A5006395432- Nanoparticle-Based Drug Delivery
- Nanoparticles: synthesis and applications
- Gold and Silver Nanoparticles Synthesis and Applications
- Nanoplatforms for cancer theranostics
- Advanced biosensing and bioanalysis techniques
- Quantum Dots Synthesis And Properties
- Metal-Organic Frameworks: Synthesis and Applications
- Advanced Nanomaterials in Catalysis
- Graphene and Nanomaterials Applications
- Polymer Surface Interaction Studies
- Characterization and Applications of Magnetic Nanoparticles
- Dendrimers and Hyperbranched Polymers
- Advanced Biosensing Techniques and Applications
- Biosensors and Analytical Detection
- Acute Ischemic Stroke Management
- Photoacoustic and Ultrasonic Imaging
- RNA Interference and Gene Delivery
- Nanocluster Synthesis and Applications
- Extracellular vesicles in disease
- Magnetic Properties and Synthesis of Ferrites
- Nanopore and Nanochannel Transport Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- 3D Printing in Biomedical Research
- Protein Interaction Studies and Fluorescence Analysis
- Luminescence and Fluorescent Materials
Center for Research in Molecular Medicine and Chronic Diseases
2016-2025
Universidade de Santiago de Compostela
2016-2025
Philipps University of Marburg
2012-2020
Centro Singular de Investigación en Química Biológica y Materiales Moleculares
2018
Universidad de Zaragoza
2009-2018
Instituto de Nanociencia y Materiales de Aragón
2009-2016
Universidad de Valladolid
2006-2007
Here we have investigated the effect of enshrouding polymer-coated nanoparticles (NPs) with polyethylene glycol (PEG) on adsorption proteins and uptake by cultured cells. PEG was covalently linked to polymer surface maximal grafting density achievable under our experimental conditions. Changes in effective hydrodynamic radius NPs upon human serum albumin (HSA) fibrinogen (FIB) were measured situ using fluorescence correlation spectroscopy. For without a shell, thickness increase around 3 nm,...
The protein adsorption layer (a.k.a. the “protein corona”) that forms on surface of colloidal nanoparticles plays an important role in their interaction with living matter. Thus, characterization corona is utmost importance for understanding how exposure to affects biological responses cells and organisms. Although a lot experimental studies have been reported this direction, comprehensive picture still missing, particular due multitude different scenarios under which experiments performed....
We report a procedure to prepare highly monodisperse copper telluride nanocubes, nanoplates, and nanorods. The is based on the reaction of salt with trioctylphosphine in presence lithium bis(trimethylsilyl)amide oleylamine. CuTe nanocrystals display strong near-infrared optical absorption associated localized surface plasmon resonances. exploit this resonance for design surface-enhanced Raman scattering sensors unconventional probes. Furthermore, we also here our preliminary analysis use as...
The photothermal response of plasmonic nanomaterials can be exploited for a number biomedical applications in diagnostics (biosensing and optoacoustic imaging) therapy (drug delivery therapy). most common cellular to cancer treatment (ablation solid tumors) using is necrosis, process that releases intracellular constituents into the extracellular milieu producing detrimental inflammatory responses. Here we report use laser-induced employing gold nanoprisms (NPRs) specifically induce...
Nanomaterials offer opportunities to construct novel compounds for many different fields. Applications include devices energy, including solar cells, batteries, and fuel health, contrast agents mediators photodynamic therapy hyperthermia. Despite these promising applications, any new class of materials also bears a potential risk human health the environment. The advantages innovations must be thoroughly compared against risks evaluate each nanomaterial. Although nanomaterials are often used...
Synthesis, characterization, and applications of colloidal nanoparticles have been a prominent topic current research interests within the last two decades. Available reports in literature that describe synthesis are abundant with various degrees reproducibility simplicity. Moreover, different methods for characterization nanoparticle's basic properties employed, resulting conflicting results many cases. Herein, we "in detail" selected standard protocols synthesis, purification, types...
Colloidal nanoparticles (NPs) have become versatile building blocks in a wide variety of fields. Here, we discuss the state-of-the-art, current hot topics, and future directions based on following aspects: narrow size-distribution NPs can exhibit protein-like properties; monodispersity is not always required; assembled collective behavior; be one by one; there more to connected with NPs; designed smart; surface-modified directly reach cytosols living cells.
Dual enzymatic reactions were introduced to fabricate programmed gemcitabine (GEM) nanovectors for targeted pancreatic cancer therapy. Dual-enzyme-sensitive GEM prepared by conjugation of matrix metalloproteinase-9 (MMP-9) detachable poly(ethylene glycol) (PEG), cathepsin B-cleavable GEM, and targeting ligand CycloRGD CdSe/ZnS quantum dots (QDs). The decorated with a PEG corona could avoid nonspecific interactions exhibit prolonged blood circulation time. After accumulated in tumor tissue...
The paper describes a novel and straightforward wet-chemical synthetic route to produce biocompatible single-crystalline gold tabular nanoparticles, herein called nanoprisms (NPRs) due their characteristic shape. Besides the novelty of method NPRs with an unprecedented high yield, synthesis avoids use highly toxic cetyltrimethylammonium bromide (CTAB), most widely used surfactant for anisotropic nanoparticles such as nanorods or nanoprisms. presented here allows tuning edge length in range...
The transport and the delivery of drugs through nanocarriers is a great challenge pharmacology. Since production liposomes to reduce toxicity doxorubicin in patients, plethora nanomaterials have been produced characterized. Although it widely known that elementary properties influence their vivo kinetics, such interaction often poorly investigated many preclinical studies. present study aims evaluate actual effect size shape on biodistribution set gold nanoparticles (GNPs) after intravenous...
Abstract Early detection of cancer greatly increases the chances a simpler and more effective treatment. Traditional imaging techniques are often limited by shallow penetration, low sensitivity, specificity, poor spatial resolution or use ionizing radiation. Hybrid modalities, like optoacoustic imaging, an emerging molecular modality, contribute to improving most these limitations. However, this method is hindered relatively signal contrast. Here, gold nanoprisms (AuNPrs) used as amplifiers...
Abstract Active targeting of nanoparticles to tumours can be achieved by conjugation with specific antibodies. Specific active the HER2 receptor is demonstrated in vitro and vivo a subcutaneous MCF-7 breast cancer mouse model trastuzumab-functionalized gold nanoparticles. The number attached antibodies per nanoparticle was precisely controlled way that each conjugated either exactly one or two As expected, we found moderate increase efficiency instead just antibody nanoparticle. However,...
The physicochemical properties of a set 21 different gold nanoparticles (spherical and rod-shaped (NPs) diameters with three surface coatings) were studied. Protein corona formation, in vitro uptake, effect on cell viability proliferation, vivo biodistribution these NPs determined. relation the results was analyzed by hierarchical cluster analysis, which will tell have most similar biological effects, without having to specify individual parameters. show that (Au NPs) are mainly accounted...
Abstract A homologous nanoparticle library was synthesized in which gold nanoparticles were coated with polyethylene glycol, whereby the diameter of cores, as well thickness shell varied. Basic physicochemical parameters this two‐dimensional library, such size, ζ‐potential, hydrophilicity, elasticity, and catalytic activity ,were determined. Cell uptake selected equal size yet varying polymer their effect on basic structural functional cell Data indicates that thinner, more hydrophilic...
Abstract A plasmonic core–shell gold nanostar/zeolitic‐imidazolate‐framework‐8 (ZIF‐8) nanocomposite was developed for the thermoplasmonic‐driven release of encapsulated active molecules inside living cells. The nanocomposites were loaded, as a proof concept, with bisbenzimide functional cargo and wrapped an amphiphilic polymer that prevents ZIF‐8 degradation leaking in aqueous media or demonstrated molecule‐release mechanism relies on use near‐IR light coupled to absorption core nanostars,...
This paper describes a general method to generate noble metal nanoparticles (NPs) with polymer coatings. One of the widely used approaches stabilize NPs in aqueous solution involves wrapping amphiphilic polymers. methodology has been extensively employed for coating small hydrophobic (diameter inorganic core < 20 nm), thereby enabling phase transfer from an organic solvent solution. The approach is herein extended originally synthesized by two-step method. First, are subjected...
In this Perspective, we describe current challenges and recent advances in efficient delivery targeting of nanoparticles vivo. We discuss cancer therapy, nanoparticle-biomolecule interactions, nanoparticle trafficking cells, triggers responses to nanoparticle-cell interactions. No matter which functionalization strategy target is chosen, passive or active targeting, more than 99% the administered vivo end up mononuclear phagocytic system, mainly sequestered by macrophages. Comprehensive...
Exposure of cells to colloidal nanoparticles (NPs) can have concentration-dependent harmful effects. Mostly, such effects are monitored with biochemical assays or probes from molecular biology, i.e., viability assays, gene expression profiles, etc., neglecting that the presence NPs also drastically affect cellular morphology. In case polymer-coated Au NPs, we demonstrate upon NP internalization, undergo lysosomal swelling, alterations in mitochondrial morphology, disturbances actin and...
This study provides a guide to maximizing hysteretic loss by matching the design and synthesis of superparamagnetic nanoparticles desired hyperthermia application. The maximal heat release from magnetic environment depends on intrinsic properties (e.g. size, magnetization, anisotropy), extrinsic applied fields frequency, field strength). Often, biomedical application limits flexibility in setting many parameters nanoparticle size mobility, strength frequency). We show that core-shell...