A5020883086- Plant Virus Research Studies
- Insect-Plant Interactions and Control
- Plant Pathogenic Bacteria Studies
- Plant and Fungal Interactions Research
- Plant-Microbe Interactions and Immunity
- Phytoplasmas and Hemiptera pathogens
- Plant Pathogens and Fungal Diseases
- Plant Disease Resistance and Genetics
- Plant Parasitism and Resistance
- Insect symbiosis and bacterial influences
- Plant tissue culture and regeneration
- COVID-19 Clinical Research Studies
- Cocoa and Sweet Potato Agronomy
- Biosensors and Analytical Detection
- Plant Physiology and Cultivation Studies
- Legume Nitrogen Fixing Symbiosis
- Postharvest Quality and Shelf Life Management
- Insect and Pesticide Research
- Animal Virus Infections Studies
- Entomopathogenic Microorganisms in Pest Control
- COVID-19 diagnosis using AI
- Mosquito-borne diseases and control
- Ultrasound in Clinical Applications
- Berry genetics and cultivation research
- Plant Pathogens and Resistance
University of Palermo
2015-2024
Ospedale Santa Maria Goretti
2020-2021
Istituto Euro Mediterraneo di Scienza e Tecnologia
2013-2018
Organizzazione per la Tutela Forestale, Ambientale e Agroalimentare
2010-2017
University of Catania
2015-2016
Tomato brown rugose fruit virus (ToBRFV) is a highly infectious virus, that becoming threat to tomato production worldwide. In this work we evaluated the localization of ToBRFV particles in seeds, its seed transmission rate and efficacy disinfection, effects different thermal- chemical-based treatments on ToBRFV-infected seeds’ germination. Analyses demonstrated was located coat, sometime endosperm, but never embryo; from infected seeds plantlets occurs by micro-lesions during The 2.8%...
In October 2018, virus-like symptoms were observed in four di erent greenhouses of tomato (Solanum lycopersicum) Ragusa province (Sicily, Italy).Symptoms consisted mosaic, deformation, and necrosis on young leaves, discoloration deformations fruits.In total 40 symptomatic samples collected (10 for each greenhouse).Samples tested by reverse transcription polymerase chain reaction (RT-PCR) using speci c primers viruses that incite similar plants:
Tomato leaf curl New Delhi virus (ToLCNDV) is a bipartite begomovirus (family Geminiviridae) which infects species in the families Cucurbitaceae and Solanaceae (Padidam et al., 4; Mizutani 3). Begomoviruses are transmitted by whitefly Bemisia tabaci persistent manner (Rosen 5). In October 2015, severe symptoms not previously reported growers horticultural area of Province Trapani (Sicily, Italy) were observed on zucchini squash (Cucurbita pepo) open fields. The included yellow mosaic,...
Tomato brown rugose fruit virus (ToBRFV) is a highly infectious tobamovirus that causes severe disease in tomato (Solanum lycopersicum L.) crops. In Italy, the first ToBRFV outbreak occurred 2018 several provinces of Sicily region. represents serious threat for crops Italy and Mediterranean Basin.Molecular biological characterisation Sicilian ToB-SIC01/19 isolate was performed, sensitive specific Real-time RT-PCR TaqMan minor groove binder probe method developed to detect infected plants...
Abstract Tomato ( Solanum lycopersicum L., family Solanaceae ) represents one of the most cultivated horticultural crops worldwide, with over 5 million hectares area and more than 182 tons tomato produced globally. Nevertheless, monoculture conditions, intensive selection, domestication throughout last decades, international trade infected propagating material climate changes intensely favoured establishment many pathogens rapid spread new diseases, allowing organisms to establish in...
Abstract Tomato spotted wilt virus (TSWV) is considered one of the most threatening viruses worldwide for different economically important agricultural crops. In this scenario, it to perform an early detection by laboratory tests prevent TSWV spread. A rapid and sensitive protocol based on real time reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed in work, also using cost-effective simplified sample preparation procedure, assess suitability RT-LAMP...
Tomato brown rugose fruit virus (ToBRFV) is an emerging pathogen that causes severe disease in tomato (Solanum lycopersicum L.) crops. The first ToBRFV outbreak Italy occurred 2018 several Sicilian provinces, representing a serious threat for production. In the present work, spatiotemporal displacement of Sicily was evaluated, analyzing total 590 lots seed, 982 plantlets from nurseries and 100 commercial greenhouses. Furthermore, we investigated spreading dynamic greenhouse under...
Tomato leaf curl New Delhi virus (ToLCNDV) represents a threat to economically important horticultural crops. A real-time loop-mediated isothermal amplification (LAMP) assay for in-field ToLCNDV detection was developed, coupled rapid sample preparation method, and tested both in field laboratory conditions on zucchini squash, tomato, pepper samples. set of six LAMP primers designed specific ToCLNDV detection, targeting 218-nucleotide sequence within the AV1 gene. The sensitivity, specificity...
Tomato leaf curl New Delhi virus (To LCNDV ) is a bipartite begomovirus (family Geminiviridae identified for the first time in 1995 Asia, from where it spread into several countries of Mediterranean basin. To was found Spain 2012, and subsequently Tunisia Italy. The outbreak Italy occurred at end 2015 Trapani province (Sicily) on zucchini squashes. Then 2016, infected plants Campania, Lazio Sardinia regions, 2017 Calabria. This study addressed dispersion genetic diversity isolates A total...
In January 2020, about 85% of a red sweet pepper (Capsicum annuum) crop in greenhouse located Ragusa province (Sicily, Italy) showed virus-like symptoms. Symptoms consisted slight mosaic and discoloration young leaves, vein clearing on browning the stem with strong necrosis intersection secondary branches, partial vegetative apex marbling, distortion fruits (Fig. 1). It is important to note that 2019 same was cultivated tomato, which had been removed due extensive infection caused by Tomato...
Tomato yellow leaf curl Sardinia virus and have co-existed in Italian tomato crops since 2002 reached equilibrium, with plants hosting molecules of both species plus their recombinants being the most frequent case. Recombination events are studied field samples, as well experimental co-infections, when were detected early 45 days following inoculation. In conditions, recombination breakpoints essentially absent regions corresponding to ORFs V2, CP C4, whereas density was highest 3'-terminal...
Citrus tristeza virus (CTV) outbreaks were detected in Sicily island, Italy for the first time 2002. To gain insight into evolutionary forces driving emergence and phylogeography of these CTV populations, we determined analyzed nucleotide sequences p20 gene from 108 isolates collected 2002 to 2009. Bayesian phylogenetic analysis revealed that mild severe belonging five different clades (lineages) introduced Phylogeographic showed four lineages co-circulated main citrus growing area located...
A real-time loop-mediated isothermal amplification (LAMP) assay was developed for simple, rapid and efficient detection of the Olea europaea geminivirus (OEGV), a virus recently reported in different olive cultivation areas worldwide. preliminary screening by end-point PCR OEGV conducted to ascertain presence Sicily. set six LAMP primers, targeting 209-nucleotide sequence elapsing region encoding coat protein (AV1) gene OEGV, designed specific detection. The specificity, sensitivity,...
Pepino mosaic virus (Pep MV ) is a highly infectious potexvirus that causes severe disease in tomato ( Solanum lycopersicum crops worldwide. In Sicily, the first outbreak was detected single greenhouse 2005 and it promptly eradicated. However, 2008, large number of greenhouses were simultaneously affected, impossible to eradicate or control virus. This study addressed dispersion genetic diversity Pep isolates obtained from comparison with worldwide isolates, gain insight into factors...
Abstract Viruses cause significant damage in agricultural crops worldwide. Disease management requires sensitive and specific tools for virus detection identification. Also, techniques need to be rapid keep pace with the continuous emergence of new viral diseases. The genus Fabavirus is composed five viruses infecting many economically important This research describes development a procedure based on flow‐through hybridisation ( FTH ), which faster than as conventional tissue‐prints from...