A5067986637- Neurobiology and Insect Physiology Research
- Genetics, Aging, and Longevity in Model Organisms
- Mitochondrial Function and Pathology
- Microtubule and mitosis dynamics
- Cholinesterase and Neurodegenerative Diseases
- Genetic Neurodegenerative Diseases
- Cellular Mechanics and Interactions
- ATP Synthase and ATPases Research
- Zebrafish Biomedical Research Applications
- Invertebrate Immune Response Mechanisms
- Plant Molecular Biology Research
- Hereditary Neurological Disorders
- Photosynthetic Processes and Mechanisms
- Birth, Development, and Health
- Adipose Tissue and Metabolism
Tampere University
2015-2020
University of Helsinki
2019-2020
RIKEN Center for Brain Science
2011-2015
Tampere University Hospital
2015
Saitama University
2010-2011
Abstract The mitochondrial alternative oxidase, AOX, carries out the non proton-motive re-oxidation of ubiquinol by oxygen in lower eukaryotes, plants and some animals. Here we created a modified version AOX from Ciona instestinalis , carrying mutations at conserved residues predicted to be required for chelation diiron prosthetic group. protein was stably expressed mammalian cells or flies, but lacked enzymatic activity unable rescue phenotypes flies knocked down subunit cytochrome oxidase....
: Huntington's disease is an autosomal dominant neurodegenerative disorder that caused by abnormal expansion of a polyglutamine tract in the huntingtin protein, resulting intracellular aggregate formation and neurodegeneration. How neuronal cells are affected such remains obscure. To dissect ways which can cause neural dysfunction, authors generated Drosophila transgenic strains expressing either nuclear targeted or cytoplasmic form pathogenic (NHtt-152QNLS, NHtt-152Q), nonpathogenic...
To understand how differences in complex cell shapes are achieved, it is important to accurately follow microtubule organization. The Drosophila larval body wall contains several types that models study and tissue morphogenesis. For example tracheae used examine tube morphogenesis1, the dendritic arborization (DA) sensory neurons of larva have become a primary system for elucidation general neuron-class-specific mechanisms differentiation2-5 degeneration6. shape dendrite branches can vary...
Mitochondria have been increasingly recognized as a central regulatory nexus for multiple metabolic pathways, in addition to ATP production via oxidative phosphorylation (OXPHOS). Here we show that inducing mitochondrial DNA (mtDNA) stress Drosophila using mitochondrially-targeted Type I restriction endonuclease (mtEcoBI) results unexpected reprogramming adult flies, distinct from effects on OXPHOS. Carbohydrate utilization was repressed, with catabolism shifted towards lipid oxidation,...
The Drosophila bang-sensitive mutant tko25t, manifesting a global deficiency in oxidative phosphorylation due to mitochondrial protein synthesis defect, exhibits pronounced delay larval development. We previously identified number of metabolic abnormalities tko25t larvae, including elevated pyruvate and lactate, found the gut be crucial tissue for regulation growth mutant. Here we established that expression wild-type tko any several other tissues also partially alleviates developmental...
Dopaminergic (DA) neurons have been implicated as key targets in neurological disorders, notably those involving locomotor impairment, and are considered to be highly vulnerable mitochondrial dysfunction, a common feature of such diseases. Here we investigated Drosophila model disorders which functional impairment is brought about by pan-neuronal RNAi knockdown subunit COX7A cytochrome oxidase (COX). Despite minimal neuronal loss apoptosis, the expression activity tyrosine hydroxylase was...
To understand how differences in complex cell shapes are achieved, it is important to accurately follow microtubule organization. The Drosophila larval body wall contains several types that models study and tissue morphogenesis. For example tracheae used examine tube morphogenesis1, the dendritic arborization (DA) sensory neurons of larva have become a primary system for elucidation general neuron-class-specific mechanisms differentiation2-5 degeneration6. shape dendrite branches can vary...
Dopaminergic (DA) neurons have been implicated as key targets in Parkinson's disease (PD) and other neurological disorders, are considered to be highly vulnerable mitochondrial dysfunction, a common feature of such diseases. Here we investigated Drosophila model PD which locomotor impairment is brought about by pan-neuronal RNAi knockdown subunit COX7A cytochrome oxidase (COX). Despite minimal neuronal loss apoptosis, the expression activity tyrosine hydroxylase was decreased half....