A5037759954- Genetics, Aging, and Longevity in Model Organisms
- Neurobiology and Insect Physiology Research
- Insect Utilization and Effects
- Invertebrate Immune Response Mechanisms
- Aquaculture Nutrition and Growth
- Circadian rhythm and melatonin
- Cholinesterase and Neurodegenerative Diseases
- Physiological and biochemical adaptations
- Medicinal Plants and Bioactive Compounds
- Lipid metabolism and biosynthesis
- Pharmacological Effects of Natural Compounds
- Medicinal Plants and Neuroprotection
- Curcumin's Biomedical Applications
- Muscle Physiology and Disorders
- Olfactory and Sensory Function Studies
- Muscle metabolism and nutrition
- Exercise and Physiological Responses
- Complementary and Alternative Medicine Studies
- Diet, Metabolism, and Disease
- Dermatological and COVID-19 studies
- Diet and metabolism studies
- Animal Behavior and Reproduction
- Genetics and Physical Performance
- COVID-19 Clinical Research Studies
- Agriculture and Biological Studies
Vasyl Stefanyk Precarpathian National University
2011-2024
Fruit flies have eight identified Drosophila insulin-like peptides (DILPs) that are involved in the regulation of carbohydrate concentrations hemolymph as well accumulation storage metabolites. In present study, we investigated diet-dependent roles DILPs encoded by genes dilp1-5, and dilp7 insect appetite, food choice, triglycerides, glycogen, glucose, trehalose fruit fly bodies carbohydrates hemolymph. We found wild type mutant lines demonstrate compensatory feeding for carbohydrates....
It has recently been demonstrated that as the ratio of protein to carbohydrate (P:C) in diet declines, life span increases Drosophila. Here we explored how extremely low dietary ratios affected longevity and a selection variables associated with functional senescence. An increase P:C from 1:57 1:20 shortened by increasing age-dependent mortality; whereas further decline 1:95 caused modest decrease span. Female flies consuming 1:38 diets laid more eggs than those lower diets. Flies fed higher...
The fruit fly, Drosophila melanogaster is a broadly used model for gerontological research. Many studies are dedicated to understanding nutritional effects on ageing; however, the influence of dietary carbohydrate type and dosage still poorly understood. We show that among three carbohydrates tested, fructose, glucose, sucrose, latter decreased life span by 13%–27%, being present in concentrations 2%–20% diet. Life-span shortening sucrose was accompanied an increase age-independent...
Abstract Macronutrient intake impacts physiology, behavior, and gene expression in a wide range of organisms. We used the response surface methodology to compare how life history traits, lifespan, reproduction differ as function protein carbohydrate intakes under choice no-choice feeding regimens fruit fly, Drosophila melanogaster. found that when offered nutritionally complementary foods mated female flies regulated toward ratio (P:C) was associated with shortened lifespan maximal egg...
This study was conducted to evaluate the effects of rhizome powder from herb Rhodiola rosea, a traditional Western Ukraine medicinal adaptogen, on lifespan and age-related physiological functions fruit fly Drosophila melanogaster.Flies fed food supplemented with 5.0 mg/ml 10.0 R. rosea had 14% 17% higher median lifespan, whereas at 30.0 decreased by 9% 12%. The preparation did not decrease fecundity.The effect supplement dependent diet composition. Lifespan extension 15% 21% observed only...
The impact of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) on lung tissue in patients support is significant scientific interest predicting mortality. This study aimed to analyze post-mortem histological changes COVID-19 using vital radiology semiotics. A total 41 autopsies were performed who died SARS-CoV-2 and had confirmed by polymerase chain reaction (PCR) radiological evidence consolidation ground glass opacity. results showed that duration was significantly...
Mitochondrial alternative NADH dehydrogenase (aNDH) was found to extend lifespan when expressed in the fruit fly. We have that flies expressing aNDH from Ciona intestinalis (NDX) had 17-71% prolongation on media with different protein-tocarbohydrate ratios except NDX-expressing males 19% shorter than controls a high protein diet. were more resistant organic xenobiotics, 2,4-dichlorophenoxyacetic acid and alloxan, inorganic toxicant potassium iodate, partially sodium molybdate treatments. On...
The insulin–IGF-1 signaling (IIS) pathway is conserved throughout multicellular organisms and regulates many traits, including aging, reproduction, feeding, metabolism, stress resistance, growth. Here, we present evidence of a survival-sustaining role for IIS in subset gut cells Drosophila melanogaster, namely the intestinal stem (ISCs) progenitor cells. Using RNAi to knockdown insulin receptor, found that inhibition ISCs statistically shortened lifespan experimental flies compared with...
Aging is often associated with accumulation of oxidative damage in proteins and lipids. However, some studies do not support this view, raising the question whether high levels are lifespan. In current investigation, Drosophila melanogaster flies were kept on diets 2 or 10% either glucose fructose. The lifespan, fecundity, feeding as well amounts protein carbonyls (PC) lipid peroxides (LOOH), activities superoxide dismutase (SOD), catalase, glutathione-S-transferase (GST), glutathione...
Abstract Interventions such as caloric or dietary restriction extend lifespan in organisms spanning from yeast to primates. Despite its positive influence on longevity, has been found negatively affect reproduction. Many studies have reported negative correlations between and reproductive characteristics (such mating rate, fecundity, period, others). Such correlation gives the appearance of a resource‐based trade‐off these two life‐history traits. Here, we used nutritional geometry confirm...
Aging is a multifactorial process which affects all animals. as result of damage accumulation the most accepted explanation but proximal causes remain to be elucidated. There also evidence indicating that aging has an important genetic component. Animal species age at different rates and specific signaling pathways, such insulin/insulin-like growth factor, can regulate life span individuals within by reprogramming cells in response environmental changes. Here, we use unbiased approach...
Background. Nutrition during growth and development affects various traits not only in larvae but also imago including lifespan, reproduction, feeding, metabolism, stress resistance. In this study, we have tested the hypothesis of whether dietary protein-to-carbohydrate (P:C) ratio developmental diet could be related to subsequent changes metabolic profile physiological parameters Drosophila imago. Materials Methods. melanogaster Canton-S strain were used study. Larvae fed diets with...
<title>Abstract</title> Uncouplers of mitochondrial electron transport chain, such as 2,4-dinitrophehol (DNP), can mimic calorie restriction by decreasing efficiency adenosine triphosphate (ATP) synthesis. However, DNP is also a toxic substance, whose overdosage be lethal. In the fruit fly, <italic>Drosophila melanogaster</italic> model, we have found that in concentrations 0.05–0.2 g/L, led to drastic decrease fly survival on low caloric diet (1% sucrose and 1% yeast; 1S-1Y). On 5S-5Y diet,...
In a few last decades oxidative stress detected in variety of physiological processeswhere reactive oxygen species (ROS) and nitrogen (RNS) play central role. Theyare directly involved oxidation proteins, lipids nucleic acids. certain concentrationsthey are necessary for cell division, proliferation apoptosis. Contractile muscle tissue at aerobicconditions form high ROS flow that may modulate functions, exampleproliferation. However, slight increase level provide hormetic effect which...
SUMMARY Fruit flies have eight identified Drosophila insulin-like peptides (DILPs) involved in regulation of carbohydrate concentrations hemolymph as well accumulation storage metabolites. In the present study, we investigated diet-dependent roles DILPs encoded by genes dilp1–5 , and dilp7 insect appetite, food choice, triglycerides, glycogen, glucose, trehalose fruit fly body carbohydrates hemolymph. We found that dilp2 gene predominantly influences glycogen level, dilp3 – level hemolymph,...