A new symptomatology was observed in celery (Apium graveolens) Villena, Spain 2008. Symptomatology included an abnormal amount of shoots per plant and curled stems. These vegetative disorders were associated with 'Candidatus Liberibacter solanacearum' not phytoplasmas. Samples from sap immobilized on membranes based the spot procedure tested using a newly developed real-time polymerase chain reaction assay to detect 'Ca. L. solanacearum'. Then, test kit validated by intralaboratory assays...

In 2009 and 2010, commercial carrot (Daucus carota L.) fields located in Tenerife (Canary Islands, Spain) showed symptoms of curling, yellow, bronze, purple discoloration leaves, stunting shoots tap roots, proliferation secondary roots. A large population the psyllid Bactericera trigonica was noted those fields. Similar were reported previously carrot-production areas Canary Islands mainland Spain that associated with stolbur aster yellows (1997 1998) (2) Spiroplasma citri phytoplasmas (2009...

Tomato brown rugose fruit virus (ToBRFV) is a highly infectious tobamovirus that causes severe disease in tomato (Solanum lycopersicum L.) crops. In Italy, the first ToBRFV outbreak occurred 2018 several provinces of Sicily region. represents serious threat for crops Italy and Mediterranean Basin.Molecular biological characterisation Sicilian ToB-SIC01/19 isolate was performed, sensitive specific Real-time RT-PCR TaqMan minor groove binder probe method developed to detect infected plants...

In the summer of 2008, symptoms leaf curling with yellow, bronze, and purple discoloration, twisting petioles, stunting shoots tap roots, proliferation secondary roots were observed in 18 commercial carrot (Daucus carota L.) fields (~62 ha) severely infested psyllids (mainly Bactericera sp.) from 52 (297 located Alicante Albacete provinces Spain. Incidence symptomatic plants was variable among fields. Similar 2009, 2010, 2011. Symptoms resembled those associated phytoplasma, spiroplasma, or...

In October 2019, tomato (Solanum lycopersicum L.) plants showing chlorosis and brown necrosis in apical leaflets rugose surface fruits were observed a greenhouse Vicar, Almería, Spain. A total of 0.5% the (1,38 ha) showed these symptoms. The presence fruit virus (ToBRFV) was suspected. 5 symptomatic 2 symptomless leaf samples collected analyzed by double-antibody sandwich (DAS)-ELISA with antibodies for ToBRFV, tobacco mosaic (TMV) (ToMV) (Loewe Biochemica, Germany). Symptomatic tested...

Rates of seed transmission for Pepino mosaic virus (PepMV) were estimated in seedlings grown from seeds obtained symptomatic tomato (Lycopersicon esculentum) fruits plants naturally infected with the virus. The proportion PepMV was at least 25% as enzyme-linked immunosorbent assay (ELISA) analysis grouped seeds. planted, and cotyledon transplant stage assayed by ELISA. Three 168 PepMV-positive, corresponding to a seed-to-seedling rate 1.84%. Various treatments evaluated their ability prevent...

Transmission of Pepino mosaic virus (PepMV) by the fungal vector Olpidium virulentus was studied in two experiments. Two characterized cultures fungus were used as stock for assay: culture A from lettuce roots collected Castellón (Spain), and B tomato Murcia (Spain). These maintained their original host irrigated with sterile water. The drainage water irrigating these watering PepMV-infected non-infected plants to constitute acquisition–source assay, which divided into six different plots:...

Pepino mosaic virus (PepMV) has caused great concern in the greenhouse tomato industry after it was found causing a new disease 1999. The objective of this paper is to investigate alternative hosts and compare important biological characteristics three PepMV strains occurring Europe when tested under different environmental conditions. To end we compared infectivity symptom development three, well characterized isolates belonging strains, EU-tom, Ch2 US1, by inoculating them on tomato,...

In February of 2008, in open-field-grown tomato crops (Solanum lycopersicum L.) from the central regions Coclé, Herrera, Los Santos, and Veraguas Panama, unusual disease symptoms, including deformation, necrosis, purple margins, interveinal yellowing, downward upward curling leaflets alternately, necrotic lines sepals branches, fruits distorted with on surface, severe stunting, were observed. Tomato production was seriously damaged. To verify identity disease, five symptomatic plants four...

In November 2012, unusual symptoms were observed in plants of sweet pepper (Capsicum annuum L.) grown commercial greenhouses Almería Province, southeastern Spain. Symptoms included interveinal yellowing, upward leaf curling, and internode shortening, more evident the upper part plant. Abnormal ripening fruits was symptomatic plants, with remaining orange red varieties yellow varieties, thus reducing their marketability. During December 2012 January 2013, severe outbreaks this disease...

Abstract Southern tomato virus (STV) is a double‐stranded RNA (dsRNA) belonging to the genus Amalgavirus from family Amalgamaviridae . STV has been detected in plants showing symptoms of stunting, fruit discoloration and size reduction, although its role on symptom development unclear. Also, little known about incidence epidemiology this how it spreads crops. In work, we developed molecular hybridisation method by using digoxigenin‐labelled probe based nucleotide sequence putative coat...

The genetic variation and population structure of gene N (nucleocapsid) part L (replicase) from 13 eggplant mottle dwarf virus (EMDV) isolates Spain were evaluated compared with sequences EMDV other countries retrieved GenBank. Phylogenetic inference showed three main clades, one containing an isolate Australia the two Iran Europe, as well four subclades. genetically very similar grouped in a subclade together Germany UK. No new recombination events detected addition to previously reported,...

Rates of seed transmission Melon necrotic spot virus (MNSV) were estimated in seedlings grown from commercial melon ( Cucumis melo ) cv. Galia F 1 seeds. Seedlings at the cotyledon stage and adult plants assayed for MNSV by DAS‐ELISA RT‐PCR. None seedling groups tested positive ELISA. The proportion infected with was least 7 8% lots 05 06, respectively, as RT‐PCR analysis grouped seedlings. Fourteen eight samples (10 per group), a total 200 100 seedlings, seeds MNSV‐positive corresponding to...

Abstract In surveys to determine the occurrence and distribution of ‘torrado’ disease (Tomato torrado virus, ToTV) in main Spanish tomato growing areas from 2001 2008, a total 584 samples symptomatic asymptomatic plants were collected 92 greenhouses. The tests showed that 451 85 greenhouses different infected with ToTV. majority positive typical symptoms disease. However, showing necrosis even virus. Co‐infection ToTV Pepino mosaic virus (PepMV) occurred large number (60.5%), several other...

Tomato torrado virus (ToTV) is a recently identified Picorna-like that causes "torrado disease" in tomatoes (4). Typical symptoms of seen tomato crops (Solanum lycopersicum L. formerly Lycopersicon esculentum L.) were initially defined as yellow areas at the base leaflet later developed into necrotic spots sometimes abscised, leaving holes leaflet. Other plants showed extensive necrosis progressing from to tip Fruits distorted with lines on surface often cracked. Affected had burnt-like...

During the growing seasons of 2007 and 2008, in commercial greenhouses tomato crops (Solanum lycopersicum L.) located Szeged, Öcsöd, Csongrád (southeastern regions Hungary), unusual disease symptoms were observed, including necrotic spots defined areas at base leaflet, necrosis stems, lines on fruits surface. Affected plants appeared inside with a random distribution incidence recorded was least 40%. These resembled those described for Tomato torrado virus (ToTV) infection Spain (1) Poland...

In 2003, greenhouse-grown tomato crops (Lycopersicon esculentum Mill.) in the Canary Islands (Spain) were observed showing an initial yellowing defined areas at base of leaflet that later developed into necrotic spots or extensive area progressing from to tip. Fruits also affected, and often developing cracking. Generally, plants affected seemed be burnt, their growth was reduced, production level seriously damaged. Similar symptoms have been Murcia since 2001, which recently associated with...

In 2008 and 2009, symptoms of curling, yellow purple discoloration leaves, stunting shoots tap roots, formation bunchy, fibrous secondary roots were observed in commercial carrot (Daucus carota L.) fields located several production areas Spain (Alicante, Albacete, Segovia, Valladolid). Incidence this disease was almost 100% individual affected fields. Similar reported from 1997 to 1998 various (the Canary Islands, Madrid) associated with infection stolbur aster yellows phytoplasmas (2)....