement phenotypes produced by knockdown...
RNA 3′ polyadenylation is known to serve diverse purposes in biology, particular, regulating mRNA stability and translation. Here we determined that, upon exposure high levels of the intercalating agent ethidium bromide (EtBr), greater than those required suppress mitochondrial transcription, tRNAs human cells became polyadenylated. Relaxation inducing stress led rapid turnover polyadenylated tRNAs. The extent, kinetics duration tRNA were EtBr dose-dependent, with differentially sensitive...
Abstract The mitochondrial alternative oxidase, AOX, present in most eukaryotes apart from vertebrates and insects, catalyzes the direct oxidation of ubiquinol by oxygen, by‐passing terminal proton‐motive steps respiratory chain. Its physiological role is not fully understood, but it proposed to buffer stresses chain similar those encountered diseases humans. Previously, we found that ubiquitous expression AOX Ciona intestinalis Drosophila perturbs development flies cultured under...
Carbonic anhydrases (CAs, EC 4.2.1.1) are ubiquitous enzymes that catalyze the reversible hydration reaction of carbon dioxide. CAs present as six structurally divergent enzyme families: α, β, γ, δ, ζ and η. β-CAs have a wide distribution across different species including invertebrates. Previously, we showed Drosophila melanogaster β-CA is highly active mitochondrial enzyme. In this study, investigated function by silencing expression gene using UAS/GAL4-based RNA interference (RNAi) in...
The Drosophila bang-sensitive mutant tko25t, manifesting a global deficiency in oxidative phosphorylation due to mitochondrial protein synthesis defect, exhibits pronounced delay larval development. We previously identified number of metabolic abnormalities tko25t larvae, including elevated pyruvate and lactate, found the gut be crucial tissue for regulation growth mutant. Here we established that expression wild-type tko any several other tissues also partially alleviates developmental...
Abstract The role of mitochondria in both adaptive and innate immune responses is increasingly recognized, but the mitochondrial DNA (mtDNA) variation as an immunomodulatory factor has received less attention. One reason for this difficulty separating effect mtDNA from that nuclear genome. By utilizing fruit fly Drosophila melanogaster , a powerful model system, we created cytoplasmic hybrids, aka. cybrid lines, where unique mtDNAs (mitotypes) were introgressed into controlled isogenic...
The role of mitochondria in immunity is increasingly recognized, but it unclear how variation mitochondrial DNA (mtDNA) contributes to variable infection outcomes. To quantify the effect mtDNA on humoral and cell-mediated innate immune responses, we utilized a panel fruit fly Drosophila melanogaster cytoplasmic hybrids (cybrids), where unique mtDNAs (mitotypes) were introgressed into controlled isogenic nuclear background. We observed substantial heterogeneity outcomes within cybrid upon...
Withdrawal Statement The authors have withdrawn version 2 of this manuscript due to the erroneous revision with a related but different manuscript, which can now be found under doi: 10.1101/2024.03.28.587162. Note that 1 preprint is correct and has been published as 10.1186/s12915-024-01858-5, should used for citation purposes. If you any questions, please contact corresponding author (Tiina S. Salminen, tiina.s.salminen@tuni.fi ).
ABSTRACT The Drosophila tko 25t point mutation in the gene encoding mitoribosomal protein S12 produces a complex phenotype of multiple respiratory chain deficiency, developmental delay, bang-sensitivity, impaired hearing, sugar and antibiotic sensitivity male courtship. Its phenotypic severity was previously shown to be alleviated by inbreeding, vary with mitochondrial genetic background. Here we show similarly profound effects conferred nuclear We backcrossed into each two standard...
Abstract The Drosophila tko25t point mutation in the gene encoding mitoribosomal protein S12 produces a complex phenotype of multiple respiratory chain deficiency, developmental delay, bang-sensitivity, impaired hearing, sugar and antibiotic sensitivity, male courtship. Its phenotypic severity was previously shown to be alleviated by inbreeding vary with mitochondrial genetic background. Here, we show similarly profound effects conferred nuclear We backcrossed into each 2 standard...